Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

Zhao, Jun; Yang, Jin; Song, Shuang; Zhou, Da‐Yong; Qiao, Weizhou; C, Zhu; Liu, Shuyin; Zhu, Beiwei

doi:10.3390/molecules21060697

Cited by 20 publications

(8 citation statements)

References 23 publications

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“…In addition, Zhao et al . () also indicated that the polysaccharide from Haliotis discus hannai Ino with a high molecular weight demonstrated to possess a greater anticoagulant activity. Consequently, the reasons that TPS–FJ exhibited significantly ( P < 0.05) higher immunomodulatory activity than TPS–SC mainly might due to (i) the higher molecular weight when compared with TPS–SC; (ii) TPS–FJ exhibited triple‐helix conformation; (iii) TPS–FJ comprised a higher molar proportion of mannose and glucose than that of TPS–SC.…”

Section: Resultsmentioning

confidence: 97%

“…Furthermore, polysaccharides containing mannose and glucose were most likely recognised by pattern recognition receptors (Liao et al, 2015), and a high molar proportion of mannose and glucose may contribute to the enhancement of immunomodulatory activity (Hui et al, 2011). In addition, Zhao et al (2016) also indicated that the polysaccharide from Haliotis discus hannai Ino with a high molecular weight demonstrated to possess a greater anticoagulant activity. Consequently, the reasons that TPS-FJ exhibited significantly (P < 0.05) higher immunomodulatory activity than TPS-SC mainly might due to (i) the higher molecular weight when compared with TPS-SC; (ii) TPS-FJ exhibited triple-helix conformation; (iii) TPS-FJ comprised a higher molar proportion of mannose and glucose than that of TPS-SC.…”

Section: Immuno-stimulating Activities Of Tps-fj and Tps-sc In Vitromentioning

confidence: 97%

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Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Pan

Sun

Wang

et al. 2018

Int J of Food Sci Tech

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Summary In this study, we compared the chemical structure, antioxidant and immuno‐stimulating activities of two polysaccharides, TPS–FJ and TPS–SC, extracted from Tremella fuciformis from two of the major cultivation regions of China. TPS–FJ and TPS–SC contained protein and high uronic acid. Average molecular weights of TPS–FJ and TPS–SC were 45 461 and 25 981 kDa, respectively. A triple‐helix conformation was exhibited by TPS–FJ but not by TPS–SC. TPS–FJ and TPS–SC contained same monosaccharides but in different ratios. Both the polysaccharides displayed characteristic polysaccharide bands in their Fourier transform infrared and NMR spectra, shown high water and oil holding capacity, and retained antioxidant potential after simulated gastrointestinal digestion in vitro. TPS–FJ significantly (P < 0.05) stimulated the nitric oxide, tumour necrosis factor‐α and interleukin‐6 production of RAW 264.7 cells with higher reactions than those of TPS–SC in vitro. Thus, Tremella fuciformis polysaccharide can be used as a potential antioxidant and immunomodulatory ingredient in food industry.

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Section: Resultsmentioning

confidence: 97%

Section: Immuno-stimulating Activities Of Tps-fj and Tps-sc In Vitromentioning

confidence: 97%

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Pan

Sun

Wang

et al. 2018

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…The content of sulfate group is significantly different with different extraction methods and sources, which leads to significant differences in the activity of MSPs from different sources or even the same source. MSPs from abalone have a variety of fragments, and their activity is related to molecular weight and sulfate content [92,93]. A fraction named AGP33 with sulfates occur at 3-O-and 4-O-positions showed a better anticoagulant activity than its desulfated product [93].…”

Section: Role Of Sulfate Group and Sulphation Patternmentioning

confidence: 99%

“…MSPs from abalone have a variety of fragments, and their activity is related to molecular weight and sulfate content [92,93]. A fraction named AGP33 with sulfates occur at 3-O-and 4-O-positions showed a better anticoagulant activity than its desulfated product [93]. MSPs from abalone viscera also exhibited significantly hypolipidemic and anti-atherogenic activities [52].…”

