Anticancer Properties of Eisenia Foetida Proteins in Prostate Cancer Cells In Vitro

Özkan, Asuman Deveci; Eskiler, Gamze Güney; Sarıhan, Mehmet; Kazan, Nur; Aksoy, Özlem; Yüksel, Burcu; Betts, Zeynep

doi:10.1007/s10989-022-10428-8

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…Earthworm fibrinolytic enzymes (EFE) have been widely reported as potential therapeutic agents. EFEs could be extracted from various species of earthworms, such as Lumbricus rubellus [ 6 – 9 ], Eisenia fetida [ 10 – 12 ], E. andrei [ 13 ], and Perionyx excavatus [ 14 ]. These enzymes have potential application as active pharmaceutical ingredients (APIs) for the treatment of severe diseases such as heart or cerebral infarction and prevention of thrombus formation in surgery.…”

Section: Introductionmentioning

confidence: 99%

Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Stephani¹,

Rahayu²,

Retnoningrum

et al. 2023

Proteome Sci

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Background Lumbrokinase derived from earthworms, Lumbricus rubellus is known to have fibrinolytic enzymes that have potential as therapeutic drugs due to its ability to dissolve fibrin. The current study is aimed to purify the Lumbrokinase from L. rubellus and identify its protein component. Methods Water extract of local earthworm Lumbricus rubellus revealed several proteins. Therefore, to identify its protein component, purification through HiPrep DEAE fast flow and proteomic analysis were conducted prior to identifications. A combination of two-dimension gel electrophoresis (2DE) and electrospray ionization mass spectrometry analysis was used to identify the purified fractions. Results The purified fractions contain five protein bands, namely F25-1, F25-2, F85-1, F85-2, and F85-3, which displayed strong fibrinogenolytic activity. F25 fractions showed fibrinogenolytic activity of 974.85 U/mg, while F85 fractions showed higher activity of 1,484.11 U/mg. Fractions F85-1, F85-2, and F85-3 showed molecular weights of 42.6 kDa, 27.03 kDa, and 14 kDa, respectively and were identified as Lumbrokinase iso-enzymes. Conclusion This preliminary study indicates that the F25 and F85 fractions are similar to published fibrinolytic protease-1 and lumbrokinase, respectively, in terms of their amino acid sequence.

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Section: Introductionmentioning

confidence: 99%

Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Stephani¹,

Rahayu²,

Retnoningrum

et al. 2023

Proteome Sci

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“…Studies have found that the substances from E. foetida have multiple biological activities including anticancer and hepatoprotection . The lumbrokinase from earthworm significantly attenuated fibroblast migration and adhesion .…”

Section: Introductionmentioning

confidence: 99%

Promoted Skin Wound Healing by Tail-AmputatedEisenia foetidaProteins via the Ras/Raf/MEK/ERK Signaling Pathway

Sun

Wang

et al. 2023

ACS Omega

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Skin wound healing is an important fundamental problem in biological and medical fields. This study aimed to investigate wound healing promotion of protein extract from tail-amputated Eisenia foetida (E. foetida) and reveal the mechanism correlated with the Ras/Raf/MEK/ERK signaling pathway. Proteins extracted from tail-amputated E. foetida were applied on rats’ full-thickness excisional wounds to evaluate their regenerative efficacy. Rat skin tissues around surgical defects were analyzed by immunofluorescence staining and Western blot methods. The Ras/Raf/MEK/ERK signaling pathway was further investigated in vitro using the NIH3T3 cell line. A tail-amputated protein extract (ES2) from E. foetida significantly accelerated rat wound healing ability via higher re-epithelialization and ECM deposition in the tissue section compared to the blank control and un-amputated earthworm extract groups. Furthermore, ES2 treatment dramatically accumulated the expressions of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), and hydroxyproline (HYP) in wound areas on day 7 without their accumulation on day 21 post-wounding, diminishing excessive scar formation. Accelerated wound healing ability with the ES2 was proved to correlate with the up-regulation of the Ras/Raf/MEK/ERK signaling pathway. The mRNA expression of this pathway increased significantly in NIH3T3 cells after being treated with the ES2 at an appropriate concentration. The tail-amputated E. foetida proteins (ES2) can significantly promote skin wound healing better than the un-amputated earthworm tissue extract without excessive scar tissue formation. This effect was related to the up-regulation of the Ras/Raf/MEK/ERK signaling pathway.

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Protective effect of Eisenia foetida coelomic fluid against oxidative damage in human endothelial cells

Kilciler

Özkan

Betts

2022

Toxicological & Environmental Chemistry

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Anticancer Properties of Eisenia Foetida Proteins in Prostate Cancer Cells In Vitro

Cited by 7 publications

References 32 publications

Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Purification and proteomic analysis of potent fibrinolytic enzymes extracted from Lumbricus rubellus

Promoted Skin Wound Healing by Tail-AmputatedEisenia foetidaProteins via the Ras/Raf/MEK/ERK Signaling Pathway

Protective effect of Eisenia foetida coelomic fluid against oxidative damage in human endothelial cells

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