Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

Abstract

ABSTRACT

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

“…The heparin-like anticoagulant activities of SPs from marine algae have been universally recognized [60,61]. The anticoagulant activity of SPs is likely the most important aspect of extensive explorations of marine algae in the medical field.…”

<b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds

“…According to Fonseca et al (2008) and Mourão (2015), slight differences in the proportion and/or distribution of sulfated residues in polysaccharide chains could be critical for the interaction of proteases, inhibitors and activators of the coagulation system, resulting in a distinct pattern of anti-and pro-coagulant effects and in antithrombotic action. Regarding the PT test, treatment of the human plasma with crude SPs extracts (1 mg mL -1 ) did not modify the normal values in the extrinsic coagulation pathway (data not shown - Rodrigues et al, 2011). …”

“…(p > 0.05), but 9-to 12-fold lower than that of UHEP, which had a marked increment on intrinsic system already at low concentrations. Using these same macroalgae species, Rodrigues et al (2011;2012b) recorded effects of 24.62 and 72.66 IU mg -1 from major polysaccharidic fractions, respectively. The tested samples of A. muscoides and C. racemosa showed modest inhibitory effects corresponding to 2.81 and 7.88 IU mg -1 , respectively, as described by Quinderé et al (2014) and Rodrigues et al (2016b;2012c), who previously reported reduced anticoagulation induced by their SPs extracted with papain.…”

“…After that, 100 μL PT reagent from Wiener Lab (Rosario, Argentina) was added to the mixtures, and the clotting time was recorded using the same coagulation equipment. The polysaccharide concentrations and the curve of unfractionated heparin (UHEP) (with 193 international units per mg ) from Europharma Lab, São Paulo, Brazil, on both tests were determined as described by Rodrigues et al (2011). Tests were performed in triplicate.…”

“…J. Agardh; and of Chlorophyceae Caulerpa cupressoides var. lycopodium C. Agardh and C. racemosa (Forsskal) J. Agardh are commonly found along the Northeastern coast of Brazil and studies demonstrated SPs with pharmacological value as anticoagulant (Fidelis et al, 2014;Quinderé et al, 2014;Rodrigues et al, 2011;2012b;Rodrigues, Quinderé, Queiroz, Coura, & Benevides, 2012c;Rodrigues et al, 2013;2016b), analgesic/anti-inflammatory Rodrigues et al, 2012d;Ribeiro et al, 2014) and antiviral (Vanderlei et al, 2016;Rodrigues et al, 2017) for biotechnology. No examination on the in vitro anticoagulant potentials of crude SPs from these species using TG assays has been revealed so far.…”