Anticlastogenic, antimutagenic, and cytoprotective properties of<i>Orthosiphon stamineus</i>ethanolic leaves extract

Al-Dulaimi, Dhamraa W.; Majid, Aman Shah Abdul; Baharetha, Hussein M.; Ahamed, Mohamed B. Khadeer; Faisal, Fahad; Zarzour, Raghdaa Hamdan Al; Oon, Chern Ein; Majid, Amin Malik Shah Abdul; Hassan, Loiy Elsir Ahmed

doi:10.1080/01480545.2020.1749652

Cited by 7 publications

(4 citation statements)

References 39 publications

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“…Orthosiphon aristatus is an important herbal medicine, with more than 20 phenolic compounds detected, including caffeic acid, rosmarinic acid, sinensetin, eupatorium, and polymethoxyflavones 12,24 . The beneficial effects of phenolic compounds in Orthosiphon aristatus include antioxidant 25 , antibacterial 26 , antifungal 27 , antidiabetic 28 , anti-inflammatory 15 , antimutagenic 29 , and anti-arthritic properties 30 . A study using a 200µg extract showed inhibitory effects on TPA (tetradecanoylphorbol)-induced inflammation in rats 31 .…”

Section: Discussionmentioning

confidence: 99%

The Potential of Orthosiphon Aristatus Extract in Improving Skin Lesions in Atopic Dermatitis: A Mice Model Study

Pandaleke,

Handono,

Widasmara

et al. 2024

Pharmacogn J.

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Introduction: This research discusses the potential of Orthosiphon aristatus, a medicinal plant, in improving skin lesions in Atopic Dermatitis by regulating Th2 cytokines and showing anti-allergic activity. Methods: The study was conducted using BALB/C mice induced with DNCB for 21 days to create an atopic dermatitis model. Then, the mice were orally administered Orthosiphon aristatus extract for 14 days (after 7 days of induction). The doses given were divided into six groups: 17.5 mg/kgbw, 35 mg/kgbw, 70 mg/kgbw, and 140 mg/kgbw. Molecular levels such as IgE, IL4, IL22, and PGE2 were analyzed from blood samples. In addition, the severity of skin lesions was assessed morphologically, and histological examination was performed to confirm clinical improvement. Results: This study showed that the administration of Orthosiphon aristatus extract reduced the severity of skin lesions in all intervention groups. Histologically, this extract reduced epidermal thickness and mast cell infiltration. These findings were also supported by dose-dependent decreases in IgE, IL4, IL22, and PGE2 levels. So, the conclusion of this study is that Orthosiphon aristatus extract shows potential for improving skin lesions in atopic dermatitis by regulating Th2 cytokines and demonstrating anti-allergic activity in a mice model with DNCBinduced atopic dermatitis. Conclusion; The administration of Orthosiphon aristatus extract clinically and histologically exhibits anti-atopic effects that can be explained through the regulation of molecules such as IgE, IL4, IL22, PGE2, and NO. Orthosiphon aristatus has the potential to be a valuable herbal therapy for managing atopic dermatitis.

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Section: Discussionmentioning

confidence: 99%

The Potential of Orthosiphon Aristatus Extract in Improving Skin Lesions in Atopic Dermatitis: A Mice Model Study

Pandaleke,

Handono,

Widasmara

et al. 2024

Pharmacogn J.

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“…Bene cial effects of phenolic compounds in Orthosiphon aristatus includes antioxidant [27], antibacterial [28], antifungal [29], antidiabetic [30], antiin ammatory [17], antimutagenic [31], and antiarthritic [32].…”

Section: Discussionmentioning

confidence: 99%

Orthosiphon aristatus Alleviates Skin Barrier through Cytokines Regulation in DNCB- induced Atopic Dermatitis BALB/C Mice Model

Handono

Widasmara²,

Susianti

et al. 2023

Preprint

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Background: to analysed the effect of Orthosiphon aristatus on repairing skin lesions by regulating cytokines such as IgE, IL4, IL22, PGE2, NO in DNCB-induced Atopic Dermatitis BALB/C Mice Model Methods: used BALB/C Mice which were sensitized by DNCB for 21 days to developed atopic dermatitis model. Mice were administered oral Orthosiphon aristatus extract once daily (on last 14 days after 7 days of sensitization). The doses given were divided into 6 groups: 17.5 mg/kgbw, 35 mg/kgbw, 70 mg/kgbw, and 140 mg/kgbw. We analysed the levels of cytokines such as IgE, IL4, IL22, PGE2 obtained from the blood. Additionally, we also measured morphological skin lesion severity to confirmed the amelioration effect clinically. Results: Administration of Orthosiphon aristatus extract reduced the skin lesion severity in all intervention groups. The clinical improvement was supported by decrease of IgE, IL4, IL22, and PGE2 in dose dependent manner. Conclussion: Orthosiphon aristatus alleviates DNCB-induced Atopic Dermatitis BALB/C Mice Model clinically through downregulating of IgE, IL4, IL22, PGE2, NO levels.

