Anticancer activity of 23,24-dihydrocucurbitacin B against the HeLa human cervical cell line is due to apoptosis and G2/M cell cycle arrest

Zhang, Jun-Xiao; Hong, Wei-Tan; Hu, Chunyan; Wang, Weiqiang; Chu, Guanghua; Wei, Lihui; Chen, Liu

doi:10.3892/etm.2018.5710

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Ovarian cancer accounts for 0.152 million deaths annually and around 0.3 million new ovarian cases are diagnosed annually worldwide [13][14][15]. The clinical outcome is unsatisfactory due its diagnosis at advanced stages and unavailability of therapeutic targets.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-1179 regulates proliferation and chemosensitivity of human ovarian cancer cells by targeting the PTEN-mediated PI3K/AKT signaling pathway

Chen¹,

Lü²,

Anpeng³

et al. 2020

aoms

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Introduction: Owing to widespread roles of miRs, the dysregulation of their expression in human tissues has been linked with the development of several diseases such as cancer. The study was designed to investigate the role and therapeutic potential of miR-1179 in ovarian cancer. Material and methods: Proliferation rate was monitored by MTT assay. Transfections were performed using Lipofectamine 2000 reagent. Cell cycle apoptosis was detected by AO/EB and annexin V/PI staining. Expressions analysis was carried out by qRT-PCR and western blotting. In vivo evaluation was carried out in xenografted mouse models. Results: The results revealed that miR-1179 is considerably upregulated in ovarian cancer cell lines. Inhibition of miR-1179 triggers decrease in the viability via initiation of apoptotic cell death of ovarian PA-1 cancer cells. Tar-getScan analysis showed PTEN to be the main target of miR-1179 in PA-1 cells. Exploration of PTEN expression in ovarian cancer cell lines revealed up to 9-fold downregulation of PTEN. However, inhibition of miR-1179 in PA-1 cells resulted in upregulation of PTEN expression. In addition, overexpression of PTEN caused a reduction of PA-1 cell viability via induction of apoptotic cell death. However, silencing of miR-1179 could rescue the effects of miR-1179 inhibition on the proliferation of miR-1179. The miR-1179 suppression was accompanied by a significant decline in phosphorylation of PI3K and AKT expression in the PA-1 cells. The in vivo study showed that miR-1179 suppression inhibits the xenografted tumor growth. Conclusions: The results of this study indicate that miR-1179 may prove to be an important therapeutic target for ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-1179 regulates proliferation and chemosensitivity of human ovarian cancer cells by targeting the PTEN-mediated PI3K/AKT signaling pathway

Chen¹,

Lü²,

Anpeng³

et al. 2020

aoms

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“…The phosphoinositide 3 kinase/protein kinase B/mechanistic target of rapamycin (PI3K/AKT/mTOR) cascade may play an important role in cancer tumorigenesis and progression and resistance to chemotherapy. The results indicated that DHCB decreased the expression of important proteins in the PI3K/Akt/mTOR cascade [16].…”

Section: Cucurbitacin B (1′)mentioning

confidence: 94%

Cytotoxic and Antitumoral Activities of Compounds Isolated from Cucurbitaceae Plants

Méndez-Cuesta¹,

Campos²,

Sánchez³

et al. 2019

Pharmacognosy - Medicinal Plants

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The WHO says that annual cases of cancer will increase from 14 million in 2012 to 22 million in the next two decades. Cancer is the second cause of death in the world; in 2015, it caused 8.8 million deaths. On the other hand, it is necessary to consider that 70% of the total deaths due to this disease occur in developing countries, who have the least resources to acquire the drugs of choice for the treatment of this disease. Although there are treatments and these are effective, there are currently cases of resistance to drugs used to treat this disease, which has led to the search for new sources of drugs or compounds effective against the cancer being active; plants are the possible sources to achieve this. Cucurbitaceae is a family of plants widely distributed on the planet which has been used traditionally for the treatment of this disease and from they have been isolated different cucurbitanes. These compounds possess a wide biological activity, antidiabetic, anti-inflammatory, hepatoprotective, or cytotoxic and antitumoral effects. The aim of this review is to present 51 cucurbitacin compounds and 2 with different structures isolated from Cucurbitaceae plants with cytotoxic or antitumoral activity.

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“…HeLa is named so after Henrietta Lacks, a lady who died of cervical cancer in 1951 (13). Ever since the distribution of her cells, this cell line has been commonly used to evaluate the effects of various anticancer drugs (14). HeLa cells have been used to investigate the phytocomponents (plant-based components) and the apoptotic mechanism of the anti-cancer activity of the ethanolic extract of mango peel (15).…”

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confidence: 99%

A study to determine the anti-cancer and pro-apoptotic properties of Amaranthus spinosus Linn. Extract, AS20

Sharma,

Kasture,

Umrao

et al. 2020

J Emerg Invest

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Complementary approaches to cancer treatment seek methods that integrate into existing therapies such as chemotherapy and radiotherapy. The most common form of complementary medicine is the usage of external antioxidants, including Ayurvedic herbal extracts, which assist in the mitigation of the side effects of cancer treatment on healthy cells. Herbal extracts are usually evaluated only for their antioxidant properties for complementary use in cancer treatment. Ayurvedic products are rarely evaluated for their anti-cancer properties. In this paper, we hypothesized that AS20, a crude extract of the whole Amaranthus spinosus (AS) plant, would induce higher cell death to the HeLa cancer cell line compared to other extracts of individual parts of the plant. Significantly lower IC50 values were found for both 24 hours (11.56 µg/ml) and 48 hours (1.77 µg/ml) exposure compared to any of the other individual extracts of the plant part for the given exposure duration. Also, apoptosis was observed in HeLa cells treated by the IC50 concentration of AS20 using fluorescent stains such as Hoechst dye, DAPI and dual staining (Acridine Orange and Propidium Iodide). It is thus concluded that AS20 formulation exhibited cytotoxic activity on HeLa cancer cell lines and their action mechanism is via apoptosis.

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Anticancer activity of 23,24-dihydrocucurbitacin B against the HeLa human cervical cell line is due to apoptosis and G2/M cell cycle arrest

Cited by 5 publications

References 27 publications

MicroRNA-1179 regulates proliferation and chemosensitivity of human ovarian cancer cells by targeting the PTEN-mediated PI3K/AKT signaling pathway

MicroRNA-1179 regulates proliferation and chemosensitivity of human ovarian cancer cells by targeting the PTEN-mediated PI3K/AKT signaling pathway

Cytotoxic and Antitumoral Activities of Compounds Isolated from Cucurbitaceae Plants

A study to determine the anti-cancer and pro-apoptotic properties of Amaranthus spinosus Linn. Extract, AS20

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