Anticancer activity of fish oils against human lung cancer is associated with changes in formation of PGE<sub>2</sub> and PGE<sub>3</sub> and alteration of Akt phosphorylation

Yang, Peiying; Cartwright, Carrie; Chan, Diana; Ding, Jibin; Felix, Edward; Pan, Yong; Pang, Jihai; Rhea, Patrea R.; Block, Keith I.; Fischer, Susan M.; Newman, Robert A.

doi:10.1002/mc.22008

Cited by 56 publications

(46 citation statements)

References 47 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, when COX-2 expression was reduced by siRNA or shRNA in A549 cells, the antiproliferative activity of EPA was also reduced. These results suggest that the ability of EPA to generate PGE3 through the COX-2 enzyme might be critical for EPA-mediated of lung cancer cells via down-regulation of Akt phosphorylation by PGE3 [170].…”

Section: Lung Cancermentioning

confidence: 80%

“…A recent work suggested that this antiproliferative effect can be due in part to selective alteration of arachidonate metabolism that involves COX enzymes. EPA inhibited 50 % of proliferation of A549 cells (COX-2 over-expressing) at 6.05 μM, while almost 80 μM was needed to reach similar levels of inhibition of H1299 cells (COX-2 null) [170]. Accordingly, the formation of prostaglandin (PG)E3 in A549 cells was almost 3-fold higher than that of H1299 cells when they were treated with EPA (25 μM).…”

Section: Lung Cancermentioning

confidence: 97%

See 1 more Smart Citation

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Nabavi

Bilotto

Russo

et al. 2015

Cancer Metastasis Rev

140

View full text Add to dashboard Cite

Over the past decades, extensive studies have addressed the therapeutic effects of omega-3 polyunsaturated fatty acids (omega-3 FAs) against different human diseases such as cardiovascular and neurodegenerative diseases, cancer, etc. A growing body of scientific research shows the pharmacokinetic information and safety of these natural occurring substances. Moreover, during recent years, a plethora of studies has demonstrated that omega-3 FAs possess therapeutic role against certain types of cancer. It is also known that omega-3 FAs can improve efficacy and tolerability of chemotherapy. Previous reports showed that suppression of nuclear factor-κB, activation of AMPK/SIRT1, modulation of cyclooxygenase (COX) activity, and up-regulation of novel anti-inflammatory lipid mediators such as protectins, maresins, and resolvins, are the main mechanisms of antineoplastic effect of omega-3 FAs. In this review, we have collected the available clinical data on the therapeutic role of omega-3 FAs against breast cancer, colorectal cancer, leukemia, gastric cancer, pancreatic cancer, esophageal cancer, prostate cancer, lung cancer, head and neck cancer, as well as cancer cachexia. We also discussed the chemistry, dietary source, and bioavailability of omega-3 FAs, and the potential molecular mechanisms of anticancer and adverse effects.

show abstract

Section: Lung Cancermentioning

confidence: 80%

Section: Lung Cancermentioning

confidence: 97%

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Nabavi

Bilotto

Russo

et al. 2015

Cancer Metastasis Rev

140

View full text Add to dashboard Cite

show abstract

“…For some other types of cancer cells, Kim et al 42 studied the effect of DHA standard (10-60 µM) on the growth of lung cancer cells H1299 and A549; a dose-dependent decrease in cell viability was observed with the IC 50 being 46 and 40 µM, respectively. Similarly, the IC 50 was 50 and 9.7 µM, respectively, after the treatment of lung cancer cells H1299 and A549 with DHA standard (1-100 µM) 43 and was 162.5 µM for liver cancer cells MHCC97L when treated with DHA standard (0-200 µM). 44 For breast cancer cells MCF-7, MDA-MB-231, and MDA-MB-435, the IC 50 were 20.2, 57.4, and 70 µM, respectively, when treated with DHA.…”

