Anticancer and antimicrobial activities of scorpion venoms and their peptides

Akef, Hassan M.

doi:10.1080/15569543.2017.1414847

Cited by 14 publications

(9 citation statements)

References 52 publications

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“…Finding a new effective drug to treat colon and breast cancer is greatly needed. Scorpion venom has a powerful antitumor effect and their compounds are cytotoxic to several cancer cell lines [ 15 , 50 , 51 , 52 ]. In this study, we showed that leptulipin inhibits the cell proliferation of MDA-MB-231 and HT-29 cancer cells and that it increases LDH cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Discovery of Leptulipin, a New Anticancer Protein from theIranian Scorpion, Hemiscorpius lepturus

et al. 2022

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Cancer is one of the leading causes of mortality in the world. Unfortunately, the present anticancer chemotherapeutics display high cytotoxicity. Accordingly, the discovery of new anticancer agents with lower side effects is highly necessitated. This study aimed to discover an anticancer compound from Hemiscorpius lepturus scorpion venom. Bioactivity-guided chromatography was performed to isolate an active compound against colon and breast cancer cell lines. 2D electrophoresis and MALDI-TOF were performed to identify the molecule. A partial protein sequence was obtained by mass spectrometry, while the full-length was deciphered using a cDNA library of the venom gland by bioinformatics analyses and was designated as leptulipin. The gene was cloned in pET-26b, expressed, and purified. The anticancer effect and mechanism action of leptulipin were evaluated by MTT, apoptosis, and cell cycle assays, as well as by gene expression analysis of apoptosis-related genes. The treated cells displayed inhibition of cell proliferation, altered morphology, DNA fragmentation, and cell cycle arrest. Furthermore, the treated cells showed a decrease in BCL-2 expression and an increase in Bax and Caspase 9 genes. In this study, we discovered a new anticancer protein from H. lepturus scorpion venom. Leptulipin showed significant anticancer activity against breast and colon cancer cell lines.

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Section: Discussionmentioning

confidence: 99%

Discovery of Leptulipin, a New Anticancer Protein from theIranian Scorpion, Hemiscorpius lepturus

et al. 2022

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“…It also exhibited the advantage of being able to penetrate deep into tumor tissue [217,218]. Since the discovery of CTx, the list of scorpion crude venoms and isolated toxins with anticancer activity has been growing rapidly, hence a comprehensive review of all reported compounds exceeds the scope of this review, but can be found elsewhere [216][217][218][219]. Here, we present a few cherry-picked recent studies with the most significant findings.…”

Section: Anticancer Effectsmentioning

confidence: 99%

“…CTx was the first scorpion-derived agent that demonstrated inhibitory effects on glioma cell migration and invasion. It also exhibited the advantage of being able to penetrate deep into tumor tissue [217,218]. Since the discovery of CTx, the list of scorpion crude venoms and isolated toxins with anticancer activity has been growing rapidly, hence a comprehensive review of all reported compounds exceeds the scope of this review, but can be found elsewhere [216][217][218][219].…”

Section: Anticancer Effectsmentioning

confidence: 99%

Scorpion Venom: Detriments and Benefits

et al. 2020

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Scorpion venom may cause severe medical complications and untimely death if injected into the human body. Neurotoxins are the main components of scorpion venom that are known to be responsible for the pathological manifestations of envenoming. Besides neurotoxins, a wide range of other bioactive molecules can be found in scorpion venoms. Advances in separation, characterization, and biotechnological approaches have enabled not only the development of more effective treatments against scorpion envenomings, but have also led to the discovery of several scorpion venom peptides with interesting therapeutic properties. Thus, scorpion venom may not only be a medical threat to human health, but could prove to be a valuable source of bioactive molecules that may serve as leads for the development of new therapies against current and emerging diseases. This review presents both the detrimental and beneficial properties of scorpion venom toxins and discusses the newest advances within the development of novel therapies against scorpion envenoming and the therapeutic perspectives for scorpion toxins in drug discovery.

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“…Scorpion venom is a mixture of peptide, protein, neurotoxin and salt. These venoms have been evaluated as potential therapeutics against many diseases such as cancer, viral and microbial from past to present [10].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species

Koç

Avşar

Bayrakcı

2020

Hacettepe Journal of Biology and Chemistry

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B u çalışmanın amacı, bazı akrep türlerinden elde edilen hemolenf ve zehirlerin antibakteriyal ve antifungal özelliklerinin belirlenmesidir. Zehir örnekleri elektrik stimülasyonu yöntemiyle beş farklı akrep türünden elde edilmiştir. Bununla birlikte hemolenf sıvısı da beş farklı akrep türünden elde edilmiştir. 1:1 oranında tuz solüsyonu ile seyreltilen hemolenf ve zehirlerin antimikrobiyal aktiviteleri yedi bakteri ve üç mayadan oluşan 10 mikroorganizmaya karşı disk difüzyon yöntemi ile test edilmiştir. Protoiurus kraepelini'den elde edilen zehir örnekleri test edilen bütün mikroorganizmalar üzerinde 9-20 mm inhibisyon zonu meydana getirmiştir. Bu türden elde edilen zehir Bacillus subtilis'e karşı en yüksek antibakteriyal aktivite gösteren örnek olmuştur. Mesobuthus gibbosus'dan alınan zehir örnekleri Enterococcus faecalis ve Micrococcus luteus haricindeki tüm mikroorganizmalarda etkili (7-12 mm) olmuştur. Ayrıca, Androctonus crassicauda, Hottentotta saulcyi ve Leiurus abdullahbayrami'den elde edilen zehirler farklı düzeylerde antibakteriyal etkiye sahip olmakla birlikte Candida türleri üzerinde antifungal bir etkisi olmamıştır. Akrep hemolenflerinin Gram-pozitif ve Gram-negatif bakteriler üzerinde benzer etkilerinin olduğu gösterilmiştir. Bunun yanında, elde edilen hemolenflerin mikroorganizmalar üzerinde düşük etkiye (7-9 mm) sahip olduğu tespit edilmiştir.

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Anticancer and antimicrobial activities of scorpion venoms and their peptides

Cited by 14 publications

References 52 publications

Discovery of Leptulipin, a New Anticancer Protein from theIranian Scorpion, Hemiscorpius lepturus

Discovery of Leptulipin, a New Anticancer Protein from theIranian Scorpion, Hemiscorpius lepturus

Scorpion Venom: Detriments and Benefits

Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species

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