Anticoagulants and Other Preanalytical Factors Interfere in Plasma Nitrate/Nitrite Quantification by the Griess Method

Ricart-Jané, David; Llobera, Miquel; López‐Tejero, M. Dolores

doi:10.1006/niox.2001.0392

Cited by 117 publications

(70 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Varicocele is related with both increased generation of nitric oxide (NO) and spermatozoal reactive species. NO that is synthesized by the enzyme NOS, with low concentration has its important biological roles in male reproductive function, but excessive production of NO have negative effects on sperm parameters quality (Romeo et al;Ricart-Jané et al;Ramya et al, 2011). Our study has showed a significant increase of NOx in the serum of varicocele groups.…”

Section: Discussionmentioning

confidence: 48%

“…The second step is the addition of Griess reagent, which converts nitrite in to the deep purple azo compound; photometric measurement of the absorbance at 540 nm due to this azo chromophore accurately determines the nitrite concentration (sodium nitrate is used as a standard). Protein interference is removed by treating the reacted samples with zinc sulphate and centrifuging them for 10 min at 2000 g (Schulz et al, 1999;Aksoy et al;Ricart-Jané et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Effects of Curcumin on Sperm Parameters and Nitric Oxide Production in Varicocelized Rats

et al. 2015

View full text Add to dashboard Cite

SUMMARY:One of the proposed mechanism by which varicocele induces its damage is excessive release of nitric oxide (NO). Several studies have shown the role of NO in poor sperm quality in infertile patients with varicocele. Scientific studies have demonstrated the beneficial effects of curcumin on the sperm parameters. Curcumin as an atoxic antioxidant can reduce production of NO. The aim of this study was to determine the effect of curcumin on NO levels and investigate if curcumin can improve sperm parameters in varicocelized male rats. Thirty male Wistar rats were randomly divided into 5 groups (V1 and V2 (varicocele), T (treatment), Sh (sham) and C was control). In groups V1, V2, T and Sh, the left renal vein was partially ligated to induce varicocele. In groups V1 and V2, sperm parameters and NO level were evaluated 8 and 16 weeks respectively after varicocele induction. Groups T and Sh received 100 mg/kg curcumin and placebo respectively, daily for 8 weeks after 2 months of induced varicocele. Sperm parameters (count, motility, viability and morphology), epididymis and testis weight and also NO concentration were measured. Sperm parameters (count, motility and viability) in groups V1, V2 and Sh were significantly low in comparison with control and treatment groups. The level of NO was significantly increased in serum of rats in groups V1 and V2, whereas group T rat serum in which curcumin was administered, showed decreased NO levels. The values of the epididymis and testis weight had no significant changes (P≤ 0.05) in all groups. Administration of curcumin as a free radical scavenger, can decrease NO level and improve sperm parameters in varicocelized male rats.

show abstract

Section: Discussionmentioning

confidence: 48%

Section: Methodsmentioning

confidence: 99%

The Effects of Curcumin on Sperm Parameters and Nitric Oxide Production in Varicocelized Rats

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Nitrate determination requires chemical and enzymatic reduction to nitrite. This method is easy, quick, inexpensive, and more sensitive (to micromolar range) than other methods [39].…”