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“…In past decades, accumulated researches have evaluated the antineoplastic effects of O. aristatus in vivo and in vitro, and yet O. aristatus rarely used for neoplasm in traditional medicine. these researches showed that the standardized extracts of O. aristatus could inhibit the growth of hepatocellular carcinoma, fibrosarcoma, and colorectal carcinoma cell line by inducing apoptosis(Doleckova et al, 2012; Ahamed et al, 2012; Awale et al, 2002) or antimutagenic efficacies(Al-Dulaimi et al, 2020). The chloroform extract of O. aristatus had solid inhibitory activities against five cancer cell lines, and Luo et al .…”

Section: Discussionmentioning

confidence: 99%

“…In past decades, accumulated researches have evaluated the antineoplastic effects of O. aristatus in vivo and in vitro, and yet O. aristatus rarely used for neoplasm in traditional medicine. these researches showed that the standardized extracts of O. aristatus could inhibit the growth of hepatocellular carcinoma, fibrosarcoma, and colorectal carcinoma cell line by inducing apoptosis 6,7,48 or antimutagenic efficacies 49 . The chloroform extract of O. aristatus had solid inhibitory activities against five cancer cell lines, and Luo et al 50 concluded that the backbones of diterpenoids had a significant influence on cell toxicity due to the six active compounds (Spicatusene B, Spicatusene C, Orthosiphol R, Orthosiphol N, Orthoarisin A, Orthosiphonone D).…”

Section: Other Conditionsmentioning

confidence: 99%

An integrative analysis to predict the Active Ingredients and explore Polypharmacological Mechanisms of Orthosiphon stamineus Benth. for human health

Wang

Zhao

Zhang

et al. 2021

Preprint

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Background: Orthosiphon stamineus Benth. (OS) was a traditional folk herb with widespread clinical application, but it was lack of comprehensive understanding of its active ingredients and polypharmacological mechanisms. The aim of this work was to systematically study its natural compounds and the molecular mechanisms of the OS via network pharmacology. Methods: Compounds from OS were collected by literature retrieval and evaluated by SwissADME with the physicochemical property (ADMET model) and the likelihood for a natural medicine. The connection of active ingredients and target genes was built and confirmed by Cytoscape and AutoDock vina. Then, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were separately performed to obtain more in-depth understanding of OS. Finally, the relationship among active ingredients, targets, and diseases was built to clarify the polypharmacological mechanisms and found relevant active substances for further drug discovery. Results: A total of 140 compounds of OS were collected, and 16 potentially active ingredients were screened out. The Ingredient-Target Interaction Network was built with 6 flavonoids, 3 diterpenes, 3 phenols, 4 volatile oils, and 64 targets. The Docking analysis indicated that the ingredient-target interaction network was reliable; most ligand-receptor had a strong binding affinity (lowest binding energy: -6.9 kcal/mol). After pathway analysis, 185 significant biological processes and 36 signal pathways were found, and the ingredient-target-disease network of OS was constructed for polypharmacological mechanisms. Conclusion: Our study clarified the polypharmacological mechanisms via the relationship among active ingredients, targets, and diseases and provided better guidance for subsequent experiments and potential active ingredients for drug discovery.

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Anticlastogenic, antimutagenic, and cytoprotective properties ofOrthosiphon stamineusethanolic leaves extract

Cited by 7 publications

References 39 publications

The Potential of Orthosiphon Aristatus Extract in Improving Skin Lesions in Atopic Dermatitis: A Mice Model Study

The Potential of Orthosiphon Aristatus Extract in Improving Skin Lesions in Atopic Dermatitis: A Mice Model Study

Orthosiphon aristatus Alleviates Skin Barrier through Cytokines Regulation in DNCB- induced Atopic Dermatitis BALB/C Mice Model

An integrative analysis to predict the Active Ingredients and explore Polypharmacological Mechanisms of Orthosiphon stamineus Benth. for human health

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