Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment

Abstract

Thirty-two actinomycetes strains were isolated from sediment samples from 12 different sites at Lagos Lagoon and identified using standard physiological and biochemical procedures as well as 16S rDNA gene sequence analysis. Secondary metabolites were extracted from the strains and their anticancer activity on the K562 (Human acute myelocytic leukemia), HeLa (cervical carcinoma), AGS (Human gastric), MCF-7 (breast adenocarcinoma) and HL-60 (Human acute promyelocytic leukemia) cell lines was determined. The metabolic extracts exhibited cytotoxicity with IC 50 values ranging from 0.030 mg/mL to 4.4 mg/mL. The Streptomyces bingchenggensis ULS14 extract was cytotoxic against all the cell lines tested. The bioactivity-guided extraction and purification of the metabolic extracts from this strain yielded two purified anticancer compounds: ULDF4 and ULDF5. The structures of the extracted compounds were determined using spectroscopic analyses, including electrospray ionization mass spectrophotometer and nuclear magnetic resonance (1 Dimensional and 2 Dimensional), and were shown to be structurally similar to staurosporine and kigamicin. The IC 50 of ULDF4 and ULDF5 against the HeLa cell line was 0.034 μg/mL and 0.075 μg/mL, respectively. This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes.

Isolation and characterization of marine sponge–associated Streptomyces sp. NMF6 strain producing secondary metabolite(s) possessing antimicrobial, antioxidant, anticancer, and antiviral activities

“…Cancer is still one of the most life-threatening diseases worldwide. An estimated 10 million cancer-related deaths among 18 million cases diagnosed with cancer were reported in 2018, which is expected to increase to 26.4 million annual cases worldwide, with 17 million deaths by the year 2030 [ 3 , 4 ]. Oxidative stress is associated with the development of several pathologies such as cancer, Alzheimer’s disease, cardiovascular disease, early aging, ischemia, liver injury, arteriosclerosis, inflammation, skin damages, diabetes mellitus, and arthritis.…”

Marine Actinomycetes, New Sources of Biotechnological Products

“…Two examples of novel anticancer metabolites are the compound extracts ULDF4 and ULDF5 derived from Streptomyces strains found at Lagos. ULDF4 and ULDF5 exhibit cytotoxicity against human acute myelocytic leukemia, cervical carcinoma, human gastric carcinoma, breast adenocarcinoma, and human acute promyelocytic leukemia [ 129 ]. ULDF4 and ULDF5 are structurally similar to staurosporine and kigamicin, compounds known for inducing apoptosis and necrosis, respectively.…”

Presence of Biosynthetic Gene Clusters (NRPS/PKS) in Actinomycetes of Mangrove Sediment in Semarang and Karimunjawa, Indonesia

“…Samples were inserted into sterile ziplock bags, then dried at 26-32°C for two weeks in the laboratory. The treatment method used to isolate actinomycetes from the sediment samples was a modification of the method of Davies-Bolorunduro et al (2019). Isolation of actinomycetes was done using spread plate method by serial dilution.…”