Anticancer potential of nanogold conjugated toxin GNP-NN-32 from Naja naja venom

Abstract

Background: Cancer is the second most common fatal disease in the world, behind cardiovascular disorders in the first place. It accounts for around 0.3 million deaths per year in India due to the lack of proper diagnostic facilities, prevention and treatment. Current therapeutic methods do not provide adequate protection and affect normal cells along with cancerous ones. Thus, there is a need for some alternative therapeutic strategy, preferably from natural products, which have been traditionally used for treatment of various diseases in the country. Methods: In this study, we have conjugated purified NN-32 toxin from Naja naja venom with gold nanoparticles and its anticancer potential was evaluated against human breast cancer cell lines. UV-Vis spectroscopy, dynamic light scattering, transmission electron microscopy, atomic force microscopy and zeta potential analysis were the techniques used for characterization of GNP-NN-32. Results: GNP-NN-32 showed dose-and time-dependent cytotoxicity against breast cancer cell lines (MCF-7 and MDA-MB-231). NN-32 and GNP-NN-32 induced apoptosis in both breast cancer cell lines. The results of CFSE cell proliferation study revealed that NN-32 and GNP-NN-32 arrested cell division in both MCF-7 and MDA-MB-231 cell lines resulting in inhibition of proliferation of these cancer cells. Conclusion: GNP-NN-32 showed an anticancer potential against human breast cancer cell lines. Analysis of detailed chemical characterization along with its cytotoxic property might help to perceive a new dimension of the anti-cancer potential of GNP-NN-32 that will enhance its biomedical function in near future.

Recent Advances in Metal Decorated Nanomaterials and Their Various Biological Applications: A Review

“…The growing popularity of CuS nanoparticles in the field of biosensing is primarily based on conductivity and capacity of these nanoparticles, which encourage electron transfer reactions with molecules. Furthermore, CuS thin filmbased immunosensor was introduced for the exposure of anthropological immunoglobulin A (IgA) antibody in serum, in which a goat antihuman IgA antimediator was immobilized on surface of CuS thin film (Attarde and Pandit, 2020). The functionalized quantum dots of Ag 2 S are also important to use in bioimaging and diagnostics purposes.…”

Pharmacological Role of Functionalized Gold Nanoparticles in Disease Applications

“…Pegylated gold nanoparticles (PEGAuNPs, 24 nm, 9.8 nM) combined with chemotherapy drugs (doxorubicin or varlitinib) also have a very good cytotoxic effect on pancreatic cancer cells [ 49 ]. Toxin from Naja naja venom (NN-32, IC50: 5.0 µg/mL) combined with gold nanoparticles (18 nm) increased the percentage of cytotoxic activity and apoptosis of two human breast cancer cell lines (MCF-7 and MDA-MB-231) [ 50 ].…”

Compositional and toxicological investigation of pooled venom from farm-raised Naja atra

“…Several previous studies have reported the occurrence of muscle fiber degeneration after the injection of cardiotoxin from N. atra [44][45][46], which is similar to the pathological analysis results of heart tissue obtained in the present study. The study of Naja venom is important due to its great potential for medical application as an anticancer among others [47].…”