Antidermatophyte and antioxidant activities of Nigella sativa alone and in combination with enilconazole in treatment of dermatophytosis in cattle

Balikci, Engin

doi:10.17221/32/2015-vetmed

Cited by 6 publications

(2 citation statements)

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“…Calves were observed daily for 6 weeks. In the beginning, during and after the treatment, the clinical efficacy was assessed by scoring dermatophytosis lesions on a 0–3 scale using previously published criteria [ 41 – 43 ] (Additional Table 2 ). The scoring was performed by the same investigator who was blinded to the treatment groups.…”

Section: Methodsmentioning

confidence: 99%

Ringworm in calves: risk factors, improved molecular diagnosis, and therapeutic efficacy of an Aloe vera gel extract

et al. 2020

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Background Dermatophytosis in calves is a major public and veterinary health concern worldwide because of its zoonotic potential and associated economic losses in cattle farms. However, this condition has lacked adequate attention; thus, to develop effective control measures, we determined ringworm prevalence, risk factors, and the direct-sample nested PCR diagnostic indices compared with the conventional methods of dermatophytes identification. Moreover, the phenolic composition of an Aloe vera gel extract (AGE) and its in vitro and in vivo antidermatophytic activity were evaluated and compared with those of antifungal drugs. Results Of the 760 calves examined, 55.79% (424/760) showed ringworm lesions; 84.91% (360/424) were positive for fungal elements in direct-microscopy, and 79.72% (338/424) were positive in culture. Trichophyton verrucosum was the most frequently identified dermatophyte (90.24%). The risk of dermatophytosis was higher in 4–6-month-old vs. 1-month-old calves (60% vs. 41%), and in summer and winter compared with spring and autumn seasons (66 and 54% vs. 48%). Poor hygienic conditions, intensive breeding systems, animal raising for meat production, parasitic infestation, crossbreeding, and newly purchased animals were statistically significant risk factors for dermatophytosis. One-step PCR targeting the conserved regions of the 18S and 28S genes achieved unequivocal identification of T. verrucosum and T. mentagrophytes in hair samples. Nested-PCR exhibited an excellent performance in all tested diagnostic indices and increased the species-specific detection of dermatophytes by 20% compared with culture. Terbinafine and miconazole were the most active antifungal agents for dermatophytes. Gallic acid, caffeic acid, chlorogenic acid, cinnamic acid, aloe-Emodin, quercetin, and rutin were the major phenolic compounds of AGE, as assessed using high-performance liquid chromatography (HPLC). These compounds increased and synergized the antidermatophytic activity of AGE. The treated groups showed significantly lower clinical scores vs. the control group (P < 0.05). The calves were successfully treated with topical AGE (500 ppm), resulting in clinical and mycological cure within 14–28 days of the experiment; however, the recovery was achieved earlier in the topical miconazole 2% and AGE plus oral terbinafine groups. Conclusions The nested PCR assay provided a rapid diagnostic tool for dermatophytosis and complemented the conventional methods for initiating targeted treatments for ringworm in calves. The recognized antidermatophytic potential of AGE is an advantageous addition to the therapeutic outcomes of commercial drugs.

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Section: Methodsmentioning

confidence: 99%

Ringworm in calves: risk factors, improved molecular diagnosis, and therapeutic efficacy of an Aloe vera gel extract

et al. 2020

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“…По результатам исследований E.Balikci, значительный положительный антидерматофитный и антиокси-дантный эффект наблюдался при использовании масла нигеллы посевной в ветеринарии при лечении дерматофитоза (Trichophyton verrucosum) у крупного рогатого скота [65]. Экстракты нигеллы посевной улучшают продуктивность и уменьшают колонизацию энтеропатогенов у домашней птицы, мелких жвачных животных и свиней [66].…”

Section: рис2 структурные формулы изохинолиновых и пиразоловых алкалоидов Fig 2 Structural Formulas Of Isoquinoline and Pyrazole Alkaloidunclassified

Nigella is a valuable economically useful crop (literature review)

Прохоров

2021

Ovoŝi Rossii

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The most important task for the sustainable development of modern agriculture is to increase the biological diversity of cultivated crops. In recent years, due to global climate change, the possibilities of using more thermophilic crops in new agro-ecological zones have expanded. The problem of increasing the biological diversity of vegetable products largely depends on the introduction of non-traditional plant species, with a simultaneous assessment of the initial material for its use in economically useful purposes and the creation of varieties and hybrids adapted to local conditions. In this regard, Nigella L. is of great interest among rare plant species. It is one of the most famous spicy-aromatic crops in the Mediterranean and Central Asia, possessing a wide range of biologically active compounds and unique medicinal properties. The review article provides information on the origin, biological characteristics and breeding of the culture. A brief review of food, medicinal and other economically useful properties is given, as well as the main elements of the technology for growing nigella are considered. It is shown that the cultivation of this culture will provide an opportunity to expand the range of non-traditional plants and be a promising renewable source of valuable plant raw materials, which will find application in various fields: in agriculture, in the food, pharmaceutical, perfumery and cosmetic industries, as well as in ornamental gardening.

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