Anticancer activity of Vicenin‐2 against 7,12 dimethylbenz[<i>a</i>]anthracene‐induced buccal pouch carcinoma in hamsters

Li, Yijun; Zheng, Yi; Wang, Huibo

doi:10.1002/jbt.22673

Cited by 15 publications

(7 citation statements)

References 44 publications

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“…Moreover, this finding reflected on H&E stain that showed the classical epithelium mucosal layer. This finding was in consistence with that of other studies (28,29) . With regard to AgNOR stain, currant study recorded the mean dots number per nucleus in GI was 2.1.…”

Section: Discussionsupporting

confidence: 93%

chemopreventive efficacy of honokiol on experimentally induced hamster buccal pouch squamous cell carcinoma

Mosbah

Mahmoud²,

Abd-Alhafez³

et al. 2022

Al-Azhar Journal of Dental Science

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The aim of the present study was to investigate the effect of honokiol on induced hamster buccal pouch (HBP) squmase cell carcinoma (SCC). Subjects and methods: Thirty hamsters were used and divided into three group (s) (G (S) ), 10 each. GI (normal) was left untreated, the right HBP in GII were painted with 0.5% DMBA (3times/week/14weeks). GIII was treated as GII, in addition, they received honokiol. After termination of experiment, the hamsters were clinically examined, then, euthanized. After that, HBP was excised and routinely prepared for histological examination using hematoxylin and eosin (H&E) stain to record the histopathological findings and the depth of invasion, and histochemically examination utilizing AgNOR stain and picrosiures red stain as proliferative and invasive marker respectively, to record the mean area percentages for statistical analysis. Results: Gross and histopathological observations revealed variable changes in GIII compared to GII. The statistical analysis results, for the depth of invasion, revealed high significant difference between GII and GIII, with p-value:<0.001. Regarding the AgNOR stain, there was high significant difference between GI and GII and, also, between GII and GIII with p-value: <0.001. The polarizing colors of collagen fibers recorded significant difference between GI and GII, GI and GIII, GII and GIII with p-values: 0.042, 0.200, and: 0.175 respectively. The area fraction of the collagen fibers recorded high significant difference between GI and GII and, also, between GII and GIII with p-values: <0.001 for both. Conclusions: Honokiol significantly inhibited of invasion, proliferative activity and tumor progression in DMBA induced HBP SCCS.

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Section: Discussionsupporting

confidence: 93%

chemopreventive efficacy of honokiol on experimentally induced hamster buccal pouch squamous cell carcinoma

Mosbah

Mahmoud²,

Abd-Alhafez³

et al. 2022

Al-Azhar Journal of Dental Science

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“…but also by the interference of unidentified earlier compounds. For example, the pro-apoptotic effect of ExOm may be partially ascribed (except for RA-CA subfraction), to vicenin-2 (not detected in the remaining extracts) whose anti-cancer properties have been demonstrated in some studies ( Nagaprashantha et al, 2011 ; Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Modulatory Impact of Lamiaceae Metabolites on Apoptosis of Human Leukemia Cells

