Antidiabetic and antihyperlipidemic activity of hydroalcoholic extract of Withania coagulans Dunal dried fruit in experimental rat models

Abstract

Objective:Evaluation of antidiabetic potential of the hydroalcoholic extract of Withania coagulans Dunal dried fruit (WCDF) alone and in combination with glipizide, in streptozotocin-induced diabetes, and evaluation of possible antihyperlipidemic activity of the same extract in high-cholesterol diet-induced hyperlipidemia, in albino rats.Materials and Methods:Experimental diabetes was induced in 30 albino rats with intraperitoneal injection of streptozotocin (55 mg/kg). The rats were divided into five groups receiving the following treatments orally for 4 weeks: Vehicle, glipizide (2.5 mg/kg), WCDF extract (1000 mg/kg), WCDF extract (1000 mg/kg) plus glipizide (1 mg/kg) and WCDF extract (1000 mg/kg) plus glipizide (2.5 mg/kg). Fasting and postprandial blood glucose levels were measured every week for 4 weeks. Endocrine pancreas histopathology was done at the end. In a separate set of experiment, five groups of six albino rats each, received orally for 4 weeks, vehicle, cholesterol (25 mg/kg/day), cholesterol (25 mg/kg/day) plus atorvastatin (7.2 mg/kg/day), cholesterol (25 mg/kg/day) plus WCDF extract (1000 mg/kg/day) and no treatment, respectively. Estimation of serum lipid profile and liver histopathology was done at the end of 4 weeks.Statistical Analysis:Between-group and within-group comparisons were respectively done by analysis of variance (ANOVA) and repeated measures ANOVA, followed by post hoc Tukey's test, with a significance level of P < 0.05.Results and Conclusions:The 4-week treatment with WCDF extract significantly reversed hyperglycemia in streptozotocin-induced diabetes that was comparable to glipizide. When combined with glipizide (2.5 mg/kg), WCDF extract produced a synergistic antihyperglycemic effect as well as improvement in pancreatic histopathology. Moreover, hydroalcoholic extract of WCDF was effective and comparable to atorvastatin in controlling the high-cholesterol diet-induced hyperlipidemia in rats.

Antidiabetic, antihyperlipidemic activities and herb–drug interaction of a polyherbal formulation in streptozotocin induced diabetic rats

“…The serum collected on 21st day of the treatment period was also analyzed for total cholesterol (TC), high density lipoprotein cholesterol (HDLc) and triglyceride (TG) levels by using diagnostic kits. The low density lipoprotein cholesterol (LDLc) and very low density lipoprotein-cholesterol (VLDLc) were also calculated by using Friedwald's formula [2] .…”

“…Diabetes mellitus and its associated acute and long-term complications are an important health hazard globally. Its prevalence has now reached pandemic proportions in India [2] . It is reported that the four countries namely China, India, Indonesia and Japan from Asia are center for the global epidemic of diabetes and the number of diabetic patients worldwide has doubled in the past two decades [3] .…”

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

“…Upadhyay and et al reported antihyperglycemic effect of WCE in diabetic patients (1). Another study revealed anti-hyperlipidemia effects of WCE in rats (6). It has been reported that Withaferin A has protective effects against Alzheimer's disease (7).…”

Anti-hyperglycemic and anti-hyperlipidaemic effect of Arjunarishta in high-fat fed animals

“…Due to this reason, the need for novel treatment strategies which offer more benefits to patients suffering from T2DM was created, focusing on treatments that produce better glycemic control, appetite regulation, blood pressure, lipid reduction and weight loss. Also, there has been a growing emphasis on therapy of hyperlipidemia associated with diabetes to develop newer antidiabetic agents with potential anti-hyperlipidemic effects [4] .…”