Anticancer Effects of Residual Powder from Barley-<i>Shochu</i> Distillation Remnants against the Orthotopic Xenograft Mouse Models of Hepatocellular Carcinoma <i>in Vivo</i>

Abstract

Barely-Shochu is a traditional Japanese liquor distilled from fermented barley with Saccharomyces cerevisiae. Barely-Shochu distillation remnants (SDR) are by-products in the manufacturing process of barley-Shochu. We have already reported on valuable powder from Shochu distillation remnants (PSDR) including antioxidative compounds such as polyphenols. In this study, we investigated the therapeutic effects of barely-PSDR against orthotopic xenograft mouse models of hepatocellular carcinoma (HCC) in vivo. We constructed a mouse model of HCC by orthotopical inoculation of HepG2 cells into the liver of SCID mice. Barely-PSDR (2250 mg/kg) was orally treated once each day for 21 d after the inoculation of HepG2 cells. The livers were removed from anaesthetized mice after the treatment with barely-PSDR and fixed in formalin. The liver sections were analyzed by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) methods. Remarkably high reduction of tumorigenesis was obtained in the mouse models of HCC after the oral administration of barely-PSDR in vivo. Induction of apoptosis in the liver section on the mouse models treated with barely-PSDR was observed. Furthermore, prolonged survival was obtained. Thus, therapeutic effects of barely-PSDR without side effects on the orthotopic xenograft mouse models were revealed for the first time.

Coevolution between Cancer Activities and Food Structure of Human Being from Southwest China

“…Lunasin present in barley has been observed to prevent skin cancer, which could play an important role in the prevention of cancer in humans [ 50 ]. Remarkably high reduction of tumorigenesis and induction of apoptosis in the liver section were achieved in the mouse models with barley-Shochu distillation remnants [ 51 ]. Germinated barley foodstuff significantly increased the production of a tumor suppressor gene, which showed promising antineoplastic effects [ 52 ].…”

The Tumor Growth Inhibitory Effect of a Standardized Extract of Cultured <i>Lentinula edodes</i> Mycelia Using Patient Derived Xenograft Model

“…1) Thus, SCID-PDX model is a valuable tool for confirming pharmacological efficacy in non-clinical studies for the development of various drugs including anti-cancer agents and therapeutic research. 2) The immunodeficient mice that we mainly use for PDX, are originally from C.B17 SCID mice discovered by Bosma et al, Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, U.S.A. 3) This mouse strain was provided to Nomura by Bosma, and Nomura et al improved it at Osaka University. The improved SCID mouse is known as Super SCID mouse 4) due to the undetectable level of serum immunoglobulin G (IgG) and IgM by enzyme-linked immunosorbent assay (ELISA), and these characteristics make it suitable for xenotransplantation.…”