Antibody titrations are critical for microflow cytometric analysis of extracellular vesicles
Desmond Pink,
Arghya Basu,
Michael Wong
et al.
Abstract:Optimization of flow cytometry assays for extracellular vesicles (EVs) often fail to include appropriate reagent titrations – the most critically antibody titration is either not performed or is incomplete. Using nonoptimal antibody concentration is one of the main sources of error leading to a lack of reproducible data. Antibody titration for the analysis of antigens on the surface of EVs is challenging for a variety of technical reasons. Using platelets as surrogates for cells and platelet‐derived particles … Show more
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