Anticancer Potential of Ficus religiosa (Linn) Against Human Breast Cancer Cell Line MDA-MB-231 and its Phytoconstituents by HR-LCMS

Abstract

Background: Ficus religiosa is widely present in India especially in northern region and has been traditionally used for various indications. We investigated the anticancer potential of leaf extract against human breast cancer cell line MDA-MB-231. The anticancer potential was further supported by identifying potential phytoconstituents of the extract by HR-LCMS analysis.Methods: Amongst the collected fractions, subfraction of chloroform was selected for anticancer screening in parallel for identification of phytochemicals by using HR-LCMS. Extract showed positive results in a battery of two tests employed in the current study by using MDA-MB231 breast cancer cell lines. In trypan blue exclusion dye test, 50% of cell killing was observed at 110 µg/ML. In vitro anticancer activity by using SRB assay showed dose dependent growth inhibition at 62.5, 125, 250 and 500 µg/mL concentrations. The parameters LC50, GI and TGI50 found from the assay were 100, 200, 300 µg/mL respectively. Assay parameter In case of HR-LCMS analysis several active phytochemicals were identified in the extract.Results: F. religiosa leaf extract showed promising anticancer activity against human breast cancer cell MDA-MB-231. Conclusion: F. religiosa leaf extract has promising anticancer potential against human breast cancer cells MDA-MB-231 and numerous phytochemicals further warrants evaluating it for multi-therapeutic potential.

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“… Medicinal Plants Phytochemicals Application Ref. Dicoma anomala Sesquiterpenes Breast, lung, prostate cancers [ 70 ] Aloe vera Aloe emodin, Emodin Head and neck cancer, glioblastoma, colon, breast cancer, and gastric carcinoma [ 63 , 71 ] Curcuma longa Curcumin Prostate, colorectal, breast, pancreatic, and head and neck cancers [ 72 , 73 ] Ipomoea mauritiana Scopoletin Lung, liver, skin and breast cancer [ 74 , 75 , 76 ] Berberis aristate Berberine Breast and colorectal cancer [ 77 , 78 ] Ficus religiosa Furanocoumarin Breast and ovarian cancer [ 79 , 80 ] Rubia cordifolia Rubiadin Human cervical, and larynx cancer [ 69 , …”