2008

DOI: 10.3748/wjg.14.627

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Anticancer activity of genistein on implanted tumor of human SG7901 cells in nude mice

Jin‐Ming Chen²,

et al.

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Cited by 35 publications

(23 citation statements)

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“…The ability of cancer cells to form metastases depends on a set of unique biological properties that enable the malignant cells to complete all these steps of the metastatic process. Currently, only a few chemotherapeutic drugs are effective for the treatment of patients with malignant tumor metastasis, and there is a clear need to identify new antimetastatic drugs [9] . Genistein, an isoflavonoid abundant in soy beans, is a planar molecule with an aromatic A ring, has a second oxygen atom from that in the A ring, and has a molecular mass similar to those of the steroidal estrogens [9][10][11] .…”

Section: Discussionmentioning

confidence: 99%

“…Currently, only a few chemotherapeutic drugs are effective for the treatment of patients with malignant tumor metastasis, and there is a clear need to identify new antimetastatic drugs [9] . Genistein, an isoflavonoid abundant in soy beans, is a planar molecule with an aromatic A ring, has a second oxygen atom from that in the A ring, and has a molecular mass similar to those of the steroidal estrogens [9][10][11] . Reports from epidemiological and experimental studies show that it plays an important role in the inhibition of tumors including breast cancer, prostate cancer, colon cancer, leukemia and melanoma [12,13] .…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

Gu¹,

Zhu²,

Dai³

et al. 2009

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AIM:To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS:MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed. RESULTS:Genistein significantly inhibited the growth of MHCC97-H cells in vitro . Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentrationdependent fashion, and the inhibitory effect of genistein was more potent in the 10 μg/mL and 20 μg/ mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic foci in the genistein group was significantly lower compared with the control group (12.3 ± 1.8 vs 16.6 ± 2.6, P < 0.05).CONCLUSION: Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.

“…The ability of cancer cells to form metastases depends on a set of unique biological properties that enable the malignant cells to complete all these steps of the metastatic process. Currently, only a few chemotherapeutic drugs are effective for the treatment of patients with malignant tumor metastasis, and there is a clear need to identify new antimetastatic drugs [9] . Genistein, an isoflavonoid abundant in soy beans, is a planar molecule with an aromatic A ring, has a second oxygen atom from that in the A ring, and has a molecular mass similar to those of the steroidal estrogens [9][10][11] .…”

Section: Discussionmentioning

confidence: 99%

“…Currently, only a few chemotherapeutic drugs are effective for the treatment of patients with malignant tumor metastasis, and there is a clear need to identify new antimetastatic drugs [9] . Genistein, an isoflavonoid abundant in soy beans, is a planar molecule with an aromatic A ring, has a second oxygen atom from that in the A ring, and has a molecular mass similar to those of the steroidal estrogens [9][10][11] . Reports from epidemiological and experimental studies show that it plays an important role in the inhibition of tumors including breast cancer, prostate cancer, colon cancer, leukemia and melanoma [12,13] .…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

Gu¹,

Zhu²,

Dai³

et al. 2009

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AIM:To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS:MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed. RESULTS:Genistein significantly inhibited the growth of MHCC97-H cells in vitro . Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentrationdependent fashion, and the inhibitory effect of genistein was more potent in the 10 μg/mL and 20 μg/ mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic foci in the genistein group was significantly lower compared with the control group (12.3 ± 1.8 vs 16.6 ± 2.6, P < 0.05).CONCLUSION: Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.

“…In the USA and European studies, viral hepatitis C was shown to be a risk factor for CCA with the strongest association observed for intrahepatic CCA [60], and a Japanese study subsequently conirmed these indings [61]. However, studies from South Korea and China have shown that hepatitis B is a more consistent risk factor for intrahepatic CCA [62][63][64]. A meta-analysis of several case-control studies on risk factors for intrahepatic CCA showed that the combined odds ratios (ORs) [65].…”

Section: Updates In Liver Cancermentioning

confidence: 90%

Cancer Stem Cells and Aldehyde Dehydrogenase 1 in Liver Cancers

et al. 2017

Updates in Liver Cancer

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The cancer stem cell (CSC) theory posits that a small population of cells with stem celllike features is responsible for tumor growth, resistance, and recurrence in many malignancies. This theory could be a useful paradigm for designing innovative targeted drug therapies. Liver cancer is the ifth most common cancer worldwide, with hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) as the predominant forms. Hepatic stem/progenitor cells are believed to be the origin of HCCs and CCAs; however, this remains a controversial topic. Aldehyde dehydrogenase (ALDH) is the main enzymatic system responsible for the clearance of acetaldehyde from the hepatocytes in the liver tissue. Therefore, ALDH1 has been suggested to be a potential, biological and CSC marker in liver cancers. We here provide an overview of the current state of knowledge of CSCs in liver and the role of ALDH1 in the development and progression of liver cancers and discuss its potential value as a prognostic and diagnostic biomarker.

“…Currently only a few chemotherapeutic drugs are effective for the treatment of human gastric carcinoma [13] and there is an increasing interest in the use of drugs to prevent its occurrence or invasiveness. The lipid kinase PI3K is a proto-oncogene that generates 3'-phosphoinositides at the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Effects of LY294002 on the invasiveness of human gastric cancer in vivo in nude mice

Chen¹,

Zhu²,

Fan³

et al. 2009

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AIM:To investigate the effects of class Ⅰ phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 on the invasiveness and related mechanisms of implanted tumors of SGC7901 human gastric carcinoma cells in nude mice. METHODS:Nude mice were randomly divided into model control groups and LY294002 treatment groups. On days 5, 10 and 15 after treatment, the inhibitory rate of tumor growth, pathological changes in tumor specimens, expression levels of matrix metalloproteinase (MMP)-2, MMP-9, CD34 [representing microvessel density (MVD)] and vascular endothelial growth factor (VEGF), as well as apoptosis indexes in tumor samples were observed. RESULTS:In this study, we showed that treating the tumors with LY294002 could significantly inhibit carcinoma growth by 11.3%, 29.4% and 36.7%, after 5, 10 and 15 d, respectively, compared to the control group. Hematoxylin & eosin staining indicated that the rate of inhibition increased progressively (23.51% ± 3.11%, 43.20% ± 3.27% and 63.28% ± 2.10% at 5, 10 and 15 d, respectively) along with apoptosis. The expression of MMP-2 was also downregulated (from 71.4% ± 1.6% to 47.9% ± 0.7%, 31.9% ± 0.9% and 7.9% ± 0.7%). The same effects were observed in MMP-9 protein expression (from 49.4% ± 1.5% to 36.9% ± 0.4%, 23.5% ± 0.9% and 7.7% ± 0.6%), the mean MVD (from 51.2% ± 3.1% to 41.9% ± 1.5%, 30.9% ± 1.7% and 14.9% ± 0.8%), and the expression of VEGF (from 47.2% ± 3.1% to 25.9% ± 0.5%, 18.6% ± 1.2% and 5.1% ± 0.9%) by immunohistochemical staining. CONCLUSION:The class Ⅰ PI3K inhibitor LY294002 could inhibit the invasiveness of gastric cancer cells by downregulating the expression of MMP-2, MMP-9, and VEGF, and reducing MVD.

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