Anticoagulant Properties of a New Anticoagulant Diethyl Citrate in Rabbit Model

Abstract

A novel anticoagulant, diethyl citrate (Et 2 Cit) was synthesized and characterized by carbon nuclear magnetic resonance (13 C NMR), mass spectrometry and UV-visible spectroscopy. There were two isomers with different chemical environments, that is, 1, 3-diethyl citrate and 1,5-diethyl citrate. Through the determination of whole blood activated clotting time (ACT), the anticoagulant effect in vitro of Et 2 Cit was investigated. Et 2 Cit can reduce ions calcium concentration in blood then to play the role of anticoagulation. Et 2 Cit was superior to anticoagulant Na 3 Cit duing to the steric hindrance of two ethyl groups in citric acid, which leading a decrease of chelated ability compared to that of Na 3 Cit. It can prevent hypocalcemia when Et 2 Cit as anticoagulation, due to the ion blood calcium concentration resumed faster than Na 3 Cit. Consequently Et 2 Cit may be a potential ideal anticoagulant.