Antibody to Spermine: A Natural Biological Constituent

Bartos, Dagmar; Bartoš, František; Campbell, Richard A.; Grettie, DP; Smejtek, P.

doi:10.1126/science.7375929

Cited by 18 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An antigenicity not associated with the native molecule could also result from structural changes. Were this the case in vivo, it would provide one explanation for the immunogenic stimulus responsible for eliciting natural antibodies to polyamines that have been detected in man (Roch et al, , 1981 and in experimental animals (Bartos et al, 1980;Furuichi et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells

Roch¹,

Quash²,

Ripoll³

et al. 1983

Biochemical Journal

View full text Add to dashboard Cite

1. Fibronectin released from transformed rat kidney cells compared with that released from normal rat kidney cells shows a 50% increase in amino group availability. 2. No such changes were observed in thiol and carboxy group availability or in sialic acid content. 3. The increased amino group availability is not due to a greater polyamine content, which was about 0.04 pmol/mg of protein. 4. Transglutaminase mediated the insertion of spermidine into normal cell fibronectin with linear kinetics. With fibronectin from transformed cells (temperature-sensitive mutant or wild-type), kinetics typical of substrate inhibition were observed. 5. Immunochemical analysis with an anti-polyamine antiserum and an anti-(human fibronectin) antiserum showed that fibronectins from normal and transformed cells react differently. The significance of these results is discussed in the light of changes in the secondary structure between the two fibronectins.

show abstract

Section: Discussionmentioning

confidence: 99%

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells

Roch¹,

Quash²,

Ripoll³

et al. 1983

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The association of exogenous spermine with TYMV is an additional instance of the significant affinity of spermine for all kinds ofanionic surfaces: glass, nucleic acids, cells and organelle membranes, and a number of proteins. Among such spermine-binding proteins, which bind spermine noncovalently much more tightly than spermidine, have recently been described an androgen-sensitive protein in the rat ventral prostate (12) and IgGs of human and rat sera (13)(14)(15). The increased affinity of spermine for certain eukaryotic proteins may relate to the evolutionary origin and survival of spermine biosynthesis in eukaryotic cells and suggests that spermine may regulate the functions of such proteins.…”

Section: Discussionmentioning

confidence: 99%

Spermidine, an intrinsic component of turnip yellow mosaic virus.

Cohen¹,

Greenberg²

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The major polyamine of turnip yellow mosaic virus has been identified as spermidine by gas chromatography and mass spectrometry ofthe trifluoroacetamido derivative. Very small amounts of putrescine and cadaverine, but not norspermidine, have been detected in the virus. The spermidine contents of numerous virus preparations were in the range 200-700 molecules per virion and were considerably in excess of those of spermine. The RNA and spermidine contents of small amounts of the virus were determined after serological precipitation in purified preparations and in the juice of infected plants. Under conditions of the precipitation or purification from juice by differential centrifugation, only small amounts ofexogenous radioactive spermidine and spermine became bound to virus. Although adsorbed radioactive spermidine could be removed almost quantitatively by washing the virus.in dilute buffers, only a small part of adsorbed.spermine was removed by.such treatment. However, >95% of the newly attached spermine was separated from the virus without loss of the original spermidine by sedimentation in buffers containing 0.5 M NaCI or 0.06 M MgCl2. Crystallization of virus in 40% saturated MgS04 or 7.5% heparn or dialysis did not decrease viral spermidine. Although the virus coat may adsorb small amounts ofthe polyamines reversibly, the virus is impermeable to exogenous spermidine and spermine and does not exchange or leak. internal spermidine. The spermidine present in purified virus is. associated. with viral RNA at the time. of packaging and formation of intact virions.Turnip yellow mosaic virus (TYMV), a RNA virus known to contain polyamine (1, 2), is believed to multiply in chloroplast aggregates in Chinese cabbage (3). The virus was reported to contain far more triamine than the tetramine, spermine (1, 2), although the latter is present in the plant and binds to RNA far more tightly than does spermidine (4). The accumulation of putrescine, spermidine, and spermine is exaggerated in the infected plant (5), and we have been investigating the ability of chloroplasts to synthesize the viral triamine. We have reinvestigated the identity of the viral triamine because several recently discovered natural triamines (6), including norspermidine, originally thought to be the viral triamine (1), are not easily separated from spermidine.To determine if the spermidine in the virus was an artifact of preparation, as a result of the sopping up of spermidine into RNA contained within a possibly sponge-like virion, we have studied the adsorption ofpolyamines to virus as well'as the stability ofpolyamine content. The virus does contain spermidine as the sole triamine, which is essentially nonexchangeable, indicating that the triamine is associated with theviral RNA before packaging into virions. MATERIALS AND METHODSGrowth and Purification of TYMV. Chinese cabbage seeds (Brassica pekinensis, var. Pak Choy) were obtained from Nichols Garden Nursery (Albany, OR). Plants were grown in a growth chamber (Scientific Systems, Baton R...

show abstract

“…Natural antibodies to Spm have been shown to be se rum constituents in humans [21] and rabbits [22,23], Such antibodies were detected by applying standard assays to concentrated scrum samples [21][22][23] or by applying spe cially designed assays for measuring the antibody titer of circulating immune complexes after dissociation and reas sociation [11], In the present study, all preimmunc sera were tested for the presence of Spm antibodies without any concentration or dissociation, and no antibody react ing with Spm was detected.…”

Section: Discussionmentioning

confidence: 99%

Immunization of Rabbits with Spermine Induces Antibodies to Self Antigens

Delcros

Muller

et al. 1993

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Rabbits were immunized under different schedules with spermine in the free form or with random noncovalent complexes of spermine or spermidine with ovalbumin. The specificity of the induced antibodies was determined by ELISA and by dot-immunobinding assay. Our results show that in vitro conjugation of spermine and spermidine to a carrier is not an obligatory prerequisite for obtaining corresponding antibodies. Anti-spermine antibodies were found in 9 of 19 animals injected with spermine. Furthermore, all 19 rabbits produced distinct populations of IgM and IgG antibodies which reacted with histones, various synthetic peptides of histones, as well with ubiquitin, a peptide of ubiquitin, dsDNA and two 29-base 5′ synthetic oligodesoxynucleotides. Even in antisera with no detectable reactivity with spermine, antibodies to some of the unrelated antigens were found. The pattern of reactivity of the antisera with the various antigens was different for each immunized animal. These findings lend support to the view that polyamines may play a role in the appearance of autoantibodies.

show abstract

Antibody to Spermine: A Natural Biological Constituent

Cited by 18 publications

References 15 publications

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells

Spermidine, an intrinsic component of turnip yellow mosaic virus.

Immunization of Rabbits with Spermine Induces Antibodies to Self Antigens

Contact Info

Product

Resources

About