et al. 2022

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Lamiaceae species are rich sources of biologically active compounds which have been applied in medicine since ancient times. Especially their antineoplastic properties have been thoroughly studied with respect to their putative application in chemoprevention and adjuvant therapy of cancer. However, the most known biological effects of Lamiaceae have been ascribed to their essential oil fractions, whereas their (poly)phenolic metabolites being also abundant in these plants, are much less recognized, nevertheless contributing to their beneficial properties, such as anti-cancer actions. The aim of this study was to evaluate the impact of dried aqueous extracts from common thyme (Thymus vulgaris L.) (ExTv), wild thyme (Thymus serpyllum L.) (ExTs), sweet marjoram (Origanum majorana L.) (ExOm), and peppermint (Mentha × piperita L.) (ExMp), as well as (poly)phenolic compounds: caffeic acid (CA), rosmarinic acid (RA), lithospermic acid (LA), luteolin-7-O-β-glucuronide (Lgr), luteolin-7-O-rutinoside (Lr), eriodictyol-7-O-rutinoside (Er), and arbutin (Ab), on unstimulated Jurkat cells, in comparison with their effect on staurosporine-stimulated Jurkat cells. Jurkat T cells were incubated with different concentrations of ExTv, ExTs, ExOm, ExMp, Lgr, LA, Er, Lr, RA, CA, or Ab. Subsequently, staurosporine was added to half of the samples and flow cytometry combined with fluorescence-activated cell sorting analysis was conducted, which allowed for the selection of early and late apoptotic cells. Both ExTs and ExOm stimulated apoptosis of Jurkat cells and enhanced the proapoptotic effect of staurosporine. Conversely, ExTv and ExMp demonstrated no clear effect on apoptosis. CA and RA raised the staurosporine-induced apoptotic effect. The impact of Er and Lgr on Jurkat cells showed fluctuations depending on the compound concentration. Neither Er nor Ab altered staurosporine-induced apoptosis in Jurkat cells, whereas Lgr seemed to weaken the proapoptotic action of staurosporine. The most evident observation in this study was the pro-apoptotic action of ExTs and ExOm observed both in staurosporine-unstimulated and stimulated Jurkat cells. Additionally, an enhancement of staurosporine-induced apoptosis by caffeic and rosmarinic acids was reported. Therefore, it might be concluded that these are the mixtures of biologically active polyphenols which often exert more pronounced beneficial effects than purified molecules.

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“…The multiple effects of vicenin-2 ( 1 ) suggest its ability to modulate oncogenic, tumor suppressor, and differentiation cell signaling cascades to induce anticancer effects [ 53 ]. Recently, Li and colleagues described the in vivo effects of vicenin-2 ( 1 ; 30 mg kg −1 ), namely, the inhibition of Bcl-2 by triggering and/or activating apoptotic Bax in oral squamous cell carcinoma (OSCC) cell models [ 54 ]. Singhal and colleagues allowed us to know the synergistic effects of vicenin-2 ( 1 ), upon co-treatment with docetaxel in androgen-independent prostate cancer [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

Ribeiro

Ferreres

Oliveira

et al. 2023

Life

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The present work aimed to detail the mechanisms elicited by Allophylus africanus P. Beauv. stem bark extract in human stomach cancer cells and to identify the bioactives underlying the cytotoxicity. MTT reduction and LDH leakage assays allowed characterizing the cytotoxic effects in AGS cells, which were further detailed by morphological analysis using phalloidin and Hoechst 33258. Proapoptotic mechanisms were elucidated through a mitochondrial membrane potential assay and by assessing the impact upon the activity of caspase-9 and -3. The extract displayed selective cytotoxicity against AGS cells. The absence of plasma membrane permeabilization, along with apoptotic body formation, suggested that pro-apoptotic effects triggered cell death. Intrinsic apoptosis pathway activation was verified, as mitochondrial membrane potential decrease and activation of caspase-9 and -3 were observed. HPLC-DAD profiling enabled the identification of two apigenin-di-C-glycosides, vicenin-2 (1) and apigenin-6-C-hexoside-8-C-pentoside (3), as well as three mono-C-glycosides-O-glycosylated derivatives, apigenin-7-O-hexoside-8-C-hexoside (2), apigenin-8-C-(2-rhamnosyl)hexoside (4) and apigenin-6-C-(2-rhamnosyl)hexoside (5). Isovitexin-2″-O-rhamnoside (5) is the main constituent, accounting for nearly 40% of the total quantifiable flavonoid content. Our results allowed us to establish the relationship between the presence of vicenin-2 and other apigenin derivatives with the contribution to the cytotoxic effects on the presented AGS cells. Our findings attest the anticancer potential of A. africanus stem bark against gastric adenocarcinoma, calling for studies to develop herbal-based products and/or the use of apigenin derivatives in chemotherapeutic drug development.

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Anticancer activity of Vicenin‐2 against 7,12 dimethylbenz[a]anthracene‐induced buccal pouch carcinoma in hamsters

Cited by 15 publications

References 44 publications

chemopreventive efficacy of honokiol on experimentally induced hamster buccal pouch squamous cell carcinoma

chemopreventive efficacy of honokiol on experimentally induced hamster buccal pouch squamous cell carcinoma

Modulatory Impact of Lamiaceae Metabolites on Apoptosis of Human Leukemia Cells

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

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