Antidiabetic activity of Helicteres angustifolia root

Abstract

ContextThe root of Helicteres angustifolia L. (Sterculiaceae) has been used as folk herbal drug to treat cancer, bacterial infections, inflammatory, and flu in China. However, there is no report on its antidiabetic activity. Objective This study evaluates the antidiabetic activity of ethanol extract from H. angustifolia root. Materials and methods The promoting effect of H. angustifolia root ethanol extract (25, 50, and 100 mg/mL) on glucose uptake was evaluated using HepG2 cell, differentiated C2C12 myotubes, and differentiated 3T3-L1 adipocytes. The antidiabetic activity of the extract was assessed in vivo using STZ-induced diabetic rats by orally administration of the extract (200 and 400 mg/kg b.w.) once per day for 28 d. Blood glucose, TG, TC, TP, HDL-C, UA, BUN, AST, ALT, insulin, and HOMA-IR were analyzed. Results The results showed that the extract increased glucose uptake in C2C12 myotubes and 3T3-L1 adipocytes with an IC 50 value of 79.95 and 135.96 mg/mL, respectively. And about 12%, 19%, and 10% (p 5 0.05) in HepG2 cells when compared with the control at the concentration of 25, 50, and 100 mg/mL, respectively. After 28 days' treatment with the extract, significant reduction was observed in blood glucose, HOMA-IR, TC, TG, UA, BUN, AST, and ALT levels, while the levels of TP and HDL cholesterol increased. Discussion and conclusion These results suggest that H. angustifolia root ethanol extract possess potent antidiabetic activity, which is the first report on antidiabetic activity of this plant.

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An update on natural compounds in the remedy of diabetes mellitus: A systematic review

“…A 200 and 400 mg/kg/day dose for a period of 28 days in STZ-induced diabetic rats resulted in significant reduction in blood glucose, plasma insulin level and HOMA-IR in treatment group as compared to control group. 40 In rats receiving 400 mg/kg/day extract, the blood glucose, plasma insulin level and HOMA-IR were 23.86 ± 0.25mmol.L −1 , 6.98 ± 0.22μU.mL −1 and 7.41 ± 0.29, respectively whereas, those values in control diabetic group were 31.47 ± 0.30mmol.L −1 , 7.24 ± 0.38μU.mL −1 and 10.13 ± 0.56, respectively. 40 …”

“… 40 In rats receiving 400 mg/kg/day extract, the blood glucose, plasma insulin level and HOMA-IR were 23.86 ± 0.25mmol.L −1 , 6.98 ± 0.22μU.mL −1 and 7.41 ± 0.29, respectively whereas, those values in control diabetic group were 31.47 ± 0.30mmol.L −1 , 7.24 ± 0.38μU.mL −1 and 10.13 ± 0.56, respectively. 40 …”