2004
DOI: 10.1073/pnas.0307825100
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Antibody to DNA detects scrapie but not normal prion protein

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Cited by 104 publications
(111 citation statements)
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References 39 publications
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“…supported by experiments showing that FabЈ fragments of some of these antibodies failed to immunoprecipitate PrPsc. This is not surprising taking into account that several proteins and polymers have already been shown to bind SAFs, including serum proteins (12), heparan sulfates (13,14), and nucleic acids (4,15). In addition, we show that a significant PrPsc immunoprecipitation can be obtained even with BSA-coated beads in appropriate conditions.…”
Section: Discussionsupporting
confidence: 70%
“…supported by experiments showing that FabЈ fragments of some of these antibodies failed to immunoprecipitate PrPsc. This is not surprising taking into account that several proteins and polymers have already been shown to bind SAFs, including serum proteins (12), heparan sulfates (13,14), and nucleic acids (4,15). In addition, we show that a significant PrPsc immunoprecipitation can be obtained even with BSA-coated beads in appropriate conditions.…”
Section: Discussionsupporting
confidence: 70%
“…High pressure liquid chromatography-purified synthetic single-stranded RNA fragment 43-59 of SAF93 aptamer (SAF93 [43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59] ) (22) and opRNA, a random 18-mer sequence, derived from a translational operator of MS2 bacteriophage (37) were obtained from Integrated DNA Technologies, Inc. (Coralville, IA). Nucleotide sequences were: SAF93 [43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59] , 5Ј-GGA UGC AAU CUC CAU CCC-3Ј; and opRNA, 5Ј-AAA CAU GGG UUC CCA UGU-3Ј. Synthetic RNA samples were maintained lyophilized at Ϫ20°C and used in RNase-free water.…”
Section: Methodsmentioning
confidence: 99%
“…Notably, Syrian hamster (SHa) Sc237 prions are more readily inactivated by acidic SDS than human sporadic Creutzfeldt-Jakob disease prions by a factor of Ϸ10 5 (23); in contrast, conformationalstability assays were unable to distinguish these two isolates (13,14,24). A limitation of the methodology used in our studies is the need for limited PK digestion, which precludes analysis of proteasesensitive PrP Sc (12,25).…”
Section: Continuum Of Prionsmentioning
confidence: 97%