Antidermatophyte activity of ether extract of Nigella sativa and its active principle, thymoquinone

Aljabre, Salih H.M.; Randhawa, Mohamad Akram; Akhtar, Naeem; Alakloby, Omar M.; Al-Qurashi, Abdulrahman M; Aldossary, Ali

doi:10.1016/j.jep.2005.04.002

Cited by 113 publications

(64 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The MICs of the ether extract of N. sativa and TQ were between 10-40 and 0.125-0.250 mg/mL, respectively, while those of griseofulvin ranged from 0.00095 to 0.01550 mg/mL. These results denote the potentiality of N. sativa as a source for antidermatophyte drugs and support its use in folk medicine for the treatment of fungal skin infections [31] . The antiyeast activity of the black cumin seed quinines, dithymoquinone, thymohydroquinone, and TQ were evaluated in vitro with a broth microdilution method against six dairy spoilage yeast species.…”

Section: Antifungal Activitymentioning

confidence: 56%

A review on therapeutic potential of Nigella sativa: A miracle herb

Ahmad

Husain

Mujeeb

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

1,121

796

View full text Add to dashboard Cite

Section: Antifungal Activitymentioning

confidence: 56%

A review on therapeutic potential of Nigella sativa: A miracle herb

Ahmad

Husain

Mujeeb

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

1,121

796

View full text Add to dashboard Cite

“…However, other studies have shown that such extracts of these seeds could have an inhibitory effect on the growth of the yeast C. albicans in the liver, spleen and kidneys of infected mice [19] . This controversial results can be explained by: (a) The different techniques used for extraction [14,15,17] , (b) The differences between in vivo and in vitro studies [15,19] , (c) The sensitivity and the accuracy of the anti-microbial test, (d) The concentration and the effectiveness of the constituents in the extracts, (e) The conditions of seed collections and the season [15] , (f) The storage and the preservation method of the extracts and (g) Most previous studies stressed on the volatile oil and its constituent, thymoquinone [3,4,18,[20][21][22] . Three organic solvents were used to extract fixed oil constituents from seeds of N. sativa, these included: diethyl ether, chloroform and petroleum ether.…”

Section: Discussionmentioning

confidence: 99%

“…After agar solidification, the well (7 mm in diameter) was cut from the agar to produce a total of three wells per each agar plate. For preliminary test, six concentrations of each extract (10,20,30,40, 50 and 60% v/v) were prepared in a total volume of 100 µL per well using 99.5% analytical Dimethyl Sulphoxide (DMSO) as an organic solvent. Chloramphenicol (10 µg/well) and amphotericin B (10 µg/well) were used as positive standard antibiotics.…”

Section: Agar Well Diffusion Assaymentioning

confidence: 99%

“…Treatment of mice with the extract 24 h after intravenous injection of C. albicans caused a considerable inhibitory effect on the growth of the fungus in the liver, spleen and kidneys [19] . Moreover, the anti-fungal effects of ether extract and thymoquinone of N. sativa seeds against many species of dermatophytes, revealed the potentiality of such seeds as a source for anti-fungal drugs and support their use in folk medicine for the treatment of fungal skin infections [20][21][22] . The skin represents the first line of defense against microbial colonization and infection simply indicates the presence of microbes, which may be pathogens, opportunist or commensals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

View full text Add to dashboard Cite

Problem statement:The developing microbial resistance to the existing anti-microbial agents has become a real challenge and a serious problem facing patients suffering from skin infections. Seeds of Nigella sativa have been used for a long time in folk medicine for the treatment of such infections. Production of new potent agents is urgently needed, especially for hospitals and health centers. Therefore, the anti-microbial effect of aqueous, diethyl ether, chloroform and petroleum ether extracts of the seeds against four standard microbial strains and seven clinical isolates from patients with skin wound infections were investigated. Approach: The in vitro anti-microbial effect of the extracts at a concentration of 20% on standard strains and clinical isolates was assessed and compared with standard drugs, chloramphenicol and amphotericin B using agar well diffusion assay. The in vivo anti-bacterial effect of petroleum ether extract was studied in male BALB/c mice infected subcutaneously with S. aureus (ATCC 25923) or a clinical isolate (0.1 mL from 10 9 colony forming units mL −1 suspension) and immediately treated at the infected site by subcutaneous injection of 0.1 mL of pure extract (fixed oil) or chloramphenicol or normal saline. Counts of viable bacteria present in the skin area corresponding to the infected site were determined, after 24 and 48 h of infection and treatment. Results: The aqueous extract did not show any inhibitory effect against all the tested microorganisms. The diethyl ether and chloroform extracts indicated significant inhibitory effect only against Gram-positive bacteria. However, petroleum ether extract was proved to be the most powerful one against these bacteria and also against other clinical isolates like one Gram-negative bacterium (Klebsiella pneumonia) and the yeast (Candida albicans). Moreover, the extract revealed a superior effect over the standard drug, chloramphenicol, on the clearance of subcutaneous staphylococcal infection in mice when injected at the site of infection. Counts of viable bacteria were decreased at highly significant level in mice infected with S. aureus (ATCC 25923) or a clinical isolate. Conclusion/Recommendations:The results of this study revealed clear potentiality of N. sativa fixed oil as a source for anti-microbial drugs and support its use in folk medicine for the treatment of microbial skin infections.

show abstract

“…The seeds contain fixed oil, essential oil, alkaloids, steroids, and phenolic compounds (Akram Khan, 1999). Among a large number of previously isolated compounds, thymohydroquinone (ElAlfy et al, 1975) and thymoquinone (Aljabre et al, 2005) were identified as the main anti-microbial principles of the plant.…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-microbial activity of extracts from the callus cultures of some Nigella species

Landa¹,

Maršík

Vaněk

et al. 2006

Biologia

View full text Add to dashboard Cite

Crude methanol extracts from callus cultures of Nigella arvensis, N. damascena, N. hispanica, N. integrifolia, and N. sativa were investigated for their anti-microbial activity. Growth inhibition was determined in Gram-positive and Gram-negative bacterial strains as well as in yeast by using a broth-microdilution method. The results showed that the extracts of all calli tested exhibited significant anti-microbial activity, especially against Bacillus cereus, Staphylococcus aureus and Staphylococcus epidermidis. Compared with other Nigella species, a callus culture of N. hispanica was the most effective against the microorganisms used in this study.

show abstract

Antidermatophyte activity of ether extract of Nigella sativa and its active principle, thymoquinone

Cited by 113 publications

References 22 publications

A review on therapeutic potential of Nigella sativa: A miracle herb

A review on therapeutic potential of Nigella sativa: A miracle herb

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

In vitro anti-microbial activity of extracts from the callus cultures of some Nigella species

Contact Info

Product

Resources

About