Anticoccidial and Antioxidant Activities of Moringa oleifera Leaf Extract on Murine Intestinal Eimeriosis

Abdel-Tawab, Heba; Abdel-Haleem, Heba M.; Abdel‐Baki, Abdel‐Azeem S.; Al‐Quraishy, Saleh; El-Mallah, Al-Mahy

doi:10.2478/s11686-020-00219-w

Cited by 10 publications

(19 citation statements)

References 35 publications

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“…( 59 ), and Abdel-Tawab et al. ( 30 ) reported that macrophages produce NO by oxidizing the guanidino nitrogen of L-arginine by an enzyme, nitric oxide synthase (NOS). This enzyme is inducible in macrophages by pathogen endotoxins and is termed inducible NOS (iNOS).…”

Section: Discussionmentioning

confidence: 99%

“…Mice were divided into five groups of five mice per group, as follows: G-I, Non-infected, non-treated (negative control), G-II, Non-infected, treated group with 200 mg/kg KLRE ( 28 ), G-III, Infected, non-treated (positive control), G-IV, Infected and treated group with 200 mg/kg KLRE ( 28 ), and G-V: Infected and treated group with 120 mg/kg Amprolium ( 29 ) for 5 days. All groups (except 1 and 2) were orally infected with 10 3 sporulated E. papillata oocysts in 100 µl physiological saline ( 30 ).…”

Section: Methodsmentioning

confidence: 99%

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Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Abdel-Gaber,

Alamari,

Dkhil

et al. 2024

Front. Immunol.

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IntroductionRecently, the use of botanicals as an alternative to coccidiostats has been an appealing approach for controlling coccidiosis. Therefore, this study was conducted to evaluate the potential role of aqueous methanolic extract (200 mg/kg) of Krameria lappacea (roots) (KLRE) against infection induced by Eimeria papillata.MethodsA total of 25 male C57BL/6 mice were divided into five groups (I, II, III, IV, and V). On 1st day of the experiment, all groups except groups I (control) and II (non-infected-treated group with KLRE), were inoculated orally with 103 sporulated E. papillata oocysts. On the day of infection, group IV was treated with KLRE. Group V served as an infected-treated group and was treated with amprolium (coccidiostat).ResultsTreatment with extract and coccidiostat was continued for five consecutive days. While not reaching the efficacy level of the reference drug (amprolium), KLRE exhibited notable anticoccidial activity as assessed by key criteria, including oocyst suppression rate, total parasitic stages, and maintenance of nutrient homeostasis. The presence of phenolic and flavonoid compounds in KLRE is thought to be responsible for its positive effects. The Eimeria infection increased the oxidative damage in the jejunum. KLRE treatment significantly increased the activity of catalase and superoxide dismutase. On the contrary, KLRE decreased the level of malondialdehyde and nitric oxide. Moreover, KLRE treatment decreased macrophage infiltration in the mice jejunal tissue, as well as the extent of CD4 T cells and NFkB. E. papillata caused a state of systemic inflammatory response as revealed by the upregulation of inducible nitric oxide synthase (iNOs)-mRNA. Upon treatment with KLRE, the activity of iNOs was reduced from 3.63 to 1.46 fold. Moreover, KLRE was able to downregulate the expression of pro-inflammatory cytokines interferon-γ, nuclear factor kappa B, and interleukin-10 -mRNA by 1.63, 1.64, and 1.38 fold, respectively. Moreover, KLRE showed a significant reduction in the expression of IL-10 protein level from 104.27 ± 8.41 pg/ml to 62.18 ± 3.63 pg/ml.ConclusionCollectively, K. lappacea is a promising herbal medicine that could ameliorate the oxidative stress and inflammation of jejunum, induced by E. papillata infection in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Abdel-Gaber,

Alamari,

Dkhil

et al. 2024

Front. Immunol.

