Anticoagulant activity of a dermatan sulfate from the skin of the shark Scyliorhinus canicula

Dhahri, Manel; Mansour, Mohamed Ben; Bertholon, Isabelle; Ollivier, Véronique; Boughattas, Naceur A.; Hassine, Mohsen; Jandrot‐Perrus, Martine; Chaubet, Frédéric; Maaroufi, Raoui M.

doi:10.1097/mbc.0b013e32833b643b

Cited by 13 publications

(31 citation statements)

References 41 publications

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“…It was showed that dermatan sulfate exhibited a high anticoagulant activity (Ben Mansour et al 2009a, b , 2010. Similar activity of a dermatan sulfate from the skin of the shark Scyliorhinus canicula was also reported by Dhahri et al ( 2010 ). The substance of the shark origin showed anticoagulant effect that was higher than that isolated from pork.…”

Section: Resultssupporting

confidence: 73%

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Marine Structural Proteins in Biomedicine and Tissue Engineering

Ehrlich

2014

Biological Materials of Marine Origin

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Marine biopolymers like collagens, gelatins, keratins (keratin-like proteins), and elastins (elastin-like proteins) have been investigated during the last decade as potentially approvable, manufacturable, highly reproducible, approvable, and affordable biological materials. The development of biocompatible composites and vehicles of marine biopolymer origin for growth, retention, delivery, and differentiation of stem cells is of crucial importance for regenerative medicine.All the marine structural proteins discussed have above possess high biomimetic potential. They either directly meet current challenges in many applied fi elds; or may be used as a source of bioinspiration for designing biomimetic materials and composites with hierarchical structure and appropriative mechanical properties. The application of new biomaterial technologies based on marine biopolymers offers the potential to direct the stem cell fate, targeting the delivery of cells and reducing immune rejection, thereby supporting the development of regenerative medicine (Armentano et al. 2013 ). The fi rst goal is to develop porous biodegradable composite scaffolds, which can be designed with initial properties that reproduce the tension-compression nonlinearity, viscoelasticity and anisotropy of different tissues by introducing specifi c nanostructures. The second goal is more complex, as it deals with development of artifi cial organs. However, the fi rst attempts have been carried out. Recently, dense and porous synthetic scaffolds were used as template for fi sh cells line, which, being attached, can proliferate and express fi sh skin components. This biomimetic approach has been used for development of a biosynthetic fi sh skin in vitro with future application in aquatic robots that can emulate living fi sh (Pouliot et al. 2004 ).As reported above, marine collagens of vertebrate origin as well as gelatins as their derivatives are the most popular biological materials. These can be used as alternative to terrestrial mammal's collagens especially in tissue engineering. According to Glowacki and Mizuno ( 2008 ), "there are two major approaches to tissue engineering for regeneration of tissues and organs. One involves cell-free materials and/or factors and one involves delivering cells to contribute to the regeneration process. Of the many scaffold materials being investigated, collagen type I, with selective removal of its telopeptides, has been shown to have many advantageous features for both of these approaches," (Glowacki and Mizuno 2008 ).

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Section: Resultssupporting

confidence: 73%

“…Proteoglycans (Tingbø et al 2012 ) as well as glycosaminoglycans (GAGs) like chondroitin sulfate (Hashiguchi et al 2011 ), dermatan sulfate (Chatziioannidis et al 1999 ;Sakai et al 2003 ;Dhahri et al 2010 ), aggrecan (Kakizaki et al 2011 ) and hyaluronan (Pfeiler et al 2002 ) has been also isolated from marine vertebrates, mostly from fi sh.…”

Section: Resultsmentioning

confidence: 98%

Marine Structural Proteins in Biomedicine and Tissue Engineering

Ehrlich

2014

Biological Materials of Marine Origin

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“…It is believed that these therapeutic effects are due to DS-IEL 30 binding to the vessel wall and subsequently preventing platelet binding and not through direct anticoagulant properties. Dermatan sulfate from porcine origin (which is the type used for EC-SEAL synthesis) has been shown to have very minimal anticoagulant properties depending on the concentration [54]. By measuring aPTT of whole blood that had been incubated with varying concentrations of both heparin and DS-IEL 30 , we have confirmed the minimal effect of DS-IEL 30 on clotting time (Figure 11).…”

