Molecules with immunomodulatory properties have been investigated and represent useful tools for understanding the mechanisms involved in the activation and control of immune responses. High molecular weight components (PI) of Ascaris suum extract exert suppressive effect on the immune response to homologous and heterologous antigens, such as ovalbumin (OVA). The PI components exert direct effect on antigen presenting cells (APCs), as dendritic cells (DCS), inhibiting the expression of molecules involved in antigen presentation and thus, decreasing the T lymphocyte proliferation. In addition, this effect is independent of Toll-like receptors 2 and 4 (TLR2 and 4) as well as MyD88 molecule. In this work we studied the glycoconjugates in PI and the participation of C-type lectin receptors (CLRs), mainly the DC-SIGN and MR, in the modulation of the functional activity of DCs. The analysis of anion exchange chromatography (HPAEC-PAD) and mass spectrometry (UV-MALDI-TOF) show that PI components contain high mannose-and complextype N-linked oligosaccharides and phosphorylcholine residues. The binding affinity with distinct lectins showed that part of PI presented affinity to Canavalia ensiformis (ConA), however did not bind to Artocarpus integrifolia (jacalina) suggesting that antigens containing N-linked glycans are abundant in PI. It was observed by flow cytometry that the PI components containing N-linked glycans inhibited the DCs maturation induced by LPS. On the other hand, the previous incubation of DCs with mannan, anti-DC-SIGN and/or anti-MR antibodies abolished the modulatory effect of PI on the DCs maturation. Furthermore, analysys of flow cytometry and confocal microscopy showed that the high binding and internalization of PI-Alexa by DCs was decreased when the cells were previously incubated with mannan, anti-DC-SIGN and/or anti-MR antibodies. It was also observed that the blockage of DC-SIGN and MR in DCs reversed the inhibitory effect of PI in the in vitro T cells proliferative response. Taking together these results show the involvement of DC-SIGN and MR in the recognition of N-glycosylated components of PI by DCs and in its modulatory effect.