Anticoagulant surface modification of titanium via layer‐by‐layer assembly of collagen and sulfated chitosan multilayers

Li, Quanli; Huang, Nan; Chen, Jialong; Wan, Guojiang; Zhao, Ansa; Chen, Junying; Wang, Jin; Yang, Ping; Leng, Y.X.

doi:10.1002/jbm.a.31999

Cited by 48 publications

(25 citation statements)

References 40 publications

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“…Further, the co-existence of heparin with antithrombin (covalent bonding)43 or chitosan (blending)44 result improved anti-coagulatory performance of biomaterials. Other attempts include the coating of methacryloylphosphorycholine laurylmethacrylate copolymer45, multi-layer of collagen and sulphated chitosan46, surface immobilization of phosphatidyl choline47 and ionical deposition of thermoplastic polyurethane on material surface48. The sulfonation of silk fibroin also improves its anticoagulant activity49.…”

Section: Discussionmentioning

confidence: 99%

Thromboelastometric and platelet responses to silk biomaterials

Kundu

Schlimp

Nürnberger

et al. 2014

Sci Rep

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Silkworm's silk is natural biopolymer with unique properties including mechanical robustness, all aqueous base processing and ease in fabrication into different multifunctional templates. Additionally, the nonmulberry silks have cell adhesion promoting tri-peptide (RGD) sequences, which make it an immensely potential platform for regenerative medicine. The compatibility of nonmulberry silk with human blood is still elusive; thereby, restricts its further application as implants. The present study, therefore, evaluate the haematocompatibility of silk biomaterials in terms of platelet interaction after exposure to nonmulberry silk of Antheraea mylitta using thromboelastometry (ROTEM). The mulberry silk of Bombyx mori and clinically used Uni-Graft W biomaterial serve as references. Shortened clotting time, clot formation times as well as enhanced clot strength indicate the platelet mediated activation of blood coagulation cascade by tested biomaterials; which is comparable to controls.

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Section: Discussionmentioning

confidence: 99%

Thromboelastometric and platelet responses to silk biomaterials

Kundu

Schlimp

Nürnberger

et al. 2014

Sci Rep

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“…The deposition cycle creates a bilayer, and these cycles can be repeated as often as needed. The LBL technique allows the layer thickness and interlayer separation to be controlled on the nanometer scale, with controlled surface structure and charge, which in turn govern biological responses 32–34. The technique is not restricted by substrate shape and can be used for coating arbitrarily shaped objects.…”

Section: Introductionmentioning

confidence: 99%

Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Chen

et al. 2010

J Biomedical Materials Res

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Thrombosis and restenosis are the main causes leading to failure of cardiovascular and other blood-contacting biomedical devices. It is recognized that rapid re-endothelialization is a promising method for preventing these complications. This article deals with improving the endothelial progenitor cell (EPC) compatibility and hemocompatibility of titanium by coating an extracellular matrix-like film with heparin(hep) and collagen(col) by a layer-by-layer (LBL) self-assembly technique. In the work described here, LBL-produced col/hep coating growth is initialized by deposition of a layer of poly-L-lysine on a titanium surface, which is negatively charged after treatment with NaOH, followed by formation of a multilayer film formed by alternating deposition of negatively charged heparin and positively charged collagen using electrostatic interaction. The X-ray photoelectron spectroscopy results and fluorescence staining of collagen show that collagen is predominant on the surface and that collagen interpenetrates the heparin layer. In vitro EPC attachment and proliferation increase greatly on the col/hep coating. Immunofluorescent staining of cytoskeleton actin reveals that cells on the col/hep coating form a compact confluent cell layer after culture for 3 days. After culture for 5 days, cell viability on the col/hep increases persistently and on titanium the cell viability begins to decrease, showing that the coating possesses the ability to maintain cell viability. Platelet adhesion under dynamic conditions in vitro implies that the hemocompatibility of the col/hep coating is superior to that of titanium. The col/hep coating improves the biocompatibility of titanium and has good potential for application in blood-contacting biomaterials.

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“…It is expected that the collagen component of the multilayer will promote endothelial cell adhesion and growth on the surface [218]. The electrostatic attraction between NH 3 + groups of collagen and SO 3 − groups of sulfated chitosan is the main driving force leading to LBL assembly.…”

Section: Chitosan/protein Self-assemblymentioning

confidence: 99%

Chitosan Derivatives

2016

Chitosan-Based Hydrogels

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Anticoagulant surface modification of titanium via layer‐by‐layer assembly of collagen and sulfated chitosan multilayers

Cited by 48 publications

References 40 publications

Thromboelastometric and platelet responses to silk biomaterials

Thromboelastometric and platelet responses to silk biomaterials

Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Chitosan Derivatives

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