Antidermatophytic Activity of Mikania micrantha Kunth: An Invasive Weed

Abstract

Context:The incidence of dermatophytosis has risen dramatically in recent years. Limited availability of side-effect free drugs has led to a search for new antidermatophytic agents.Objective:The objective was to investigate antidermatophytic activity and in vitro anti-inflammatory activity (protease inhibition assay) of whole plant (aerial parts only) of Mikania micrantha.Materials and Methods:The dried and powdered aerial parts of M. micrantha were extracted separately with petroleum ether, ethyl acetate and methanol. Antidermatophytic activity was determined by agar tube dilution method against Epidermophyton floccosum var. nigricans, Microsporum canis, Microsporum gypseum and Trichophyton rubrum. The activities of various parts of the plant – flowers, leaves and stem were separately analyzed using their ethyl acetate extract. Fungicidal efficacy and trypsin inhibiting activity of the whole plant, flowers and leaves were also analyzed using the ethyl acetate extracts.Statistical Analysis Used:For trypsin inhibition assay results are expressed as mean ± standard division. For antidermatophytic assay, the significance of the difference between control and test was analyzed statistically using Fisher's exact test.Results:Ethyl acetate extract of M. micrantha exhibited excellent antidermatophytic activity, followed by petroleum ether and methanolic extracts. Ethyl acetate extracts of whole plant, flowers, leaves and stem completely inhibited the growth of dermatophytes at the tested concentration of 2 mg/mL. Furthermore, ethyl acetate extracts of whole plant, leaves and flowers were fungicidal, and the percentages of trypsin inhibition exhibited were 33.73 ± 0.306, 39.0 ± 0.505 and 35.53 ± 0.503, respectively.Conclusions:Since M. micrantha possesses antidermatophytic as well as anti-inflammatory activities, the plant is an excellent candidate for the development of new medicaments against dermatophytoses in traditional as well as modern medicine.

Bioefficacy of Mikania micrantha H.B.K. against Water-Borne Bacterial Pathogens

“…Wei et al (2004), also reported and described some lavonoids and dicaffeoylquinic acid butyl esters as bioactive for M. micrantha. Since methanol is capable of extracting a wide variety of secondary metabolites; that is why methanolic extract sued to show more inhibition against bacterial pathogens (Jyothilakshmi et al 2017). Further, based on above indings, our results con irmed and made a clear observation that a relationship exists between the high activity of M. micrantha and the presence of various terpenes and phenolic components in leaves extracts.…”

Mikania micranthaKunth: An Ethnopharmacological Treasure Trove of Therapeutic Potential

“…[72] The ethyl acetate extract of the whole plant at 2 mg/mL completely terminated the growth of four dermatophyte stains, Microsporum canis, Epidermophyton floccosum, Trichophyton rubrum, and Microsporum gypseum. [62]…”

“…Many diseases, including dermatophytic infection, are locally treated with plants because of their ability to produce safe secondary metabolites to guard against infections [72] . The ethyl acetate extract of the whole plant at 2 mg/mL completely terminated the growth of four dermatophyte stains, Microsporum canis , Epidermophyton floccosum , Trichophyton rubrum , and Microsporum gypseum [62] …”

Chemical characterization, antimicrobial activity, and cytotoxic activity of Mikania micrantha Kunth flower essential oil from North East India

“…These compounds have inhibitory activity against microorganisms, viruses, and tumour cells. Though it has been used in the traditional medicines around the world, not much research work has been conducted so far on its medicinal properties till date, especially isolation and characterization of the chemical compounds responsible for different medicinal impacts like antimicrobial and cytotoxic activity (Jyothilakshmi et al 2015). A very few results were obtained regarding the analysis of M. micrantha flower essential oil and its biological activity (Shao et al 2001;Perez-Amador et al 2010).…”