Section: Quantification Of Nitrate/nitrite Content In Gcfmentioning

confidence: 93%

Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

Güzeldemir

Toygar²,

Bal³

et al. 2011

Turk J Hematol

View full text Add to dashboard Cite

Objective: This study aimed to biochemically measure the production of nitric oxide in gingival crevicular fluid and immunohistochemically measure the expression of inducible nitric oxide synthase in the gingiva of patients with sickle cell disease. Additionally, we aimed to obtain insight into the immunopathology of sickle cell disease by comparing inducible nitric oxide synthase levels in patients with sickle cell disease and controls using gingiva and gingival crevicular fluid. Materials and Methods: The study included 20 sickle cell disease patients and 20 healthy controls. Immunohistochemical analysis was used to measure inducible nitric oxide synthase expression in gingiva and nitric oxide levels in gingival crevicular fluid were spectrophotometrically measured. Results: Nitric oxide levels in the patients and controls did not differ significantly (21.2±4.5 and 23.1±2.3 μM L -1 , respectively, p>0.05). There weren't any statistically significant differences in infiltrated inflammatory cells, density of inflammatory cells that stained with inducible nitric oxide synthase, or nitric oxide expression in gingiva between the patient and control groups (p>0.05). Conclusion: To the best of our knowledge this is the first study to examine the expression of inducible nitric oxide synthase in the gingiva and gingival crevicular fluid in patients with sickle cell disease. Using the gingiva and gingival crevicular fluid we were unable to observe sickle cell disease-associated inducible nitric oxide synthase expression and a difference in nitric oxide levels.

show abstract

“…Alguns estudos sugerem que o EDTA é o melhor anticoagulante em comparação com a heparina e o citrato para a quantificação dos metabólitos do óxido nítrico, sendo o mais usado em rotinas clínicas e experimentais (9) . No entanto, neste estudo conduzido por Ricart-Jané et al, foram determinados simultaneamente os níveis de nitrato e nitrito (NOx) em amostras de plasma, não sendo avaliados os níveis de NOx em amostras de soro (9) . Outros estudos demonstraram que o plasma heparinizado pode formar um precipitado sobre a adição do reagente de Griess, tornando este anticoagulante desfavorável para a análise (3) .…”

Section: Figura -Níveis De Nitrito Obtidos Nas Amostras De Soro E Plaunclassified

“…A determinação indireta do NO pode ser realizada por dosagem plasmá-tica ou urinária dos seus metabólitos nitrato e nitrito, sendo que o método frequentemente utilizado na mensuração desses íons baseia-se na reação de Griess (2) . Os valores de nitrito obtidos pela reação de Griess podem sofrer influências de acordo com o tipo de anticoagulante utilizado na coleta (9) . Desta forma, este trabalho visa avaliar a influência de algumas variáveis pré-analíticas como o tipo de anticoagulante utilizado na colheita e a temperatura de armazenamento da amostra sobre os níveis sanguíneos de nitrito mensurados pelo método de Griess automatizado.…”

Section: Introductionunclassified

Influência dos anticoagulantes e da temperatura de armazenamento sobre os níveis sanguíneos de nitrito

Tatsch¹,

Bochi

Pereira³

et al. 2011

J. Bras. Patol. Med. Lab.

View full text Add to dashboard Cite

A mensuração dos metabólitos do óxido nítrico pode ser útil para a melhor compreensão de diferentes processos fisiopatológicos. Este estudo avaliou a influência de diferentes anticoagulantes (ácido etilenodiaminotetracético [EDTA], citrato e heparina) e do armazenamento a -20ºC por quatro meses sobre os níveis de nitrito, sendo estes mensurados em amostras de soro e plasma pelo método de Griess. Os tipos de amostra utilizados (soro ou plasma) e de anticoagulante usado na coleta não influenciaram significativamente os níveis de nitrito, independentemente de as dosagens serem realizadas em amostras frescas ou naquelas mantidas a -20ºC por quatro meses.

show abstract

Anticoagulants and Other Preanalytical Factors Interfere in Plasma Nitrate/Nitrite Quantification by the Griess Method

Cited by 117 publications

References 17 publications

The Effects of Curcumin on Sperm Parameters and Nitric Oxide Production in Varicocelized Rats

The Effects of Curcumin on Sperm Parameters and Nitric Oxide Production in Varicocelized Rats

Nitric oxide in gingival crevicular fluid and nitric oxide synthase expression in the gingiva of patients with sickle cell disease

Influência dos anticoagulantes e da temperatura de armazenamento sobre os níveis sanguíneos de nitrito

Contact Info

Product

Resources

About