Antidiabetic Activity of Extract Combination of Orthosiphon aristatus and Oryza sativa L. var glutinosa

Abstract

Traditionally and scientifically, research has shown that Orthosiphon aristatus and Oryza sativa L. var. glutinosa have antidiabetic activity. The combination of two medicinal plants can increase their biological activity. This study aimed to determine the antidiabetic activity of O. aristatus and O. sativa L. var. glutinosa on single and combined extracts. Phytochemical screening of the single extract was done qualitatively. The α-glucosidase inhibitory method was used as an antidiabetic activity. The results showed that every extract contained alkaloids, steroids/triterpenoids, flavonoids, tannins, quinones, and coumarins. A single extract of O. sativa L. var glutinosa, O. aristatus, and their combinations (1:1, 1:2, and 2:1) had an α-glucosidase enzyme inhibitory activity with an IC50 value of 67.82, 80.93, 73.81, 88.72, and 61.51 µg/ml, respectively. The combination shows that the ratio of 1:1 was nearly additive, 1:2 was slight to moderate antagonism, and 2:1 was moderate to slight synergism. The combination of 96% ethanol extract of O. sativa L. var. glutinosa and O. aristatus in a ratio of 2:1 was the most effective in increasing its inhibitory activity.

Antidiabetic Activity, Phytochemical Analysis, and Acute Oral Toxicity Test of Combined Ethanolic Extract of Syzygium polyanthum and Muntingia calabura Leaves

“…Studies have evaluated the α -glucosidase enzyme inhibitory activity of combination of extracts of Oryza sativa L. var glutinosa and Orthosiphon aristatus . Combinations of these extracts in a 1 : 1 ratio exhibited IC 50 values of 67.82 µ g/mL [ 10 ]. Furthermore, the ethanol extracts of turmeric, ginger, and black tea water extract were tested for their α -glucosidase inhibition.…”