Antibody to nuclear ribonucleoprotein penetrates live human mononuclear cells through Fc receptors

Alarcón‐Segovia, Donato; Ruı́z-Argüelles, Alejandro; Fishbein, Eugenia

doi:10.1038/271067a0

Cited by 244 publications

(127 citation statements)

References 10 publications

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“…The pathogenicity of lupus antidsDNA Abs is complex and involves several mechanisms such as the binding of extracellular Ags directly or via immune complex formation and/or Ab penetration into living cells (2). Alarcon-Segovia et al (3,4) first suggested that SLE autoantibodies can penetrate the membranes of living cells, react with intracellular targets in the nucleus, and induce functional abnormalities or even cell death.…”

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confidence: 99%

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Calreticulin, a Potential Cell Surface Receptor Involved in Cell Penetration of Anti-DNA Antibodies

Seddiki

Nato

Lafaye

et al. 2001

The Journal of Immunology

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A 50-kDa protein was purified as a potential receptor, using an affinity matrix containing biotinylated F14.6 or H9.3 anti-DNA mAbs derived from autoimmune (New Zealand Black × New Zealand White)F1 mouse and membrane extracts from cells. This protein was identified as calreticulin (CRT) by microsequencing. Confocal microscopy and FACS analysis showed that CRT was present on the surface of various cells. CRT protein was recognized by a panel of anti-DNA mAbs in ELISA. The binding of F14.6 to lymphocytes and Chinese hamster ovary cells was inhibited by soluble CRT or SPA-600. Thus, the anti-DNA mAbs used in this study bound to CRT, suggesting that CRT may mediate their penetration into the cells and play an important role in lupus pathogenesis.

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confidence: 99%

“…S ystemic lupus erythematosus (SLE) 3 is an autoimmune disease typified by the presence of Abs directed against nuclear Ags (1). These lupus antinuclear Abs, include antidsDNA Abs, which are considered to be the hallmark of SLE and are thought to be pathogenic.…”

mentioning

confidence: 99%

Calreticulin, a Potential Cell Surface Receptor Involved in Cell Penetration of Anti-DNA Antibodies

Seddiki

Nato

Lafaye

et al. 2001

The Journal of Immunology

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“…At this point, there was a concern as to whether the antibody could bind to the mitochondria in vital spermatozoa. Since the report by Alarcon-Segovia et al (1978), there has been much 25 research regarding antibody penetration of the cell membrane of living cells and translocation to subcellular compartments (Deng et al, 2000;Yanase et al, 1997). Most of the information is on autoantibodies in autoimmune diseases, but one study reports that antibodies can enter a variety of cell types from different animal species (Deng et al, 2000).…”

Section: Evaluation Of the Sperm Factormentioning

confidence: 99%

The sperm mitochondria-specific translocator has a key role in maternal mitochondrial inheritance

Hayashida

Omagari

Masuda

et al. 2005

Cell Biology International

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AbstrctThe mechanism of maternal mitochondrial inheritance in animals involves the selective elimination of sperm mitochondria by the elimination factor of the egg and the sperm mitochondria-specific factor. In vitro fertilization using sperm from congenic mice incorporating heterogeneous mitochondrial DNA (mtDNA) showed that the number of PCR positives of sperm mtDNA in two-cell embryos was significantly increased following sperm incubation with anti-tpis, anti-Tom22 and anti-Tom40 antibodies. The treatment of fertilized eggs with EGTA and endonuclease inhibitors increased the sperm mtDNA levels. We conclude that the elimination factor, which is probably an endonuclease, is selectively received by the tpis protein of the sperm mitochondrial outer membrane within the egg. It is then transported into the sperm mitochondria by Tom22 and Tom40, where it destroys the sperm mtDNA, establishing the maternal inheritance of mtDNA.3

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“…There is accumulating evidence for the pathogenic role of antidsDNA autoAbs in induction and progress of the diseases (Jang et al 1996;Putterman 2004;Isenberg et al 2007;Im et al 2015). It has been reported by different research groups including ourselves that some autoAbs have cell-penetrating properties, and this subset of Abs could be involved in pathogenesis (Alarcon-Segovia et al 1978;Lee et al 2007;Jang et al 2009;Im et al 2015). However, the mechanism(s) by which cell-penetrating autoAbs contribute to the diseases are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic effect of a cell-penetrating anti-dsDNA autoantibody through p38 signaling pathway and pro-inflammatory cytokine stimulation in mesangial cells

Pravinsagar

Jang

2017

Animal Cells and Systems

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Antibody to nuclear ribonucleoprotein penetrates live human mononuclear cells through Fc receptors

Cited by 244 publications

References 10 publications

Calreticulin, a Potential Cell Surface Receptor Involved in Cell Penetration of Anti-DNA Antibodies

Calreticulin, a Potential Cell Surface Receptor Involved in Cell Penetration of Anti-DNA Antibodies

The sperm mitochondria-specific translocator has a key role in maternal mitochondrial inheritance

Pathogenic effect of a cell-penetrating anti-dsDNA autoantibody through p38 signaling pathway and pro-inflammatory cytokine stimulation in mesangial cells

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