Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Abstract

Objective: To investigate the anticancer effect of Illicium verum against human breast cancer MCF-7 cell line.
 Methods: An experimental study was conducted in Multidisciplinary and Tissue Culture Laboratory, Aga Khan University in collaboration with Pharmacology Department of Bahria University Medical and Dental College, Karachi, Pakistan from January 2021 to June 2021. MCF-7 cells of Luminal-A breast cancer were seeded in 96-well plate and treated with I.verum methanol extract. After incubation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye was used for cell viability and cell proliferation assays to determine the number of dead and viable cells, and the absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) plate reader. In cell viability assay, different doses of I. verum methanol extract were used to treat the MCF-7 (0.25, 0.5, 1, 3, 6, 12, 25, and 50µg/ml) cells. For apoptosis analysis, the cells were processed with 4´, 6-diamidino-2-phenylindole fluorescent nuclear dye (DAPI) and were examined for fluorescence intensity and apoptotic cells. For cell proliferation assay and apoptosis the IC50 dose of 5.5µg/ml I. verum methanol extract was used.
 Results: The MCF-7 cells showed a significant reduction (p-value <0.01) in cell viability in the presence of all tested doses of I. verum methanol extract, except for the dose of 0.25µg/ml. The IC50 dose 5.5µg/ml of same extract also showed a significant reduction (p-value <0.01) in cell proliferation and apoptosis induction in MCF-7 cells.
 Conclusions: Illicium verum methanol extract possesses very potent anticancer action against MCF-7 cells through cytotoxicity, reduction, and inhibition of cancer cells and by inducing apoptosis.
 doi: https://doi.org/10.12669/pjms.39.1.6580
 How to cite this: Pahore AK, Khan S, Karim N. Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line. Pak J Med Sci. 2023;39(1):---------. doi: https://doi.org/10.12669/pjms.39.1.6580
 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gold-Aptamer-Modified Metal–Organic Framework Drug Delivery Nanosystems for Combined Photothermal/Chemotherapy of Cancer

“…MCF-7 cells were cultured with DMEM (containing 10% fetal bovine serum and 1% penicillin and streptomycin) in an incubator at 37 °C and under 5% CO 2 …”

“…MCF-7 cells were cultured with DMEM (containing 10% fetal bovine serum and 1% penicillin and streptomycin) in an incubator at 37 °C and under 5% CO 2 . 43 In Vitro Cell Uptake and Fluorescent Imaging. The MCF-7 cells were seeded into 6-well plates (1 × 10 6 cells/well) for 24 h. Then, the cells were incubated with DOX (10 μg/mL) and DOX/Au-Apt@ZIF-8 (based on the DOX content and concentration of 10 μg/ mL) for 2 and 6 h, respectively.…”

Spice-Derived Phenolic Compounds: Potential for Skin Cancer Prevention and Therapy

“…Additionally, it is a valuable source of shikimic acid which is an essential intermediate for the synthesis of the antiviral drug oseltamivir (Tamiflu ® ) [172]. In addition to the aforementioned health benefits, an anticancer effect of Illicium verum has been demonstrated in various in vitro and in vivo tumor models [178][179][180][181][182][183].…”

Bioactivity and antibacterial effect of star anise biosynthesized silver nanoparticles against Streptococcus mutans: an in vitro study

“…In addition to having antiviral properties, star anise has gastroprotective, antimicrobial, antifungal, anti-inflammatory, antioxidant, soothing, and spasmolytic effects [ 2 ]. Several studies have reported the medicinal uses of star anise, such as proven anticancer effects against breast cancer cells [ 3 ], treatment of several diseases of microbial origin [ 2 ], dental disease prevention [ 4 ], prevention of diabetes-associated complications [ 5 ], etc.…”