Section: Role Of Sulfate Group and Sulphation Patternmentioning

confidence: 99%

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

et al. 2021

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Metabolic syndrome is the pathological basis of cardiovascular and cerebrovascular diseases and type 2 diabetes. With the prevalence of modern lifestyles, the incidence of metabolic syndrome has risen rapidly. In recent years, marine sulfate polysaccharides (MSPs) have shown positive effects in the prevention and treatment of metabolic syndrome, and they mainly come from seaweeds and marine animals. MSPs are rich in sulfate and have stronger biological activity compared with terrestrial polysaccharides. MSPs can alleviate metabolic syndrome by regulating glucose metabolism and lipid metabolism. In addition, MSPs prevent and treat metabolic syndrome by interacting with gut microbiota. MSPs can be degraded by gut microbes to produce metabolites such as short chain fatty acids (SCFAs) and free sulfate and affect the composition of gut microbiota. The difference between MSPs and other polysaccharides lies in the sulfation pattern and sulfate content, therefore, which is very important for anti-metabolic syndrome activity of MSPs. This review summarizes the latest findings on effects of MSPs on metabolic syndrome, mechanisms of MSPs in treatment/prevention of metabolic syndrome, interactions between MSPs and gut microbiota, and the role of sulfate group and sulfation pattern in MSPs activity. However, more clinical trials are needed to confirm the potential preventive and therapeutic effects on human body. It may be a better choice to develop new functional foods containing MSPs for dietary intervention in metabolic syndrome.

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“…For example, the EPS from Lactococcus lactis F-mou has similar anticlotting activity [ 98 ]. Normally, the structural composition, configuration, molecular weight, branching structure, and sulfate groups influence the anticoagulant activities of the EPS [ 99 ]. These in vitro anticoagulant effects of VITP14 EPS are most likely to have occurred due to the electrostatic interactions and their binding ability to coagulation factors of plasma.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Andrew

Jayaraman

2022

Polymers

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The use of natural polysaccharides as biomaterials is gaining importance in tissue engineering due to their inherent biocompatibility. In this direction, the present study aims to explore the structure and biocompatibility of the EPS produced by Virgibacillus dokdonensis VITP14. This marine bacterium produces 17.3 g/L of EPS at 96 h of fermentation. The EPS was purified using ion exchange and gel permeation chromatographic methods. The porous web-like structure and elemental composition (C, O, Na, Mg, P, S) of the EPS were inferred from SEM and EDX analysis. AFM analysis revealed spike-like lumps with a surface roughness of 84.85 nm. The zeta potential value of −10 mV indicates the anionic nature of the EPS. Initial molecular characterization showed that the EPS is a heteropolysaccharide composed of glucose (25.8%), ribose (18.6%), fructose (31.5%), and xylose (24%), which are the monosaccharide units in the HPLC analysis. The FTIR spectrum indicates the presence of functional groups/bonds typical of EPSs (O-H, C-H, C-O-H, C-O, S=O, and P=O). The polymer has an average molecular weight of 555 kDa. Further, NMR analysis revealed the monomer composition, the existence of two α- and six β-glycosidic linkages, and the branched repeating unit as → 1)[α-D-Xylp-(1 → 2)-α-D-Glcp-(1 → 6)-β-D-Glcp-(1 → 5)]-β-D-Frup-(2 → 2)[β-D-Xylp-(1 → 4)]-β-D-Xylp-(1 → 6)-β-D-Fruf-(2 → 4)-β-D-Ribp-(1 →. The EPS is thermally stable till 251.4 °C. X-ray diffraction analysis confirmed the semicrystalline (54.2%) nature of the EPS. Further, the EPS exhibits significant water solubility (76.5%), water-holding capacity (266.8%), emulsifying index (66.8%), hemocompatibility (erythrocyte protection > 87%), and cytocompatibility (cell viability > 80% on RAW264.7 and keratinocyte HaCaT cells) at higher concentrations and prolongs coagulation time in APTT and PT tests. Our research unveils the significant biocompatibility of VITP14 EPS for synthesizing a variety of biomaterials.

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Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

Cited by 20 publications

References 23 publications

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Comparison of structural, antioxidant and immuno‐stimulating activities of polysaccharides from Tremella fuciformis in two different regions of China

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

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