Anticancer Activity of Flavone of Excoecaria agallocha in Cytotoxicity Studies Using Hela Cells

Rajeswari, K.; Rao, T. Bhaskara; Sharma, Gunjan; Krishna, R. Murali

doi:10.14233/ajchem.2017.20724

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…Cytotoxicity assays showed that sorafenib is a potent toxicant to cells in both lines, but stigmasterol cytotoxicity was weak; an IC 50 was not estimated since even high concentrations did not cause 50% lethality. Previous studies reported similar results for sorafenib [19] and stigmasterol [20] cytotoxicity in breast cancer.…”

Section: Discussionsupporting

confidence: 74%

The anti-tumor effect of stigmasterol on sorafenib treated human breast cancer cell lines

elshamy

Omran

Abd‐Αlhaseeb

et al. 2021

Preprint

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Background: Breast cancer is the most common invasive malignancy and the leading cause of tumor-related death among women globally. Excessive angiogenesis, sustained proliferation, and evasion of apoptosis are crucial for breast cancer progression. Sorafenib is a multi-kinase receptor inhibitor with anti-angiogenic activity. Stigmasterol is a phytosterol with anticancer activity. The aim of this study was to investigate molecular mechanisms of action of sorafenib and stigmasterol in two different human breast cancer cell lines and assess their combined impact on modulation of signaling pathways that control breast cancer pathogenesis. Methods and results: MCF-7 and MDA-MB 231 cells were used as models of human breast cancer. MTT assay was used to assess cytotoxicity. Angiogenic VEGF/ VEGFR-2/ ERK/ NF-kB signaling pathway was investigated using ELISA and RT-PCR techniques. Apoptotic markers (caspase-3, Bcl2) and a proliferation marker (Ki-67) were assessed using colorimetric and ELISA techniques. Sorafenib combined with stigmasterol increased caspase-3 activity and decreased Bcl2, VEGF-A, VEGFR-2, NF-kB and Ki-67 levels. Conclusion: The combination of Sorafenib and stigmasterol may be a useful therapeutic regimen for breast cancer treatment. This combination may inhibit angiogenesis and promote apoptosis signaling.

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Section: Discussionsupporting

confidence: 74%

The anti-tumor effect of stigmasterol on sorafenib treated human breast cancer cell lines

elshamy

Omran

Abd‐Αlhaseeb

et al. 2021

Preprint

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“…Rajeswari et al . ( 27 ) also reported that the flavone molecule from Excoecaria agallocha has the cytotoxic activity against HeLa cells.…”

Section: Resultsmentioning

confidence: 94%

Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells

Venkateswarulu

Eswaraiah

Krupanidhi

et al. 2020

Food technol. biotechnol. (Online)

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Mangroves contain a wide range of bioactive compounds with pharmacological activities. In the present study, we analysed the separation and detection of phytoconstituents with the methanol extract of Ipomoea tuba leaf using gas chromatography-mass spectrometry (GC-MS) and tested its in vitro cytotoxicity effect against MCF-7 and HeLa cells. Phytochemical compounds such as docosanoic, octadecatrienoic and cis-9-octadecanoic acids, triterpenoid γ-sitosterol, and terpene alcohol in methanol extract of I. tuba leaf were identified. Furthermore, in vitro antiproliferative activity of the extract of I. tuba leaf was evaluated using MCF-7 and HeLa cells. The results indicated a reduction of cell viability of 37.43 and 41.89 % of MCF-7 and HeLa cells respectively. The methanol extract of I. tuba leaf proved to be effective in protecting the cells against oxidative stress. This is the first report on the in vitro cytotoxicity effect of I. tuba leaf extract on MCF-7 and HeLa cells.

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“…To this 20 µL of MTT (2 mg/mL MTT in PBS) was added and further incubated for 4 hours at room temperature in a 5% CO 2 environment. About 100 µL DMSO was gently mixed into the plate to dissolve the formazan and absorbance read at 570 nm (15).…”

Section: In-vitro Cytotoxic Activity By Mtt Assaymentioning

confidence: 99%

In-vitro Antioxidant and Cytotoxic Effects of Physalis minima Linn. in HeLa Cell Lines Against Cervical Cancer

Velemurugan,

Sethuraman,

Kamaraj

2024

Curr. Trends Biotechnol. Pharm.

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In the present study, the shade-dried and powdered leaves of Physalis minima Linn. were subjected to Soxhlet extraction with methanol, concentrated, and further partitioned with n-hexane, chloroform, and ethanol. The in-vitro anti-oxidant activity was evaluated by DPPH radical scavenging assay and the cytotoxic potential by MTT assay in HeLa cell lines. The results of the study revealed that among the three fractions, the chloroform fraction of the methanolic extract showed better antioxidant activity (IC 50 = 38.6 µg/mL) compared to standard Ascorbic acid (IC 50 = 42.6 µg/mL). Moreover, the results of the MTT assay carried out in HeLa cell lines showed that the chloroform fraction exhibited a promising cytotoxic activity (IC 50 = 59.66 µg/mL). Further, the chloroform fraction when subjected to apoptosis assay using acridine orange -ethidium bromide dual staining displayed the morphological characteristics of apoptotic event including nuclear fragmentation and chromatin condensation with orange red and reddish nuclei. The results of our study support the protective effect of chloroform fraction of methanolic extract of leaves of Physalis minima Linn., in the treatment of cervical cancer.

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Anticancer Activity of Flavone of Excoecaria agallocha in Cytotoxicity Studies Using Hela Cells

Cited by 6 publications

References 14 publications

The anti-tumor effect of stigmasterol on sorafenib treated human breast cancer cell lines

The anti-tumor effect of stigmasterol on sorafenib treated human breast cancer cell lines

Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells

In-vitro Antioxidant and Cytotoxic Effects of Physalis minima Linn. in HeLa Cell Lines Against Cervical Cancer

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