Anticancer Activity of Hispidulin from Saussurea simpsoniana and Lupeol from Vincetoxicum arnottianum

Hassan, Ghulam Mubashar; Zahra, Qandeel; Engel, Nadja; Ali, Kiran Amir; Bahreen, Gul; Ahmad, V. U.; Aziz, Shahid R.

doi:10.26538/tjnpr/v4i2.2

Cited by 3 publications

(5 citation statements)

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“…It is known as a multi-target agent, similar to Plumbagin, and has biological activities as anticancer and anti-inflammatory. 18,19 Furthermore, a small amount of Plumbagin was found in the chloroform extract of PZCJ (Table 2). Previous studies reported that the leaf part of P. zeylanica L. from Tamil Nadu, India contained Plumbagin up to 0.00007%.…”

Section: Resultsmentioning

confidence: 99%

Identification of Chemical Compounds in Plumbago zeylanica Linn Leaves from Indonesia

2022

TJNPR

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Plumbago zeylanica L., commonly known as leadwort or Ki Encok plant in Indonesia, has some beneficial effects on human health, such as anticancer, anti-microbial, anti-inflammatory, wound healing, and anti-diabetic. Therefore, this experimental study aims to evaluate the chloroform extracts of P. zeylanica L. leaves for the isolation of the phytoconstituents. The crushed form of the leaves from South Sumatera (6.5 g) was macerated in 65 ml of chloroform for 5 days, while a total of 80 g leaves powder from Central Java was dissolved in 800 ml of chloroform. The extracts were filtered and evaporated to dryness using a rotary evaporator. Subsequently, the identification of secondary metabolites was carried out using Gas Chromatography-Mass Spectrometry (GC-MS). In conclusion, the leaves of P. zeylanica L. contain -sitosterol, lupeol, plumbagin, phytol, and lauric acid.

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Section: Resultsmentioning

confidence: 99%

Identification of Chemical Compounds in Plumbago zeylanica Linn Leaves from Indonesia

2022

TJNPR

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“…[ 21 , 39 ]. The main compounds in the methanolic whole plant extract of VSM were recently identified as β-Sitosterol and Lupeol [ 29 ]. Both compounds were further tested for its effects on cell viability and cell migration of RMA cells (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…The stock solutions of plant-derived extracts were further diluted in different concentrations for the biological investigation and anti-tumor activity testing. The main secondary plant compounds of Vincetoxicum arnottianum (VSM) and Linum usitatissimum L. (LW) were previously identified by NMR and LC-MS analysis in accordance to their retention time [ 22 , 29 ] and listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton

Adamus

Ali

Vasileiadis

et al. 2021

BMC Complement Med Ther

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Background Prevention of metastatic invasion is one of the main challenges in the treatment of alveolar rhabdomyosarcoma. Still the therapeutic options are limited. Therefore, an anti-tumor screening was initiated focusing on the anti-metastatic and anti-invasion properties of selected medicinal plant extracts and phytoestrogens, already known to be effective in the prevention and treatment of different cancer entities. Methods Treatment effects were first evaluated by cell viability, migration, invasion, and colony forming assays on the alveolar rhabdomyosarcoma cell line RH-30 in comparison with healthy primary cells. Results Initial anti-tumor screenings of all substances analyzed in this study, identified the plant extract of Vincetoxicum arnottianum (VSM) as the most promising candidate, harboring the highest anti-metastatic potential. Those significant anti-motility properties were proven by a reduced ability for migration (60%), invasion (99%) and colony formation (61%) under 48 h exposure to 25 μg/ml VSM. The restricted motility features were due to an induction of the stabilization of the cytoskeleton – actin fibers were 2.5-fold longer and were spanning the entire cell. Decreased proliferation (PCNA, AMT, GCSH) and altered metastasis (e. g. SGPL1, CXCR4, stathmin) marker expression on transcript and protein level confirmed the significant lowered tumorigenicity under VSM treatment. Finally, significant alterations in the cell metabolism were detected for 25 metabolites, with levels of uracil, N-acetyl serine and propanoyl phosphate harboring the greatest alterations. Compared to the conventional therapy with cisplatin, VSM treated cells demonstrated a similar metabolic shutdown of the primary cell metabolism. Primary control cells were not affected by the VSM treatment. Conclusions This study revealed the VSM root extract as a potential, new migrastatic drug candidate for the putative treatment of pediatric alveolar rhabdomyosarcoma with actin filament stabilizing properties and accompanied by a marginal effect on the vitality of primary cells. Graphical abstract

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“…25,26 However, this is not the first report of the occurrence of nor-oleanane pentacyclic triterpenoids 27 28 in nature. Compounds (2), 21,22 (3), 29 and (4) 30,31 have been previously described (Figures S2 to S4). However, the ECD spectrum of compound ( 2) is presented for the first time, to the best of our knowledge.…”

Section: Spectrometric and Spectroscopic Study Of Isolated Compoundsmentioning

confidence: 99%

Pentacyclic Triterpenoids from Combretum platypetalum subsp. oatesii (Rolfe) Exell (Combretaceae) Root Inhibit Sterol 14α-Demethylase Target

2023

TJNPR

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Azole antifungal agents are sterol 14a-demethylase inhibitors. 4 This guided our search for antifungal agents from Combretum species used over the years in the treatment of fungal-related infections by African traditional healers. The bioguided isolation approach was used in the study of Combretum platypetalum subsp. oatesii (Rolfe) Exell (Combretaceae), overcoming the challenge of random isolation of inactive compounds, which has characterized most natural product drug discovery programs. The investigated species is a dwarf shrub peculiar to Western and Southern Africa. It is locally known as the red wing, which translates as adaidad mba in the Annang local dialect of Nigeria or motserwe in Setswana. In the African traditional medicine system, C. platypetalum is used in the treatment of several infectious and non-infectious diseases. 8 However, the plant part used in the traditional treatment is not explicitly stated in the literature. 8 The species' phytochemical investigation has identified three compounds from the leaves, 9, 10 but no compounds from its stem or root have been reported, except for the metabolomic identification of non-volatile bioactive constituents by Majinda and co-workers. 8 The literature survey reveals a diverse range of extraction solvents and methods used in studying the genus. 11 This paper aims to identify potential antifungal lead compounds from C. Platypetalum and for the first time, we describe the bioguided isolation of compounds (1-4) from Combretum platypetalum root. The study reports on the in vitro and computational antifungal activities of the isolated compounds, and their molecular docking for druggability against the sterol 14α-demethylase fungal target site and gives directions for future investigations.

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Anticancer Activity of Hispidulin from Saussurea simpsoniana and Lupeol from Vincetoxicum arnottianum

Cited by 3 publications

References 10 publications

Identification of Chemical Compounds in Plumbago zeylanica Linn Leaves from Indonesia

Identification of Chemical Compounds in Plumbago zeylanica Linn Leaves from Indonesia

Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton

Pentacyclic Triterpenoids from Combretum platypetalum subsp. oatesii (Rolfe) Exell (Combretaceae) Root Inhibit Sterol 14α-Demethylase Target

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