Anticancer Effect of Black Raspberry and Apricot Juice against Colon Cancer Induced by Azoxymethane in Male Rats

Abstract

This study was carried out to evaluate the anticancer effect of black raspberry juice (RJ) and apricot juice (AJ) against colon cancer induced by Azoxymethane (AOM) in rats and to examine the possible mechanisms. Forty two Sprague Dawley male rats were used and randomly distributed into 6 equal groups (n=7). The 1 st group was negative control and each rat from the other 5 groups was given a single subcutaneous dose (15 mg/kg b.wt.) of AOM once weekly for 2 weeks to induce colon cancer. The 2 nd group was kept positive control and rats in the 3 rd , 4 th , 5 th and 6 th groups were orally given RJ and AJ at low (5%) and high (10%) concentrations, respectively, daily for 12 weeks. Serum colon cancer biomarkers [(tumor necrosis factor α-(TNF-α); nuclear factor-κappaB (NF-κB); interleukin1β (IL-1β) and interleukin and 6 (IL6)] were measured and histopathology of the colon was done to evaluate the anticancer effect. Lipid peroxidation and activities of antioxidant enzymes in colon tissues and content of 8-hydroxy-2-deoxyguanosine (8-OHdG) (apoptosis marker) in colon DNA were measured to examine the possible mechanisms. The results showed that oral administration of RJ and AJ inhibited AOM-induced elevations in serum colon cancer biomarkers levels, antagonized preneoplastic abbrent colon crypts induced by AOM, decreased lipid peroxidation; increased activities of antioxidant enzymes in colon tissues and reduced elevation of 8-OHdG content in colon DNA. These results denoted that RJ and AJ have an anticancer effect against colon cancer. The mechanistic studies demonstrated that RJ and AJ inhibited lipid peroxidation, increased antioxidant enzyme activities (antagonized oxidative stress) and induced of apoptosis. It can be concluded that intake of black raspberry and apricot as fruit salad or juice may be beneficial for patients who suffer from colon cancer.

Evaluation of Anti-cancer Effect of Different Concentrations of Black Raspberries Extract on Oral Squamous Cell Carcinoma Cell Line (In Vitro Study)

“…In the same context, Zhang et al (2011) (13) , Kresty et al (2016) (7) , and Ibrahim. (2017) (14) reported similar results in cervical cancer, esophageal cancer and colorectal cancer respectively. Moreover, decreasing the IC 50 value with increasing time of treatment from 24h to 72h time intervals indicates that the cytotoxic effect of BR against OSCC cell line could be efficiently exerted with the same efficacy either by a high dose of BR for limited time, or reduced BR dose for prolonged time.…”

Assessment of carboxymethyl chitosan loaded-amygdalin nanoparticles as biocompatible drug delivery vehicle for anti-tumor efficacy

“…found that amygdalin reduced tumor cell proliferation in prostate and bladder cancer cells, causing a considrable cell cycle regression [8,9]. Ibrahim et al, (2017) found that the apricot juice has an anticancer effect against colon cancer by reducing colon cancer biomarkers TNF-α, NF-κB, IL-1β and IL6 levels in the serum [10]. Arshi et al, (2019) observed that the amygdalin decreased growth and proliferation of stomach cancer (AGS), lung cancer (A549) and breast cancer (MCF7) through down-regulating the expression level of two antiapoptotic genes [11].…”