Anticoagulant Property of a Sulfated Polysaccharide with Unique Structural Characteristics from the Green Alga Chaetomorpha aerea

Abstract

Sulfated polysaccharides from marine algae have attracted a great amount of attentions for the development of marine drugs due to their unique structural features, and they are great potential sources of naturally occurring anticoagulant agents. The genus Chaetomorpha is one of the largest genera in green algae and has a worldwide distribution. In the present study, a homogeneous polysaccharide from Chaetomorpha aerea, designated as PCA, was obtained by alkali extraction, anion-exchange and size-exclusion chromatography. Based on the results of chemical and spectroscopic analyses, PCA was a sulfated galactoarabinan which was mainly constituted of a backbone of →4)-β-l-Arap-(1→ unit, partially sulfated at C-3 of →4)-β-l-Arap-(1→ and C-4 of →6)-α-d-Galp-(1→. The side chains consisting of →6)-α-d-Galp-(1→ and →5)-α-l-Araf-(1→ residues were in C-2 of →4)-β-l-Arap-(1→ unit. PCA had a strong anticoagulant activity in vitro as evaluated by the assays of activated partial thromboplastin time, thrombin time and fibrinogen level. The obvious anticoagulant activity in vivo of PCA was also found. PCA significantly inhibited the activities of the intrinsic coagulation factors XII, XI, IX and VIII, and exhibited weak inhibition effects on the common coagulation factors II and X. The anticoagulant mechanism of PCA was attributed to strong thrombin inhibition potentiated by heparin cofactor II or antithrombin III, and it also possessed an apparent inhibition effect on coagulation factor Xa mediated by antithrombin III. The investigation demonstrated that PCA could be a promising anticoagulant agent for health promotion and the treatment of thrombotic diseases.

Exploring Bioactive Components and Assessing Antioxidant and Antibacterial Activities in Five Seaweed Extracts from the Northeastern Coast of Algeria

“…In our current study, C. laetevirens had an ulvan extraction yield of 2.78 ± 0.07%, slightly lower than the ulvan extraction yield of C. aerea collected in Yantai, China, at 5.65% [ 40 ].…”

Chemical diversity and therapeutic potentialities of seaweeds and marine sponges collected from the Red Sea: An update

“…In the same context, a diverse array of chemical compounds has been isolated or identified from different marine organisms such as alkaloids (Moriou et al, 2021;Tempone et al, 2021), anthraquinones (Chen et al, 2022), peptides (Ghareeb et al, 2020), polysaccharides (Ghareeb et al, 2020), polyketides (Ghareeb et al, 2020), and terpenes (Chen et al, 2022). Various extracts of marine organisms and/or their pure isolates exhibited a broad spectrum of bioactivities like antimicrobial (Krome et al, 2022;Liang et al, 2023), antioxidant (Catarino et al, 2023Hamed et al, 2020), anti-Gyr-B enzyme (Agour et al, 2022), antiallergic (Xie et al, 2017), antibiofilm (Cepas et al, 2019), anticancer (Agena et al, 2023;Panggabean et al, 2022), anti-inflammatory (Ghareeb et al, 2020;Rocha et al, 2022), anticoagulant (Qin et al, 2023), antiparasitic (Mostafa et al, 2022), antiallergic (Chen et al, 2023), and antiaging (Yang et al, 2023a(Yang et al, , 2023b. The marine environment is considered a strategic treasure and a huge warehouse for the production of bioactive compounds, which act as lead compounds in the pharmaceutical industry.…”