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“…Unfortunately, neither physical nor chemical medications have much effect on the Eimeria oocyst wall (Mai et al, 2009). Recently, Numerous in vitro studies have confirmed that herbal extracts are effective against coccidiosis in birds (Abdel- Tawab et al, 2020;Balta et al, 2021;Yang et al, 2019;Yong et al, 2020). Acacia is native to Egypt and widely distributed worldwide in tropical and subtropical countries like Asia, Australia, Africa, and America (Spicer et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

An in vitro evaluation of the inhibitory effects of an aqueous extract of Acacia nilotica on Eimeria tenella.

Esraa¹,

Abdelmageed

Omar

et al. 2022

SVU-International Journal of Veterinary Sciences

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Eimeria tenella is one of the most important species of Eimeria that infect domestic fowl, causing coccidiosis in the poultry industry associated with drastic economic loss. Alternative treatment options are often necessary since anticoccidial drugs are prohibitively expensive, have serious side effects, or develop resistance. The role that herbal therapy plays in basic healthcare has been rediscovered worldwide. Consequently, our research assessed the in vitro inhibitory effect of escalated concentrations (6.25 mg, 12.5 mg, 25 mg, 50 mg, and 100 mg/ml) of Acacia nilotica aqueous extract (ANAE) on Eimeria tenella sporulation. Statistical analysis revealed that ANAE decreased the percentage of oocyst sporulation in a dose-dependent manner. Furthermore, ANAE showed abnormal sporulation and morphological deterioration of E. tenella oocytes. Area Under the Curve (AUC) calculation was used to determine the efficacy of ANAE and revealed that ANAE concentrations significantly reduced the coccidial score index. At 100 mg/ml, ANAE completely suppressed the sporulation of E. tenella oocysts, with obvious changes to their morphology and size. The phytochemical analysis of ANAE has shown that ANAE contains several active principles that possess anthelmintic activities. These compounds include tannins, saponins, flavonoids, terpenoids, and alkaloids, which can be attributed to the anticoccidial activity of ANAE. Considering our findings, we recommend that ANAE be used to prevent and control Eimeria.

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“…This instigates, that utilizing medicinal plants in poultry farming can result in healthier animals, increased production, and decreased reliance on antibiotics. 175 …”

Section: Preventive Measures Of Spread Of Antimicrobial Resistance Fr...mentioning

confidence: 99%

Potential Causes of Spread of Antimicrobial Resistance and Preventive Measures in One Health Perspective-A Review

Endale,

Mathewos,

Abdeta

2023

IDR

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Antimicrobial resistance, referring to microorganisms’ capability to subsist and proliferate even when there are antimicrobials is a foremost threat to public health globally. The appearance of antimicrobial resistance can be ascribed to anthropological, animal, and environmental factors. Human-related causes include antimicrobial overuse and misuse in medicine, antibiotic-containing cosmetics and biocides utilization, and inadequate sanitation and hygiene in public settings. Prophylactic and therapeutic antimicrobial misuse and overuse, using antimicrobials as feed additives, microbes resistant to antibiotics and resistance genes in animal excreta, and antimicrobial residue found in animal-origin food and excreta are animals related contributive factors for the antibiotic resistance emergence and spread. Environmental factors including naturally existing resistance genes, improper disposal of unused antimicrobials, contamination from waste in public settings, animal farms, and pharmaceutical industries, and the use of agricultural and sanitation chemicals facilitatet its emergence and spread. Wildlife has a plausible role in the antimicrobial resistance spread. Adopting a one-health approach involving using antimicrobials properly in animals and humans, improving sanitation in public spaces and farms, and implementing coordinated governmental regulations is crucial for combating antimicrobial resistance. Collaborative and cooperative involvement of stakeholders in public, veterinary and ecological health sectors is foremost to circumvent the problem effectively.

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Anticoccidial and Antioxidant Activities of Moringa oleifera Leaf Extract on Murine Intestinal Eimeriosis

Cited by 10 publications

References 35 publications

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

An in vitro evaluation of the inhibitory effects of an aqueous extract of Acacia nilotica on Eimeria tenella.

Potential Causes of Spread of Antimicrobial Resistance and Preventive Measures in One Health Perspective-A Review

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