Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques

Amer, Asmaa A.; Elgohary, Rania; Ibrahim, Faten M.; Taha, Hussein

doi:10.1016/j.heliyon.2023.e15444

Cited by 3 publications

(8 citation statements)

References 39 publications

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“…In this study, we also reported an increased number of metabolites in cell cultures versus floral buds as compared to the previous work reported by Amer et al. (2023) , in which they reported fewer total phenolic and flavonoids in the cell cultures from bioreactors when compared to the leaves.…”

Section: Discussionsupporting

confidence: 62%

“…In other species, such as the Brazilian Hancornia speciosa (Gomes) cell suspension cultures, adding 3% glucose increased fresh weight and production of polyphenols ( Dantas et al., 2021 ). The differences seen in the period for achieving the maximum growth during the growth curve between Amer et al. (2023) and the current study, 14 and 30 days, respectively, could be attributed to the differences in the varieties of feijoa, the different source tissue used for induction of callus as well as the differences in the set of growth conditions.…”

Section: Discussionmentioning

confidence: 56%

“…Feijoa extracts have been tested for their efficacy against various human ailments. More recently, bioactive compounds were identified and assessed from feijoa callus and cell suspensions for their anticoagulant properties, which resulted in decreased platelet aggregation ( Amer et al., 2023 ). The source of these extracts has so far been the fruit or the fruit peel.…”

Section: Discussionmentioning

confidence: 99%

“…Different plant groups respond differently to the callus induction process, which depends on many factors, such as types and concentrations of auxins and cytokinins, the tissue and the physiological state of the tissue. During earlier research studies on feijoa, where application of callus has been majorly restricted to achieve somatic embryogenesis, callus had been induced from stamen filaments, petals and ovaries ( Stefanello et al., 2005 ), whole intact cotyledonary zygotic embryos ( Reis et al., 2008 ), fruit pulp ( Verardo et al., 2019 ) and leaves ( Amer et al., 2023 ). In this study, we used floral buds without separating stamen filaments, petals, or ovaries but used the regions of floral buds as explants ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…(2023) and the current study, 14 and 30 days, respectively, could be attributed to the differences in the varieties of feijoa, the different source tissue used for induction of callus as well as the differences in the set of growth conditions. Even though applications of feijoa cell suspensions for bioreactor setup have been mentioned recently ( Amer et al., 2023 ) investigating the anticoagulant properties of feijoa, there has been a lack of data on the effect of different parameters involved in generating cell suspensions from feijoa. The current study has investigated and presented for the first time the effect of different parameters involved in establishing feijoa cell suspension cultures aimed at generating biomass for the extraction of bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of feijoa (Acca sellowiana) callus and cell suspension cultures and identification of arctigenin - a high value bioactive compound

Raikar,

Isak,

Patel

et al. 2024

Front. Plant Sci.

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Feijoa (Acca sellowiana (O. Berg.) Burret), also known as pineapple guava, is a member of the Myrtaceae family and is well known for its fruit. Chemical profiling of the different tissues of the feijoa plant has shown that they generate an array of useful bioactive compounds which have health benefits such as significant antioxidant activities. In this study, an in vitro culture system has been developed, which could be explored to extract high-value bioactive compounds from feijoa. Feijoa tissue culture was initiated by the induction of callus from floral buds. Sections of floral buds were plated on MS medium supplemented with 2,4-D and BAP at 2.0mg/L and 0.2mg/L concentrations, respectively. Cell suspension cultures of feijoa were established using a liquid MS medium with different concentrations of 2,4-D and BAP and cultured on a rotary shaker. The growth of the cell suspension was evaluated with different parameters such as different carbohydrate sources, concentration of MS media, and inoculum density. When the cell suspensions were treated with different concentrations of MeJA at different time points, phytochemicals UPLC - QTOF MS analysis identified extractables of interest. The main compounds identified were secondary metabolites (flavonoids and flavonoid-glucosides) and plant hormones. These compounds are of interest for their potential use in therapeutics or skin and personal care products. This report investigates essential methodology parameters for establishing cell suspension cultures from feijoa floral buds, which could be used to generate in vitro biomass to produce high-value bioactive compounds. This is the first study reporting the identification of arctigenin from feijoa, a high-value compound whose pharmaceutical properties, including anti-tumour, anti-inflammatory and anti-colitis effects, have been widely reported. The ability of feijoa cell cultures to produce such high-value bioactive compounds is extremely promising for its use in pharmaceuticals, cosmeceuticals and nutraceuticals applications.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Establishment of feijoa (Acca sellowiana) callus and cell suspension cultures and identification of arctigenin - a high value bioactive compound

