Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells

Abstract

Curcumin is a key polyphenolic curcuminoid extracted from the root of turmeric rhizome Curcuma longa Linn, which is a frequently used Chinese herb for the treatment of cancer. The aim of the present study was to investigate the mechanism of the inhibitory effects of curcumin on nude mice with lung cancer A549 cell subsets side population (SP) and non-SP (NSP) cells. BALB/c mice were subcutaneously injected with the tumor cells of A549 SP or NSP subsets consisting of 1x10 9 cells/l (0.2 ml in total). After 16 days of inoculation with A549, the mice were intraperitoneally injected with curcumin (100 mg/kg, 0.2 ml) once every other day, eight times in total. A series of assays were performed to detect the effects of curcumin on: i) Tumor weight and size; ii) Notch and hypoxia inducible factor 1 (HIF-1) mRNA expression by quantitative polymerase chain reaction; and iii) vascular endothelial growth factor (VEGF) and nuclear factor-κB (NF-κB) by immunohistochemistry. It was determined that curcumin decreased the tumor weight and size, downregulated the expression of Notch and HIF-1 mRNA and suppressed the VEGF and NF-κB expression. These results indicated that curcumin inhibited lung cancer growth through the regulation of angiogenesis mediated by VEGF signaling.

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

“…(2015) skin B. senegalensis Elkhateeb et al. (2019) breast, colon C. longa Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Coker-Gurkan et al. (2018) ; Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Zhao et al.…”

“…(2019) breast, colon C. longa Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Coker-Gurkan et al. (2018) ; Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Zhao et al. (2018) ; Frassová and Rudá -Kučerová, 2018 ; Arumai Selvan et al.…”

“…(2018) ; Luo et al. (2018) ; Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Wang et al. (2018) ; Muhammad et al.…”

Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways

“…Furthermore, a previous study revealed that curcumin liposome exerts antitumor effects by affecting proliferation- and apoptosis-related molecules in rabbits (47). In addition, curcumin inhibits lung cancer growth through the regulation of vascular endothelial growth factor signaling in mice (48). This study demonstrated that curcumin could, to a large extent, block migration, upregulate the expression of E-cadherin and suppress the expression levels of vimentin.…”

The Role of Curcumin in Cancer Treatment

“…Curcumin exhibits its therapeutic potential in lung cancer treatment by acting on the Wnt/β-catenin-dependent pathway in the human lung cancer cell line A549 [ 35 ]. Curcumin also suppresses the vascular endothelial growth factor (VEGF0 and NF-κB expression in the same cell line [ 36 ]. In addition, curcumin has been shown to inhibit the expression of enhancer of zeste homolog 2 (EZH2) in lung cancer cells, which was followed by a decrease in the expression of the Notch 1 gene [ 37 ].…”