Anticancer efficacy of 6-thioguanine loaded chitosan nanoparticles with or without curcumin

Rajashekaraiah, Rashmi; Kumar, Pramod; Prakash, N.; Rao, G. Srinivasa; Devi, V. Rama; Metta, Muralidhar; Narayanaswamy, H.D.; Swamy, M. N. S.; Satyanarayan, K.; Rao, Suguna; Rathnamma, D; Sahadev, A; Sunilchandra, U; Santhosh, CR; Dhanalakshmi, H.; Kumar, Sandeep; Ruban, S. Wilfred; Kalmath, G. P.; Gomes, Ana; Kumar, Kishore; Govindappa, Prem Kumar

doi:10.1016/j.ijbiomac.2020.01.117

Cited by 27 publications

(29 citation statements)

References 51 publications

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“…These approaches include liposomal delivery, micelles, microspheres and metallic and polymeric based nanoparticles (see Fig. 2) [66][67][68][69][70][71][72][73][74][75][76][77].…”

Section: Nanomedicine Approachesmentioning

confidence: 99%

“…Slow release with 95% of TG released after 60 days. Significant amount of cytotoxicity 48 h after nanoparticle exposure, probably caused by intracellular uptake of TG nanoparticles by pinocytosis Rajashekarajah [ 68 ] India Polymer-based drug delivery TG Chitosan In vitro (PA-1 cells) Drug release pH 4.8 > pH 7.4 of TG nanoparticles after 48 h (91.4% vs 74.0%). IC 50 values of 5.8, 12.9 and 3.9 µM for TG, curcumin and TG nanoparticles Govindappa [ 70 ] India Polymer-based drug delivery MP Chitosan In vivo (Wistar rats) This study investigated the in vivo toxicity profile of MP-NPs and MP Ahmed [ 74 ] Egypt Polymer-based drug delivery MP Chitosan In vitro (MCF-7 cells) ↑ Cytotoxicity of MP NP compared to MP Decreased cell survival of 8% (3.1 µM) and 55% (6.2 µM) for MP and MP NP Wan [ 71 ] China Polymer-based drug delivery TG Dialdehyde sodium alginate In vitro (HL-60 cells) Drug release at pH 5.0 (98.6%), no drug release at pH 7.4 Qiu [ 69 ] China Polymer-based drug delivery MP Hyaluronic In vivo (nude mice) ↑ T 1/2 , ↑ reduction tumor volume for HA-MP compared to MP ( P < 0.01) Kumar [ 72 ] India Polymer-based drug delivery MP Chitosan In vivo (Wistar rats) ↑ C max , ↑ bioavailability, ↑ T 1/2 for MP-NP compared to MP.…”

Section: Strategies For Thiopurine Drug Deliverymentioning

confidence: 99%

“…The most studied polymer for thiopurine delivery is chitosan. Thiopurine-loaded chitosan nanoparticles have been studied preclinically for thioguanine, azathioprine and mercaptopurine [ 68 , 70 , 72 , 74 , 92 ]. Chitosan nanoparticles display a pH-dependent drug release profile due to the solubility of chitosan [ 93 ].…”

Section: Strategies For Thiopurine Drug Deliverymentioning

confidence: 99%

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Advances in Thiopurine Drug Delivery: The Current State-of-the-Art

Bayoumy

Crouwel²,

Chanda

et al. 2021

Eur J Drug Metab Pharmacokinet

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Thiopurines (mercaptopurine, azathioprine and thioguanine) are well-established maintenance treatments for a wide range of diseases such as leukemia, inflammatory bowel disease (IBD), systemic lupus erythematosus (SLE) and other inflammatory and autoimmune diseases in general. Worldwide, millions of patients are treated with thiopurines. The use of thiopurines has been limited because of off-target effects such as myelotoxicity and hepatotoxicity. Therefore, seeking methods to enhance target-based thiopurine-based treatment is relevant, combined with pharmacogenetic testing. Controlled-release formulations for thiopurines have been clinically tested and have shown promising outcomes in inflammatory bowel disease. Latest developments in nano-formulations for thiopurines have shown encouraging pre-clinical results, but further research and development are needed. This review provides an overview of novel drug delivery strategies for thiopurines, reviewing modified release formulations and with a focus on nano-based formulations.