Raikar,

Isak,

Patel

et al. 2024

Front. Plant Sci.

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Biochemical and biological studies of irradiated and non-irradiated extracts of Solanum aculeastrum Dunal fruit

Amer,

Soliman,

Alshareef

et al. 2024

Sci Rep

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This study explores the impact of γ-irradiation on ethanolic extracts of Solanum aculeastrum Dunal. The anti-cancer and antimicrobial properties were investigated. The obtained results revealed that total phenol (TP) and total flavonoid (TF) of total ethanol extract (100%) (FTE) were higher than 70% ethanol extract (SE), and these contents increased after gamma radiation with 5 kGy. The results of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the Solanum aculeastrum extracts suggested that FTE and 5 kGy-irradiated FTE can be used to control and prevent skin infections caused by MRSA and endocarditis, urinary tract infections, and prostatitis caused by Enterococcus faecalis. The FTE sample irradiated at 5 kGy showed cytotoxicity for A431 and Hct-116 cell lines similar to the control sample and higher than the toxicity revealed by the samples irradiated at 10 kGy. In normal cells (Bj-1), the toxicity was decreased after irradiation (IC50 = 31 μg/ml) compared to the non-irradiated extract (IC50 = 26.1 μg/ml). Molecular docking suggested Sortase A to play a role in chlorogenic acid antibacterial activity towards Staphylococcus aureus. In conclusion, γ-irradiation can be used to enhance the phytoconstituents of Solanum aculeastrum fruit extracts and, consequently, its biological properties.

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The Role of Anticoagulant, Thrombolytic, and Fibrinolytic Activities in the Prevention of Peritoneal Adhesion

DEYNEZ,

MİSER SALİHOĞLU,

SÜNTAR

2023

Trakya University Journal of Natural Sciences

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Peritoneal adhesion occurs as a result of surgery, peritoneal injury, peritonitis, hypoxia, and ischemia. Surgical trauma causes many pathophysiological processes which include inflammation, oxidation, coagulation, fibrinolysis, cell proliferation, and apoptosis. After intra-abdominal operations, the adhesion tissue may occur on the peritoneal surface due to low fibrinolytic activity. This may result in permanent excessive adhesion tissue bands instead of properly formed fibrin structures. Therefore, anticoagulant, thrombolytic, and fibrinolytic activities have a key role in preventing peritoneal adhesion. Indeed, several studies have been conducted to find out new and effective agents against intra-abdominal adhesion. Thus, revealing the causes, development processes, and investigation techniques are highly important for designing and conducting such scientific studies. In this context, this study aims to summarize the pathophysiological processes of above-mentioned activities and to emphasize their importance in the peritoneal adhesion model as well as to explain the evaluation methods, particularly in terms of the investigation of natural products.

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Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques

Cited by 3 publications

References 39 publications

Establishment of feijoa (Acca sellowiana) callus and cell suspension cultures and identification of arctigenin - a high value bioactive compound

Establishment of feijoa (Acca sellowiana) callus and cell suspension cultures and identification of arctigenin - a high value bioactive compound

Biochemical and biological studies of irradiated and non-irradiated extracts of Solanum aculeastrum Dunal fruit

The Role of Anticoagulant, Thrombolytic, and Fibrinolytic Activities in the Prevention of Peritoneal Adhesion

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