Antibody response to teichoic acid and peptidoglycan in Staphylococcus aureus osteomyelitis

Jacob, Elliot; Durham, Linda C.; Falk, Michael C.; Williams, Taffy J.; Wheat, L. Joseph

doi:10.1128/jcm.25.1.122-127.1987

Cited by 18 publications

(7 citation statements)

References 34 publications

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“…[16^19]) but the identity of the immunogens, particularly the protein immunogens, of S. aureus still remain to be de¢ned in detail. Most of the published studies have concluded that both uninfected and infected patients have circulating antibodies to S. aureus although there generally are di¡erences in the antibody titres to the bacterium or selected bacterial components [20,21].…”

Section: Discussionmentioning

confidence: 99%

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Royan

2000

FEMS Immunology and Medical Microbiology

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The ability of sera to recognise secreted macromolecules of Staphylococcus aureus was examined by ELISA and Western immunoblotting. Individual secreted proteins were also studied using both human sera and sera from rabbits immunised with secreted macromolecules. Patients sera showed a wide range of IgG antibody titres to secreted macromolecules and whole bacteria. Controls showed a significantly lower IgG response. Western immunoblotting revealed that a significant number of secreted proteins were recognised by circulating IgG antibodies. Surprisingly, both the sera from controls and from patients recognised similar macromolecules including a number of potential virulence factors. The major difference was in the IgG binding to a 16-kDa component, which was recognised by the majority of the sera from infected individuals, but only by a small number of sera from healthy controls. The higher incidence of antibodies recognising the 16 kDa component may be related to our earlier finding that the major bone resorbing component of S. aureus is a heterodimeric protein containing a 16-kDa subunit, the activity of which could be blocked by sera. ß

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Section: Discussionmentioning

confidence: 99%

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Royan

2000

FEMS Immunology and Medical Microbiology

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“…Culture of fluid/pus from sinuses or discharging ulcers is not reliable, thus leading to the dependence of imaging and high suspicion of a surgeon in managing osteomyelitic patients. To determine the causative pathogen implicating in osteomyelitis, a sample of choice is bone fragments because culture of fluid/pus from sinuses or open is always associated with superficial bacteria contaminants [4,5]. This study investigated the pathogens causing osteomyelitis among patients undergoing surgical treatment at Bugando Medical Centre, Mwanza-Tanzania for the purpose of generating data that can be used to devise antibiotic treatment guidelines.…”

Section: Introductionmentioning

confidence: 99%

Methicillin resistant Staphylococcus aureus causing osteomyelitis in a tertiary hospital, Mwanza, Tanzania

Silago¹,

Mushi²,

Remi³

et al. 2020

J Orthop Surg Res

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Background: Culture results of fluid/pus from sinuses or open wound are not reliable in establishing the causative agent of osteomyelitis due to the high chances of contamination of superficial contaminants. Bone fragments obtained during surgery have been recommended as ideal sample to establish pathogens causing osteomyelitis. This study investigated pathogens causing osteomyelitis among patients undergoing orthopedic surgical treatment at Bugando Medical Centre. Methods: A cross-sectional hospital-based study was conducted from December 2017 to July 2018 among 74 patients with osteomyelitis who underwent surgical treatments at Bugando Medical Centre, Mwanza, Tanzania. Bone fragments were collected using sterile 10 ml of in-house prepared brain heart infusion broth (Oxoid, UK) during surgery. Specimens were processed according to standard operating procedures within an hour of collection. Data were analyzed using STATA 13.0. Results: The median age of study participants was 12 with inter quartile range of 8-20 years. The majority 45 (60.8%) of participants were male. All 74 non-repetitive bone fragment specimens had positive culture, of which 17 had dual growth of bacteria resulting to 91 bacterial isolates. Out of 91 isolates, 63 (85.1%) were Staphylococcus aureus (S. aureus) of which 18 (28.6%) were confirmed to be methicillin resistant Staphylococcus aureus strains. Fever was significantly associated with Staphylococcal osteomyelitis (100% vs. 79.6%, p = 0.029). Conclusion: About one third of cases of Staphylococcal osteomyelitis in the current study were caused by methicillin resistant Staphylococcus aureus. There is a need of tailoring antibiotic management of osteomyelitis based on culture and sensitivity results for the better treatment outcome of the patients.

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“…Patients with osteomyelitis do not usually have high levels of antibodies to staphylococcal cell wall antigens. A recent study (14) showed that such patients more frequently had antibodies to P-RTA than to PG. This was also the case in our study, although the fractions of positives for both antigens were low, 38 and 19%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Antibodies to staphylococcal peptidoglycan and its peptide epitopes, teichoic acid, and lipoteichoic acid in sera from blood donors and patients with staphylococcal infections

et al. 1989

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Antibodies to the staphylococcal antigens peptidoglycan, P-ribitol teichoic acid, and lipoteichoic acid, as well as to the peptidoglycan epitopes L-Lys-D-Ala-D-Ala, L-Lys-D-Ala, and pentaglycine, were found over a wide range of concentrations in sera from both blood donors and patients with verified or suspected staphylococcal infections. The patient group was heterogeneous with regard to both age and type of staphylococcal infections, being representative for sera sent to our laboratory. In single-antigen assays antibodies to pentaglycine had the highest predictive positive value (67%), although only 32% of the patients had elevated levels of such antibodies. Combinations of test antigens could yield positive predictive values as high as 100%, but then the fraction of positive sera was low. Indeed, the fraction of patient sera which was positive in multiple-antigen tests never exceeded 61%. The clinical usefulness of these seroassays for identifying Staphylococcus aureus as a causative agent was limited, owing to the considerable overlap in the range of antibody concentrations between patient and blood donor sera.

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Antibody response to teichoic acid and peptidoglycan in Staphylococcus aureus osteomyelitis

Cited by 18 publications

References 34 publications

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Identification of the secreted macromolecular immunogens of Staphylococcus aureus by analysis of serum

Methicillin resistant Staphylococcus aureus causing osteomyelitis in a tertiary hospital, Mwanza, Tanzania

Antibodies to staphylococcal peptidoglycan and its peptide epitopes, teichoic acid, and lipoteichoic acid in sera from blood donors and patients with staphylococcal infections

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