Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells

Krishnankutty, Roopesh; Iskandarani, Ahmad; Therachiyil, Lubna; Uddin, Shahab; Azizi, Fouad; Kulinski, Michael; Bhat, Ajaz A.; Mohammad, Ramzi M.

doi:10.31557/apjcp.2018.19.12.3501

Cited by 48 publications

(43 citation statements)

References 33 publications

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“…Anti-proliferative effects of milk proteins have been studied earlier and are emerging as highly promising agents to overcome the relapse and side effects of chemotherapy 21 . Camel milk is known to induce growth arrest in breast cancer cells 22 and trigger significant apoptosis effect in HepG2 cells 23 . 18 CWPHs were screened for anti-cancer activity on HepG2 cells (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

Murali

Mudgil

Gan

et al. 2021

Sci Rep

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Camel milk has been gaining immmense importance due to high nutritious value and medicinal properties. Peptides from milk proteins is gaining popularity in various therapeutics including human cancer. The study was aimed to investigate the anti-cancerous and anti-inflammatory properties of camel whey protein hydrolysates (CWPHs). CWPHs were generated at three temperatures (30 ℃, 37 ℃, and 45 ℃), two hydrolysis timepoints (120 and 360 min) and with three different enzyme concentrations (0.5, 1 and 2 %). CWPHs demonstrated an increase in anti-inflammatory effect between 732.50 (P-6.1) and 3779.16 (P-2.1) µg Dicolfenac Sodium Equivalent (DSE)/mg protein. CWPHs (P-4.3 & 5.2) inhibited growth of human colon carcinoma cells (HCT116) with an IC50 value of 231 and 221 μg/ml, respectively. P-4.3 induced G2/M cell cycle arrest and modulated the expression of Cdk1, p-Cdk1, Cyclin B1, p-histone H3, p21 and p53. Docking of two peptides (AHLEQVLLR and ALPNIDPPTVER) from CWPHs (P-4.3) identified Polo like kinase 1 as a potential target, which strongly supports our in vitro data and provides an encouraging insight into developing a novel peptide-based anticancer formulation. These results suggest that the active component, CWPHs (P-4.3), can be further studied and modeled to form a small molecule anti-cancerous therapy.

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Section: Resultsmentioning

confidence: 99%

Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

Murali

Mudgil

Gan

et al. 2021

Sci Rep

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“…Thus, in the condition where ROS overwhelms antioxidant systems, the protection against oxidative damage is achieved by exogenous supplementation of antioxidants [ 69 ]. Camel milk is an excellent exogenous antioxidant supplement that can be used to attenuate the oxidative stress associated with many diseases, such as hepatitis and cancers [ 70 , 71 , 72 ]. The caseins, LAB, bioactive peptides and whey proteins, especially lactoferrin, are the major constituents of camel milk having the antioxidant properties…”

Section: The Anti-microbial and Antioxidant Properties Of Camel Milkmentioning

confidence: 99%

Research Development on Anti-Microbial and Antioxidant Properties of Camel Milk and Its Role as an Anti-Cancer and Anti-Hepatitis Agent

Khan

Xiao

et al. 2021

Antioxidants

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Camel milk is a rich source of vitamin C, lactic acid bacteria (LAB), beta-caseins and milk whey proteins, including lactoferrin, lysozyme, lactoperoxidase, alpha-lactalbumin and immunoglobulin. The lactoferrin plays a key role in several physiological functions, such as conferring antioxidant, anti-microbial and anti-inflammatory functions in cells. Similarly, the camel milk alpha-lactalbumin has shown greater antioxidative activity because of its higher antioxidant amino acid residues. The antioxidant properties of camel milk have also been ascribed to the structural conformation of its beta-caseins. Upon hydrolysis, the beta-caseins lead to some bioactive peptides having antioxidant activities. Consequently, the vitamin C in camel milk has a significant antioxidant effect and can be used as a source of vitamin C when the climate is harsh. Furthermore, the lysozyme and immunoglobulins in camel milk have anti-microbial and immune regulatory properties. The LAB isolated from camel milk have a protective role against both Gram-positive and -negative bacteria. Moreover, the LAB can be used as a probiotic and may restore the oxidative status caused by various pathogenic bacterial infections. Various diseases such as cancer and hepatitis have been associated with oxidative stress. Camel milk could increase antiproliferative effects and regulate antioxidant genes during cancer and hepatitis, hence ameliorating oxidative stress. In the current review, we have illustrated the anti-microbial and antioxidant properties of camel milk in detail. In addition, the anti-cancer and anti-hepatitis properties of camel milk have also been discussed.

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“…Indeed, different oncogenic alterations can activate common metabolic pathways. 3 We therefore began investigating how metabolism mediates RT-resistance in GBM.…”

Section: Textmentioning

confidence: 99%

Purine metabolism promotes radioresistance and is a therapeutic target in glioblastoma

Zhou

Wahl

2020

Molecular & Cellular Oncology

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Profound intratumoral genomic heterogeneity has limited the ability of targeted therapies to overcome therapy resistance in glioblastoma. We have defined purine metabolism as a key mediator of DNA repair and radiation resistance in glioblastoma. Because many glioblastoma oncogenic drivers activate purine metabolism, its inhibition may overcome therapy resistance despite intratumoral genomic heterogeneity.

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Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells

Cited by 48 publications

References 33 publications

Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

Research Development on Anti-Microbial and Antioxidant Properties of Camel Milk and Its Role as an Anti-Cancer and Anti-Hepatitis Agent

Purine metabolism promotes radioresistance and is a therapeutic target in glioblastoma

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