Antibody Responses Against Em18 and Em16 Serodiagnostic Markers in Alveolar and Cystic Echinococcosis Patients From Northwest China

Ito, Akira; Wen, Hao; Craig, Philip S.; Ma, Liang; Nakao, Minoru; Horii, Toshihiro; Pang, Xin-Li; Okamoto, Masanori; Itoh, Makoto; Osawa, Yosuke; Xiao-gen, Wang; Liu, Yuehan

doi:10.7883/yoken1952.50.19

Cited by 11 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results further support our previous observations that Em18 is highly sensitive and species-specific for E. multilocularis. [14][15][16][17][18] The two cases of AE, one from the United States (Alaska) 16 and the other from Poland, that were serologically negative by Em18 and Em2 plus -ELISA, were shown to have calcified inactive lesions only. 26 The nine Chinese CE cases seropositive for anti-Em18 antibody originated from Sichuan, China, where both species of Echinococcus exist 15 and mixed human AE and CE infections have been documented.…”

Section: Resultsmentioning

confidence: 99%

“…A total of 40 and 37 serum samples collected in Switzerland and the United States, respectively, were examined in blind tests for preliminary study. Sixtysix individual serum samples from surgically and/or parasitologically confirmed cases of AE were obtained from China (25), Japan (18), the United States (17), and Poland (6). One hundred seventy-three serum samples from surgically and parasitologically confirmed cases of CE were obtained from China (34), Jordan (36), Australia (39), Turkey (25), the United States (8), Nepal (10), and Poland (21).…”

Section: Methodsmentioning

confidence: 99%

“…Horseradish peroxidase (HRP)-labeled polyclonal antibodies against human IgG and mouse IgG diluted 1:1,000 dilution were used as previously described. [14][15][16][17][18] Preparative isoelectric focusing. The HCF or EmPS crude antigen extracts were fractionated by preparative IEF using the Rotofor cell (Bio-Rad) according to the manufacturer's instructions as described previously.…”

Section: Methodsmentioning

confidence: 99%

“…7,13 Recently, an 18-kD antigen from the E. multilocularis protoscoleces (Em18) was reported by our group as a highly species-specific and sensitive antigen with potential not only for differentiation of AE from either CE or other helminth infections, but also for differentiation of active from inactive AE. [14][15][16][17][18] In general, there was a good correlation between Em18-immunoblots and the Em2 plus -ELI-SA, although the specificity was somewhat higher in the former. 16,18 More recently, we confirmed that both Em18-immunoblots, particularly the IgG subclass Em18-immunoblot and the Em2 plus -ELISA, were reasonably reliable for longterm follow-up study of patients with AE after treatment with albendazole.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).

Itō¹,

Ma²,

Schantz³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

102

View full text Add to dashboard Cite

Abstract. Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.Echinococcosis, one of the most lethal parasitic zoonoses, remains a public health problem of worldwide importance and has recently gained recognition in some regions as an emerging or re-emerging disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).

Itō¹,

Ma²,

Schantz³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

102

View full text Add to dashboard Cite

show abstract

“…The Em2 plus ELISA has been commercialized for clinical diagnosis of AE (103) and for population screening (32). More recently, an 18-kDa antigen (Em18) from PSC of AE was reported as being a highly species-specific (96.8%) and sensitive (97%) antigen with potential not only for differentiation of AE from either CE or other helminth infections but also for differentiation of active from inactive AE (131)(132)(133)(134)(135)(136)143). Both Em2 plus ELISA and Em18 in an IB format have been used for long-term follow-up monitoring of AE patients following pharmacological treatment (173).…”

Section: Brief Comments On the Diagnosis Of Alveolar Echinococcosis Imentioning

confidence: 99%

Concepts in Immunology and Diagnosis of Hydatid Disease

2003

View full text Add to dashboard Cite

Echinococcosis is a cosmopolitan zoonosis caused by adult or larval stages of cestodes belonging to the genus Echinococcus (family Taeniidae). The two major species of medical and public health importance are Echinococcus granulosus and E. multilocularis, which cause cystic echinococcosis (CE) and alveolar echinococcosis (AE), respectively. Both CE and AE are both serious diseases, the latter especially so, with a high fatality rate and poor prognosis if managed inappropriately. This review discusses new concepts and approaches in the immunology and diagnosis of CE, but comparative reference has also been made to AE infection and to earlier pivotal studies of both diseases. The review considers immunity to infection in the intermediate and definitive hosts, innate resistance, evasion of the immune system, and vaccination of intermediate and definitive hosts, and it particularly emphasizes procedures for diagnosis of CE and AE, including the value of immunodiagnostic approaches. There is also discussion of the new advances in recombinant and related DNA technologies, especially application of PCR, that are providing powerful tools in the fields of vaccinology and molecular diagnosis of echinococcosis

show abstract

Performance characteristics and quality control of community based ultrasound surveys for cystic and alveolar echinococcosis

Macpherson

Milner

2003

Acta Tropica

View full text Add to dashboard Cite

Antibody Responses Against Em18 and Em16 Serodiagnostic Markers in Alveolar and Cystic Echinococcosis Patients From Northwest China

Cited by 11 publications

References 10 publications

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).

Concepts in Immunology and Diagnosis of Hydatid Disease

Performance characteristics and quality control of community based ultrasound surveys for cystic and alveolar echinococcosis

Contact Info

Product

Resources

About