Anticorpos anti-C1q, anticromatina/nucleossomo e anti-dsDNA em pacientes com lúpus eritematoso sistêmico juvenil

Abstract

Objectives: To evaluate the presence of anti-C1q, anti-chromatin/nucleosome and anti-double stranded DNA (dsDNA) antibodies in juvenile systemic lupus erythematosus (JSLE) and controls. Methods: Sixty-seven JSLE and 34 healthy controls were analyzed for the presence of anti-C1q, anti-chromatin/nucleosome, and anti-dsDNA antibodies by ELISA. C1q levels were evaluated by radial immunodiffusion. Results: The mean current age was similar in JSLE patients and controls (14.6 ± 3.86 vs. 13.6 ± 2.93 years, P = 0.14). Higher frequencies of anti-C1q, anti-chromatin/nucleossome, and anti-dsDNA antibodies were observed in JSLE compared to controls (20% vs. 0%, P = 0.0037; 48% vs. 0%, P < 0.0001 and 69% vs. 3%, P < 0.0001, respectively). The median of anti-C1q, anti-chromatin/nucleossome, and anti-dsDNA antibodies were also signifi cantly higher in JSLE patients than in controls [9.6 (5.5-127) vs. 7.5 (5-20) units, P = 0.0006; 18 (1.9-212) vs. 3.2 (1.7-17) units, P < 0.0001; and 111 IU/mL (6-741) vs. 14 (6-33) IU/mL; P < 0.0001, respectively]. The sensitivity for anti-C1q, anti-chromatin/nucleosome, and anti-dsDNA antibodies was 21% (CI: 11-33), 49% (CI: 36-62), and 70% (CI: 57-81). The specifi city was 100% (CI: 88-100), 100% (88-100), and 97% (CI: 83-99), respectively. A positive correlation was found between anti-dsDNA levels and both anti-C1q (r = 0.51; CI: 0.29-0.68; P < 0.0001) and anti-chromatin/nucleosome antibodies (r = 0.87; CI: 0.79-0.92; P < 0.0001) levels. A negative correlation was observed between anti-C1q and C1q levels (r = −0.33; CI: −0.56-0.05; P = 0.018). The frequency of anti-dsDNA was higher in patients with SLEDAI-2K ≥1 (P = 0.0047) and no differences were observed in the frequencies of these three autoantibodies and nephritis (P > 0.05). Conclusion: Our study demonstrated an elevated specifi city for lupus diagnosis involving the three autoantibodies, especially anti-C1q and anti-chromatin/nucleosome.

Effect of chronic exposure to fine particulate matter on cardiac tissue of NZBWF1 mice

“…73,74 So another important characteristic marker of SLE is the production of anti-DNA antibodies that is directly associated with disease activity, being used by many studies as a parameter for diagnosis. 75,76 In this way, we performed an assay to detect these antibodies in our animals to verify the plausible interference of the exposure to PM2.5 in autoantibody production. We were expecting to obtain increased levels of anti-DNA antibodies.…”

Biomarkers Associated with Organ-Specific Involvement in Juvenile Systemic Lupus Erythematosus

“…Research into the role of autoantibodies as JSLE biomarkers showed that although autoantibodies such as anti-complement fraction 1q (anti C1q), anti-dsDNA and anti-chromatin/nucleosome antibodies were significantly higher in patients with JSLE compared to healthy controls, and in JSLE with any level of disease activity compared to remission, they did not associate with renal involvement [ 32 ].…”

Current International Guidelines for the Diagnosis and Treatment of Juvenile-Onset Systemic Lupus Erythematosus

“…С учетом более широкого спектра антител, выявляемых в детской популяции, на этапе диагностики СКВ с ювенильным дебютом настоятельно рекомендуется их исследование. Однако следует отметить, Международные и российские рекомендации по лечению ревматических заболеваний что на сегодняшний день не выявлено антител с определенными прогностическими качествами (например, предикторов тяжести течения заболевания, возможности формирования стойкой ремиссии, вовлечения определенных органов, риска повреждения), несмотря на многочисленные усилия по их поиску [33][34][35][36][37][38][39][40][41].…”