Antibody Technics and Blood Coagulation

Hassouna, Houria I.; Penner, John A.

doi:10.1055/s-2007-1005072

Cited by 4 publications

(1 citation statement)

References 212 publications

(341 reference statements)

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“…When tested, the anti-ATIII antibody (anti ATIII ab) had been found to be specific against ATIII and to react with ATIII at a site removed from its thrombin binding site [8]. In this study, the anti ATIII ab/ATIII was used to achieve a better separation of bound ATIII-I'25 thrombin from free thrombin.…”

Section: Determination Of Thrombin Inhibitionmentioning

confidence: 99%

Interaction of protamine sulfate with thrombin

Cobel-Geard

Hassouna

1983

American J Hematol

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Protamine sulfate (salmine), a basic protein with a molecular weight of 4,626 +/- 109, is a known antiheparin agent which in the absence of heparin demonstrates an anticoagulant activity. To date, much work has been done to elucidate the interaction of heparin with thrombin and its physiologic inhibitor, Antithrombin III (ATIII). Little is known, however, about the mechanism of anticoagulant action of protamine sulfate and its mode of thrombin inactivation. We provide information about the interaction of protamine sulfate with purified, labeled thrombin and ATIII through binding experiments in which protamine is shown to inhibit the inactivation of thrombin by ATIII. Furthermore, we show in clotting assays that protamine sulfate has an inhibitory effect on thrombin in the conversion of fibrinogen to fibrin, and that this inhibition is concentration dependent, partial, and reversible.

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Section: Determination Of Thrombin Inhibitionmentioning

confidence: 99%