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“…These approaches include liposomal delivery, micelles, microspheres and metallic and polymeric based nanoparticles (see Fig. 2) [66][67][68][69][70][71][72][73][74][75][76][77].…”

Section: Nanomedicine Approachesmentioning

confidence: 99%

Section: Strategies For Thiopurine Drug Deliverymentioning

confidence: 99%

Section: Strategies For Thiopurine Drug Deliverymentioning

confidence: 99%

See 1 more Smart Citation

Advances in Thiopurine Drug Delivery: The Current State-of-the-Art

Bayoumy

Crouwel²,

Chanda

et al. 2021

Eur J Drug Metab Pharmacokinet

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“…It can be seen that nanoparticles were more effective on lung cancer cell line than the free drug. So, nanoparticles get more potency than the free drug [22]. So, in this study, we basically developed the polymeric nanoparticles of curcumin and compared it cytotoxicity with its free from.…”

Section: Kinetic Modellingmentioning

confidence: 99%

Effect of Polymeric Nanoparticles of Curcumin on A549 Cell Line

Saikia¹,

LALHLENMAWIA²,

LALDINCHHANA³

et al. 2020

Int J Curr Pharm Sci

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Objective: Lung cancer cell line specially A549 is one of the most common malignant tumors. Curcumin is obtained from rhizomes of curcuma longa and does possess biological and pharmacological properties, among them anti-tumor activity is also one of the major findings. Here we try to developed a method for delivery of curcumin for cancer therapy. Methods: Due to low water solubility and bioavailability curcumin delivery designed as polymeric nanoparticles and prepared by emulsification homogenization method. Results: Characterization done by evaluating zeta size, potential, scanning electron microscopy. Release pattern also studied along with encapsulation and loading data. Nanoparticles were found to be spherical from morphological study and found to be below 200 nm. It does possess cytotoxic activity against lung cancer cell line and potent in comparison with its free from. Conclusion: We can conclude that polymeric nanoparticles are suitable form to delivery for antitumor activity and have suitable anticancer ctivity.

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“…6-thioguanine (6-TG) or thiopurine antimetabolite, as an analog of purine nucleosides, was first recognized as a health-promoting agent in the treatment of neoplastic conditions. This cytotoxic agent is extensively applied to treat disorders like acute leukemia, auto-immune disease, and inflammatory bowel disease [7,8]. Nevertheless, high doses of 6-TG are toxic and can lead to serious side effects, such as bone marrow depression, causes myelosuppression, gastrointestinal complications, and liver problems [6,9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical sensing platform for simultaneous detection of 6-mercaptopurine and 6-thioguanine using RGO-Cu2O/Fe2O3 modified screen-printed graphite electrode

Irannezhad

Seyed-Yazdi

Hekmatara

2021

J. Electrochem. Sci. Eng.

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A sensitive electrochemical sensor was developed using reduced graphene oxide RGO-Cu2O/Fe2O3 nanocomposite for 6-mercaptopurine detection based on a facile fabrication method. The surface morphology and structural composition of this nanocomposite was evaluated by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and Fourier transform infrared (FT-IR) spectroscopy. The screen-printed graphite electrode (SPGE) modified with RGO-Cu2O/Fe2O3 nanocomposite (RGO-Cu2O/Fe2O3/SPGE) indicated excellent electrochemical properties to detect 6-mercaptopurine. The linear dynamic range was estimated between 0.05 and 400.0 μM for 6-mercaptopurine detection, with a limit of detection of 0.03 μM. Also, RGO-Cu2O/Fe2O3/SPGE sensor showed good activity for simultaneous detection of 6-mercaptopurine and 6-thioguanine. In the coexistence system of 6-mercaptopurine and 6-thioguanine, two clear and well-isolated voltammetric peaks were obtained by differential pulse voltammetry (DPV). Additionally, the proposed sensor was examined for applicability by determining 6-mercaptopurine and 6-thioguanine in real samples, and the recovery in the range of 97.5-103.0 % was obtained.

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Anticancer efficacy of 6-thioguanine loaded chitosan nanoparticles with or without curcumin

Cited by 27 publications

References 51 publications

Advances in Thiopurine Drug Delivery: The Current State-of-the-Art

Advances in Thiopurine Drug Delivery: The Current State-of-the-Art

Effect of Polymeric Nanoparticles of Curcumin on A549 Cell Line

Electrochemical sensing platform for simultaneous detection of 6-mercaptopurine and 6-thioguanine using RGO-Cu2O/Fe2O3 modified screen-printed graphite electrode

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