Section: Discussionmentioning

confidence: 58%

Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction

et al. 2017

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Endothelial cell (EC) dysfunction is associated with many disease states including deep vein thrombosis (DVT), chronic kidney disease, sepsis and diabetes. Loss of the glycocalyx, a thin glycosaminoglycan (GAG)-rich layer on the EC surface, is a key feature of endothelial dysfunction and increases exposure of EC adhesion molecules such as selectins, which are involved in platelet binding to ECs. Once bound, platelets cause thrombus formation and an increased inflammatory response. We have developed a GAG derived, selectin targeting anti-adhesive coating (termed EC-SEAL) consisting of a dermatan sulfate backbone and multiple selectin-binding peptides designed to bind to inflamed endothelium and prevent platelet binding to create a more quiescent endothelial state. Multiple EC-SEAL variants were evaluated and the lead variant was found to preferentially bind to selectin-expressing ECs and smooth muscle cells (SMCs) and inhibit platelet binding and activation in a dose-dependent manner. In an in vivo model of DVT, treatment with the lead variant resulted in reduced thrombus formation. These results indicate that EC-SEAL has promise as a potential therapeutic in the treatment of endothelial dysfunction.

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“…In previous studies, we isolated and characterized a DS from the skin of the Scyliorhinus canicula shark, a nonmammalian marine product, which decreases the risk of pathogenic contamination . Furthermore, we demonstrated that soluble DS exhibited an antithrombin activity mediated by heparin cofactor II, a serine protease inhibitor . We have also shown that immobilization of DS on PET surfaces decreased fibrinogen adsorption while it increased albumin adsorption on the PET surfaces, enhanced both in vitro endothelial cell proliferation and the tissue biocompatibility in subcutaneous implantation in a rat model …”

Section: Introductionmentioning

confidence: 89%

“…DS was obtained by papain digestion followed by cetyltrimethylammonium bromide (CTAB) and ethanol precipitation then dialyzed against deionized water, and a diethylaminoethyl (DEAE)‐cellulose anion‐exchange chromatography step was performed to separate DS from hyaluronic acid. The DS fractions were then pooled and lyophilized …”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo hemocompatibility evaluation of a new dermatan sulfate-modified PET patch for vascular repair surgery

Dhahri

Rodriguez-Ruiz

Aid‐Launais

et al. 2016

J. Biomed. Mater. Res.

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The development of new vascular devices requires to study the effects of materials on blood cells and on coagulation, both in vitro and in vivo. In this study, we have developed a new material by grafting dermatan sulfate (DS) from shark skin onto polyethylene terephthalate (PET). We have evaluated the haemocompatibility of PET-DS material in vitro by measuring thrombin generation, plasma recalcification time, hemolytic activity, and platelet adhesion and in vivo with a model of vascular patch in rat abdominal aorta. In vitro, our results have shown that PET-DS is a nonhemolytic material, able to inhibit thrombin generation and platelet adhesion. In vivo studies by Doppler echographic evaluation 20 days after implantation have shown that the PET-DS patch was integrated in the vessel wall and covered by a layer of cells. In conclusion, PET-DS has good haemocompatibility properties and could be a promising tool for vascular surgery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2001-2009, 2017.

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Anticoagulant activity of a dermatan sulfate from the skin of the shark Scyliorhinus canicula

Cited by 13 publications

References 41 publications

Marine Structural Proteins in Biomedicine and Tissue Engineering

Marine Structural Proteins in Biomedicine and Tissue Engineering

Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction

In vitro and in vivo hemocompatibility evaluation of a new dermatan sulfate-modified PET patch for vascular repair surgery

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