Section: Cell Viability Of B16-f10 and Ccd986sk Cellsmentioning

confidence: 92%

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Yang¹,

Hung²,

Chen³

2017

IJN

View full text Add to dashboard Cite

Coffee grounds, a waste by-product generated after making coffee, contains approximately 15% coffee oil which can be used as a raw material in cosmetics. Algae oil rich in docosahexaenoic acid (DHA) has been demonstrated to possess anticancer and anti-inflammation functions. The objectives of this study were to develop a gas chromatography-mass spectrometry (GC-MS) method for the determination of fatty acids in coffee oil and algae oil and prepare a nanoemulsion for studying its inhibition effect on ultraviolet A-induced skin damage in mice and growth of melanoma cells B16-F10. A total of 8 and 5 fatty acids were separated and quantified in coffee oil and algae oil by GC-MS, respectively, with linoleic acid (39.8%) dominating in the former and DHA (33.9%) in the latter. A nanoemulsion with a particle size of 30 nm, zeta potential −72.72 mV, and DHA encapsulation efficiency 100% was prepared by using coffee oil, algae oil, surfactant (20% Span 80 and 80% Tween 80), and deionized water. Differential scanning calorimetry (DSC) analysis revealed a high stability of nanoemulsion when heated up to 110°C at a pH 6, whereas no significant changes in particle size distribution and pH occurred over a 90-day storage period at 4°C. Animal experiments showed that a dose of 0.1% coffee oil-algae oil nanoemulsion was effective in mitigating trans-epidermal water loss, skin erythema, melanin formation, and subcutaneous blood flow. Cytotoxicity test implied effective inhibition of melanoma cell growth by nanoemulsion with an IC 50 value of 26.5 µg/mL and the cell cycle arrested at G2/M phase. A dose-dependent upregulation of p53, p21, cyclin B, and cyclin A expressions and downregulation of CDK1 and CDK2 occurred. Also, both Bax and cytochrome c expressions were upregulated and bcl-2 expression downregulated, accompanied by a rise in caspase-3, caspase-8, and caspase-9 activities for apoptosis execution. Collectively, the apoptosis pathway of melanoma cells B16-F10 may involve both mitochondria and death receptor.

show abstract

“…Similarly, Xia et al and Hawcroft et al reported that the formation of PGE 3 in EPA treated mouse melanoma and human HCA-7 CRC cells was blocked by indomethacin [79] or a selective COX-2 inhibitor, SC-236, respectively [24]. The important role of COX-2 in regulating PGE 3 metabolism was further supported by the finding that a substantially lower amount of PGE 3 was detected in the H1299 cells (COX-2 null NSCLC cells) and its xenograft tumor tissues compared to that in A549 cells (COX-2 constitutively expressed) and its xenograft [80]. The formation of PGE 3 was partially blocked in the COX-2 knockdown A549 cells compared with the control siRNA administrated A549 cells.…”

Section: Cpla2 Cox-2 and Pge3 Metabolism In Cancermentioning

confidence: 99%

Prostaglandin E3 metabolism and cancer

2014

Self Cite

View full text Add to dashboard Cite

The anticancer activity of n-3 fatty acids, especially those derived from fish, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid) (DHA), has been studied for centuries. While there is a growing body of evidence that EPA and DHA may influence cancer initiation and development through targeting multiple events of tumor development, the underlying mechanisms responsible for these activities are still not fully understood. A number of studies have suggested that the anticancer activities of EPA and DHA are associated with their effects on eicosanoid metabolism by which they inhibit prostaglandin E2 (PGE2) production. In contrast to DHA, EPA can function as a substrate for cyclooxygenases (COXs) to synthesize unique 3-series prostaglandin compounds, especially PGE3. With advance technology in mass spectrometry, there is renewed interest in studying the role of PGE3 in EPA elicited anti-proliferative activity in various cancers, with some promising results. Here, we summarize the regulation of PGE3 synthesis in cancer cells and its role in EPA elicited anticancer activity. The development of PGE3 and its metabolites as potential biomarkers for future clinical evaluation of EPA and fish oil in cancer care is discussed.

show abstract

Anticancer activity of fish oils against human lung cancer is associated with changes in formation of PGE₂ and PGE₃ and alteration of Akt phosphorylation

Cited by 56 publications

References 47 publications

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Prostaglandin E3 metabolism and cancer

Contact Info

Product

Resources

About

Anticancer activity of fish oils against human lung cancer is associated with changes in formation of PGE2 and PGE3 and alteration of Akt phosphorylation

Cited by 56 publications

References 47 publications

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Prostaglandin E3 metabolism and cancer

Contact Info

Product

Resources

About

Anticancer activity of fish oils against human lung cancer is associated with changes in formation of PGE₂ and PGE₃ and alteration of Akt phosphorylation