Anticoagulant, antiplatelet and fibrin clot hydrolyzing activities of flax seed buffer extract

Nandish, Sharath Kumar M.; Kengaiah, Jayanna; Ramachandraiah, Chethana; Shivaiah, Ashwini; Chandramma,; Girish, K. S.; Kemparaju, K.; Sannaningaiah, Devaraja

doi:10.4103/pm.pm_320_17

Cited by 14 publications

(2 citation statements)

References 3 publications

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“…Plasma clotting times in PT were solely lowered by NCs concentration, pointing to an extrinsic route. NCs, which were used to observe the procoagulant action, showed that the extrinsic route was speci cally interfered [30]. Because of this, it may be a better option for the care of pathophysiological diseases such as hemophilia and severe hemorrhage.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Biogenic approach for synthesis of ZnO/NiO nanocomposites as a highly efficient photocatalyst and evaluation of their biological properties

Puttaraju,

Soundarya,

Nagaraju

et al. 2023

Braz. J. Chem. Eng.

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Mixed metal oxide-based nanocomposites (NCs) had remained broadly used for photo catalysisfacilitated elimination of harmful substances from the aquatic ecosystem. Now a days searching for an improved photo catalyst looks plentiful metal oxide-based materials take began out of studies. In present works concerning the use of ZnO/NiO NCs as photocatalytic treatment of wastewater utilizing oxides of metals as photo catalysts has merged a subject of major concern. The way of coupling with other semiconductors for improved photo degradation in the presence of UV-visible light. NCs were mainly characterized by XRD, FT-IR, UV-DRS, morphological studies such as SEM and TEM, elemental composition by EDAX that all a rmed the effective synthesis of NCs. For instance, ZnO/NiONCs having a bandgap of about 3.11eV achieved an outstanding degradation activity toward 100% photo degradation of methylene blue (MB) within a short period of time. Further, prepared ZnO/NiO NCs have excellent antibacterial activity against both Gram negative and Gram-positive bacteria. This behavior is primarily caused by the accumulation of ZnO/NiO NCs on the bacteria's surface, which results in cytotoxic bacteria and a relatively increased ZnO, resulting in cell death. Also ZnO/NiO NCs exhibit essential harmless effects on human red blood cells and their intervention with the action of the clotting on both PPP and PRP in human erythrocytes. As an outcome, the studies have shown that Phyllanthus Niruri (L) mediated Green Synthesis by combustion methods performs well as a good capping agent to synthesize ZnO/NiO NCs with important multi-disciplinary applications.Extending laboratory and characterization facilities. Authors also thank DST-

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Section: Antibacterial Activitymentioning

confidence: 99%

Biogenic approach for synthesis of ZnO/NiO nanocomposites as a highly efficient photocatalyst and evaluation of their biological properties

Puttaraju,

Soundarya,

Nagaraju

et al. 2023

Braz. J. Chem. Eng.

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“…Several physiological agonists namely ADP, epinephrine, thrombin, thromboxane, arachidonic acid and collagen, activates platelets (Kim et al 2015). These activated platelets plug at the site of injury along with fibrin clot and inhibit blood loss (Sannaningaiah et al 2018). While, in case of hyper coagulation disorders, there will be burst of activation of platelets too contribute for the formation unusual clot (Majumdar et al 2015).…”

Section: Anticoagulant Activity and Antiplatelet Activitymentioning

confidence: 99%

Anticoagulant and antiplatelet activities of novel serine protease purified from seeds of Cucumis maderaspatensis

et al. 2021

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In this study, we report the biochemical characterization of a novel serine protease from seeds of Cucumis maderaspatensis, aimed with assessing the anticoagulant and antiplatelet activities. The purified serine protease was obtained by subjecting the seed extract to ammonium sulphate precipitation followed by anion exchange and gel filtration chromatography. Twenty seven-fold purification with the specific activity of 884.2 U/mg of protease activity was obtained. The characterization of the novel protease enzyme activity for optimum temperature, pH and effect of different protease inhibitors and metal ions were measured using caseinolytic assay and casein zymogram. The relative molecular mass of the novel neutral serine protease (CmSP) is ~ 32 kDa. Its anticoagulant was determined by assessing the delay in plasma re-calcification time in both platelet-rich and platelet-poor plasma. The antiplatelet activity of serine protease was demonstrated by inhibition of agonists induced platelet aggregation; it was in the order of Epinephrine > Adenosine tri phosphate. Further studies would decipher the mechanism of action to understand its therapeutic potential as an antiplatelet and anticoagulant molecule.

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Synthesis, structural characterization, and evaluation of new peptidomimetic Schiff bases as potential antithrombotic agents

et al. 2022

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New Schiff bases functionalized with amide and phenolic groups synthesized by the condensation of 2-hydroxybenzaldehyde and 2-hydroxyacetophenone with amino acid amides which in turn were prepared in two steps from N -Boc-amino acids and homoveraltrylamine through intermediate compounds N -Boc-amino acids amides. The compounds were characterized by elemental analysis, FT-IR, UV–Vis, and NMR spectroscopy. The crystal structures of three Schiff bases were determined by single crystal X-ray diffraction. There exists O–H N, N–H O, and C–H O types of hydrogen bonds and C–H secondary bonding interactions in these crystalline solids. The Schiff bases have been screened for anticoagulant and antiplatelet aggregation activities. All the compounds showed procoagulant activity which shortens the clotting time of citrated human plasma in both platelet-rich plasma and platelet-poor plasma except the derivatives of L-methionine which showed anticoagulant activity by prolonging the clotting time. In addition, the compounds derived from benzyl cysteine and phenylalanine showed adenosine diphosphate induced antiplatelet aggregation activity, whereas others did not show any role. Moreover, all these compounds revealed non-hemolytic activity with red blood cells. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00706-022-02936-6.

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Anticoagulant, antiplatelet and fibrin clot hydrolyzing activities of flax seed buffer extract

Cited by 14 publications

References 3 publications

Biogenic approach for synthesis of ZnO/NiO nanocomposites as a highly efficient photocatalyst and evaluation of their biological properties

Biogenic approach for synthesis of ZnO/NiO nanocomposites as a highly efficient photocatalyst and evaluation of their biological properties

Anticoagulant and antiplatelet activities of novel serine protease purified from seeds of Cucumis maderaspatensis

Synthesis, structural characterization, and evaluation of new peptidomimetic Schiff bases as potential antithrombotic agents

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