Anticancer potential of benzothiazolic derivative (E)-2-((2-(benzo[d]thiazol-2-yl)hydrazono)methyl)-4-nitrophenol against melanoma cells

Abstract

Malignant melanoma is an important type of cancer worldwide due to its aggressiveness and poor survival rate. Significant efforts to understand the biology of melanoma and approaches to treat the advanced disease are focused on targeted gene inhibitors. Frequently mutated genes, such as NRAS, B-RAF and TP53, significantly exceed the frequency of mutations of other genes, emphasizing their importance for future targeted therapies. Considering the antitumor activity of benzothiazolic derivatives, this study aimed to demonstrate the action of benzothiazolic (E)-2-((2-(benzo[d]thiazol-2-yl)hydrazono)methyl)-4-nitrophenol (AFN01) against three established human melanoma cell lines that recapitulate the molecular landscape of the disease in terms of its genetic alterations and mutations, such as the TP53, NRAS and B-RAF genes. The results presented here indicate that AFN01, as a significant cytostatic and cytotoxic drug due to its induction of DNA fragmentation, causes single and double DNA strand breaks, consequently inhibiting cell proliferation, migration and invasion by promoting apoptosis. Our data suggest that AFN01 might be considered as a future therapeutic option for managing melanoma.

Retracted: Inhibiting the PI3K/Akt, NF-κB signalling pathways with syringic acid for attenuating the development of oral squamous cell carcinoma cells SCC131

“…Cell migration assay was achieved as used in the procedure by Vasconcelos et al . [ 22 ] SCC131 cells were seeded in 24‐well plate, and the cells permit cell adhesion until reaching 90% confluent monolayer. Afterwards, confluent monolayer scratched was through in the centre of wells with a sterile micropipette tip (10 μl).…”

Lignin Nanoparticles Deliver Novel Thymine Biomimetic Photo-Adducts with Antimelanoma Activity

“…In addition, LNPs/8a and LNPs/8b showed values of SI of the same order of magnitude than 8a and 8b, confirming the efficacy of LNPs in the drug delivery process. Note that the recorded SI values were higher than those, or of the same order of magnitude as, the approved drugs such as camptotechin, cisplatin, and doxorubicin [82,83]. Results in terms of IC 50 and SI after 24 h of treatment with compounds 4a-d, 6a-d, and 8a-d in a normal human fibroblast cell line (FB789) and two melanoma cell lines (SK-Mel28, RMPI7951).…”

Anticancer potential of corilagin on T24 and TSGH 8301 bladder cancer cells via the activation of apoptosis by the suppression of NF‐κB‐induced P13K/Akt signaling pathway

“…The wound and scratch healing assay was performed according to the procedure of Vasconcelos et al 18 T24 and TSGH 8301 cells were planted and permitted to adhere up to 90% confluent single coat was obtained. After that, a sterile 10 μl micropipette tip was routine to scrape the confluent monolayer in the midpoint of the well.…”