Antibody Therapy Against Antibiotic-Resistant Diarrheagenic
            <i>Escherichia Coli</i>
            : A Systematic Review

Indhuprakash, Srichandrasekar Thuthikkadu; Karthikeyan, Mukunthan; Gopal, G.; Ambi, Senthil Visaga; Sekaran, Saravanan; Palaniappan, Balamurugan; Diraviyam, Thirumalai

doi:10.2217/imt-2021-0079

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…Among the frequently encountered bacteria, pathogenic Escherichia coli usually causes severe diarrhea worldwide. There are nearly 1.7 billion cases of diarrheal disease reported every year (Yang S. C. et al, 2017 ), and 9.4% of them are caused by diarrheagenic E. coli (Indhuprakash et al, 2021 ), which has resulted in about 760,000 death of children every year from 2011 to 2015 (Chowdhury et al, 2015 ). Methicillin-resistant Staphylococcus aureus (MRSA) is well known as a predominant nosocomial pathogenic bacterium, which represents a crucial crisis in public health systems and poses a serious threat to human life, especially in developed countries (Rebecca, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification, and antibacterial evaluation of endophytic fungi from Gannan navel orange

et al. 2023

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Gannan navel orange is a famous brand in China but the isolation of its endophytic fungi was rarely reported. In this study, a total of 54 strains of endophytic fungi were successfully isolated from the pulp, peel, twig, and leaf of Gannan navel orange; they were successfully identified to belong to 17 species of 12 genera. All these strains were fermented using potato-dextrose agar (PDA) medium, and their secondary metabolites were then extracted with ethyl acetate (EtOAc). The antibacterial assays of Escherichia coli (E. coli), methicillin-resistant Staphylococcus aureus (MRSA), and Xanthomonas citri subsp. citri (Xcc) were also performed for the EtOAc extracts of these strains. As a result, the extracts of both Geotrichum sp. (gc-1-127-30) and Diaporthe biconispora (gc-1-128-79) demonstrated significant antibacterial activities against Xcc, and the MIC value for the extract of Colletotrichum gloeosporioides against MRSA was low to 62.5 μg/mL. Moreover, the chemical components of the extracts of Colletotrichum sp., Diaporthe biconispora, and Annulohypoxylon atroroseum were primarily investigated, and they successfully led to the isolation of 24 compounds involving a new botryane sesquiterpene. Among the isolated products, compound 2 showed significant inhibitory activities toward SA, MRSA, E. coli, and Xcc with MIC values of 12.5, 3.1, 125, and 12.5 μg/mL, respectively. This study revealed that the endophytic fungi of Gannan navel orange showed high potency to produce secondary metabolites with significant antibacterial effects.

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Section: Introductionmentioning

confidence: 99%

Isolation, identification, and antibacterial evaluation of endophytic fungi from Gannan navel orange

et al. 2023

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“…Gram-negative pathogenic Escherichia coli and Salmonella are common pathogens of bacterial disease infections in livestock and poultry farming, which can cause diarrhea diseases and even sepsis in both animals and humans [ 1 , 2 ]. Over four billion episodes of diarrhea occur annually in developing countries with diarrheagenic E. coli outbreaks also being reported [ 3 ]. Salmonella has been reported to cause between 200 million and 1.3 billion cases of intestinal disease worldwide each year [ 4 ], causing hundreds of millions of dollars in losses to the livestock and poultry industry each year [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

C-terminal mini-PEGylation of a marine peptide N6 had potent antibacterial and anti-inflammatory properties against Escherichia coli and Salmonella strains in vitro and in vivo

Yang

Teng

et al. 2022

BMC Microbiol

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Background Enteropathogenic Escherichia coli and Salmonella pullorum are two important groups of zoonotic pathogens. At present, the treatment of intestinal pathogenic bacteria infection mainly relies on antibiotics, which directly inhibit or kill the pathogenic bacteria. However, due to long-term irrational, excessive use or abuse, bacteria have developed different degrees of drug resistance. N6, an arenicin-3 derivative isolated from the lugworm, has potent antibacterial activity and is poorly resistant to enzymatic hydrolysis and distribution in vivo. Polyethylene glycol (PEG) is an extensively studied polymer and commonly used in protein or peptide drugs to improve their therapeutic potential. Here, we modified the N-/C-terminal or Cys residue of N6 with liner PEGn of different lengths (n = 2, 6,12, and 24), and the effects of PEGylation of N6 on the stability, toxicity, bactericidal mechanism, distribution and efficacy were investigated in vitro and in vivo. Results The antimicrobial activity of the peptide showed that PEGylated N6 at the C-terminus (n = 2, N6-COOH-miniPEG) had potent activity against Gram-negative bacteria; PEGylated N6 at the N-terminus and Cys residues showed low or no activity with increasing lengths of PEG. N6-COOH-miniPEG has higher stability in trypsin than the parent peptide-N6. N6-COOH-miniPEG significantly regulated cytokine expression in lipopolysaccharides (LPS)-induced RAW 264.7 cells, and the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β were reduced by 31.21%, 65.62% and 44.12%, respectively, lower than those of N6 (-0.06%, -12.36% and -12.73%); N6-COOH-miniPEG increased the level of IL-10 (37.83%), higher than N6 (-10.21%). The data indicated that N6-COOH-miniPEG has more potent anti-inflammatory and immune-regulatory effect than N6 in LPS-stimulated RAW 264.7 cells. N6-COOH-miniPEG exhibited a much wider biodistribution in mice and prolonged in vivo half-time. FITC-labeled N6-COOH-miniPEG was distributed throughout the body of mice in the range of 0.75 – 2 h after injection, while FITC-labeled N6 only concentrated in the abdominal cavity of mice after injection, and the distribution range was narrow. N6-COOH-miniPEG improved the survival rates of mice challenged with E. coli or S. pullorum, downregulated the levels of TNF-α, IL-6, IL-1β and IL-10 in the serum of LPS-infected mice, and alleviated multiple-organ injuries (the liver, spleen, kidney, and lung), superior to antibiotics, but slightly inferior to N6. Conclusions The antibacterial activity, bactericidal mechanism and cytotoxicity of N6-COOH-miniPEG and N6 were similar. N6-COOH-miniPEG has a higher resistance to trysin than N6. The distribution of N6-COOH-miniPEG in mice was superior to that of N6. In exploring the modulatory effects of antimicrobial peptides on cytokines, N6-COOH-miniPEG had stronger anti-inflammatory and immunomodulatory effects than N6. The results suggested that C-terminal PEGylated N6 may provide an opportunity for the development of effective anti-inflammatory and antibacterial peptides.

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Isolation and identification of endophytic fungi from Alhagi sparsifolia Shap. and their antibacterial activity

Tuerdibieke,

Tian,

et al. 2024

Heliyon

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Antibody Therapy Against Antibiotic-Resistant Diarrheagenic Escherichia Coli : A Systematic Review

Cited by 5 publications

References 76 publications

Isolation, identification, and antibacterial evaluation of endophytic fungi from Gannan navel orange

Isolation, identification, and antibacterial evaluation of endophytic fungi from Gannan navel orange

C-terminal mini-PEGylation of a marine peptide N6 had potent antibacterial and anti-inflammatory properties against Escherichia coli and Salmonella strains in vitro and in vivo

Isolation and identification of endophytic fungi from Alhagi sparsifolia Shap. and their antibacterial activity

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