Anticancer and Antioxidant Activities of the Root Extract of the Carnivorous Pitcher Plant Sarracenia purpurea

Huang, Yen‐Hua; Chiang, Wei-Yu; Chen, Pin-Jui; Lin, En‐Shyh; Huang, Cheng-Yang

doi:10.3390/plants11131668

Cited by 8 publications

(9 citation statements)

References 94 publications

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“…All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and were of analytical grade. The 4T1 mammary carcinoma [ 54 , 55 ] and B16F10 murine melanoma [ 56 , 57 , 58 ] cell lines were obtained from the Food Industry Research and Development Institute, Hsinchu, Taiwan. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and incubated at 37 °C in a humidified incubator with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

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Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Chen

Lin

et al. 2023

Plants

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Sinningia bullata is a tuberous member of the flowering plant family Gesneriaceae. Prior to this work, the antibacterial, antioxidant, and cytotoxic properties of S. bullata were undetermined. Here, we prepared different extracts from the leaf, stem, and tuber of S. bullata and investigated their pharmacological activities. The leaf extract of S. bullata, obtained by 100% acetone (Sb-L-A), had the highest total flavonoid content, antioxidation capacity, and cytotoxic and antibacterial activities. Sb-L-A displayed a broad range of antibacterial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The inhibition zones of Sb-L-A ranged from 8 to 30 mm and were in the following order: S. aureus > E. coli > P. aeruginosa. Incubation of B16F10 melanoma cells with Sb-L-A at a concentration of 80 μg/mL caused deaths at the rate of 96%, reduced migration by 100%, suppressed proliferation and colony formation by 99%, and induced apoptosis, which was observed in 96% of the B16F10 cells. In addition, the cytotoxic activities of Sb-L-A were synergistically enhanced when coacting with the antitumor drug epothilone B. Sb-L-A was also used to determine the cytotoxic effects against 4T1 mammary carcinoma cells. Sb-L-A of 60 μg/mL boosted the distribution of the G2 phase from 1.4% to 24.4% in the B16F10 cells. Accordingly, Sb-L-A might suppress melanoma cell proliferation by inducing G2 cell-cycle arrest. The most abundant compounds in Sb-L-A were identified using gas chromatography–mass spectrometry. The top two contents in this extract were adlupulone and villosin. Overall, the collective data in this study may indicate the pharmacological potentials of Sb-L-A for possible medical applications.

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Section: Methodsmentioning

confidence: 99%

“…Phytochemical components of Sb-L-A were determined by GC-MS. The filtered sample was analyzed using Thermo Scientific TRACE 1300 Gas Chromatograph with a Thermo Scientific ISQ Single Quadrupole Mass Spectrometer system (Waltham, MA, USA) [ 54 , 56 , 57 , 58 ]. The column used was Rxi-5ms (30 m × 0.25 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Chen

Lin

et al. 2023

Plants

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“…K. pneumoniae MGH 78578 [91][92][93][94] was used for the antibacterial assay. The cell lines PC-9 pulmonary adenocarcinoma, 4T1 carcinoma, and B16F10 murine melanoma were obtained from the Food Industry Research and Development Institute, Hsinchu, Taiwan [95].…”

Section: Chemicals Cell Lines and Bacterial Strainsmentioning

confidence: 99%

“…Phytochemical components of N. miranda-leaf-acetone were determined by GC-MS [95]. The filtered sample was analyzed using Thermo Scientific TRACE 1300 Gas Chromatograph with a Thermo Scientific ISQ Single Quadrupole Mass Spectrometer system.…”

Section: Gc-ms Analysismentioning

confidence: 99%

Cytotoxic Activities and the Allantoinase Inhibitory Effect of the Leaf Extract of the Carnivorous Pitcher Plant Nepenthes miranda

Lin

Huang

2022

Plants

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Nepenthes are carnivorous pitcher plants that have several ethnobotanical uses, such as curing stomachache and fever. Here, we prepared different extracts from the stem, leaf, and pitcher of Nepenthes miranda to further investigate their pharmacological potential. The leaf extract of N. miranda obtained by 100% acetone (N. miranda-leaf-acetone) was used in this study to analyze the cytotoxic activities, antioxidation capacity, antibacterial activity, and allantoinase (ALLase) inhibitory effect of this plant. The cytotoxic effects of N. miranda-leaf-acetone on the survival, apoptosis, and migration of the cancer cell lines PC-9 pulmonary adenocarcinoma, B16F10 melanoma, and 4T1 mammary carcinoma cells were demonstrated. Based on collective data, the cytotoxic activities of N. miranda-leaf-acetone followed the order: B16F10 > 4T1 > PC-9 cells. In addition, the cytotoxic activities of N. miranda-leaf-acetone were synergistically enhanced when co-acting with the clinical anticancer drug 5-fluorouracil. N. miranda-leaf-acetone could also inhibit the activity of ALLase, a key enzyme in the catabolism pathway for purine degradation. Through gas chromatography–mass spectrometry, the 16 most abundant ingredients in N. miranda-leaf-acetone were identified. The top six compounds in N. miranda-leaf-acetone, namely, plumbagin, lupenone, palmitic acid, stigmast-5-en-3-ol, neophytadiene, and citraconic anhydride, were docked to ALLase, and their docking scores were compared. The docking results suggested plumbagin and stigmast-5-en-3-ol as potential inhibitors of ALLase. Overall, these results may indicate the pharmacological potential of N. miranda for further medical applications.

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“…Betulin and α-amyrin have recently been found in a S. purpurea root extract which exhibits cytotoxicity towards the in vitro survival, migration and proliferation of mammary carcinoma cells with high tumorigenic and invasive potential [ 205 ]. Betulin and betulinic acid inhibit proliferation and induce apoptosis in various cancer cell lines such as breast [ 206 ], prostate [ 207 ], colorectal [ 208 ], leukemia [ 209 ] and lung [ 210 ].…”

Section: Triterpenesmentioning

confidence: 99%

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

Miclea

2022

IJMS

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Carnivorous plants have fascinated researchers and hobbyists for centuries because of their mode of nutrition which is unlike that of other plants. They are able to produce bioactive compounds used to attract, capture and digest prey but also as a defense mechanism against microorganisms and free radicals. The main purpose of this review is to provide an overview of the secondary metabolites with significant biological activity found in the Sarraceniaceae family. The review also underlines the necessity of future studies for the biochemical characterization of the less investigated species. Darlingtonia, Heliamphora and Sarracenia plants are rich in compounds with potential pharmaceutical and medical uses. These belong to several classes such as flavonoids, with flavonol glycosides being the most abundant, monoterpenes, triterpenes, sesquiterpenes, fatty acids, alkaloids and others. Some of them are well characterized in terms of chemical properties and biological activity and have widespread commercial applications. The review also discusses biological activity of whole extracts and commercially available products derived from Sarraceniaceae plants. In conclusion, this review underscores that Sarraceniaceae species contain numerous substances with the potential to advance health. Future perspectives should focus on the discovery of new molecules and increasing the production of known compounds using biotechnological methods.

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Anticancer and Antioxidant Activities of the Root Extract of the Carnivorous Pitcher Plant Sarracenia purpurea

Cited by 8 publications

References 94 publications

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Cytotoxic Activities and the Allantoinase Inhibitory Effect of the Leaf Extract of the Carnivorous Pitcher Plant Nepenthes miranda

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

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