Anticancer Potential of L-Histidine-Capped Silver Nanoparticles against Human Cervical Cancer Cells (SiHA)

Abstract

This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of −20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.

Mollification of Doxorubicin (DOX)-Mediated Cardiotoxicity Using Conjugated Chitosan Nanoparticles with Supplementation of Propionic Acid

“…Antioxidants are the vital natural defense system of the body against free-radical-induced tissue damage [48]. The level of cellular antioxidants, as found in this study, confirms the status of oxidative stress-mediated tissue damage [49]. In the present study, the heart tissue recorded low levels of SOD and GST in DOX-and DCNPs+PA administered rats compared to untreated control [50].…”

Activities against Lung Cancer of Biosynthesized Silver Nanoparticles: A Review

“…The result can continue reacting with silver ions, lead to their reduction, and cause the formation of another complex [161]. In lung cancer treatment, the use of amino acids to synthesize AgNPs is limited; however, for other types of cancer, AgNPs synthesized with L-histidine can induce the apoptosis of SiHa cells (human cervical cancer cells) by inducing reactive oxygen species (ROS) and mitochondrial dysfunction [162].…”