Antidiabetic and antioxidant potential of <i>Gardenia latifolia</i> in type-2 diabetic rats fed with high-fat diet plus low-dose streptozotocin

Alshabi, Ali Mohamed; Shaikh, Ibrahim Ahmed

doi:10.15537/smj.2022.43.8.20220258

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…In recent years, gardenia was found to have many pharmacological benefits, such as anti-inflammatory effects, tumor prevention, cardiovascular disease prevention, and water-soluble nutrients that can be easily absorbed in the body [ 15 , 16 , 17 ]. One study showed that the gardenia latifolia extract (GLE) had an anti-diabetic potential in rats with a high-fat diet (HFD) + streptozotocin (STZ) induced type 2 diabetes mellitus (T2DM) [ 18 ]. However, there was less information about the functional mechanism of the hypoglycemic effects of crocin I, geniposide, from gardenia on T2DM in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

Effective Therapeutic Verification of Crocin I, Geniposide, and Gardenia (Gardenia jasminoides Ellis) on Type 2 Diabetes Mellitus In Vivo and In Vitro

Zhou

Zhang

Chen

et al. 2023

Foods

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For many centuries, Gardenia (Gardenia jasminoides Ellis) was highly valued as a food homologous Chinese herbal medicine with various bioactive compounds, including crocin I and geniposide. However, the functional mechanism underlying the hypoglycemic effect of gardenia is absent in the literature. To evaluate the effect of gardenia and its different extracts on type 2 diabetes mellitus (T2DM) in in vivo and in vitro experiments, the dried gardenia powder was extracted using 60% ethanol and eluted at different ethanol concentrations to obtain the corresponding purified fragments. After that, the active chemical compositions of the different purified gardenia fragments were analyzed using HPLC. Then, the hypoglycemic effects of the different purified gardenia fragments were compared using in vitro and in vivo experiments. Finally, the different extracts were characterized using UPLC-ESI-QTOF-MS/MS and the mass spectrometric fragmentation pathway of the two main compounds, geniposide and crocin I, were identified. The experimental results indicated that the inhibitory effect of the 40% EGJ (crocin I) on the α-glucosidase was better than the 20% EGJ (geniposide) in vitro. However, the inhibitory effect of geniposide on T2DM was better than crocin I in the animal experiments. The different results in vivo and in vitro presumed potentially different mechanisms between crocin I and geniposide on T2DM. This research demonstrated that the mechanism of hypoglycemia in vivo from geniposide is not only one target of the α-glucosidase but provides the experimental background for crocin I and the geniposide deep processing and utilization.

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Section: Introductionmentioning

confidence: 99%

Effective Therapeutic Verification of Crocin I, Geniposide, and Gardenia (Gardenia jasminoides Ellis) on Type 2 Diabetes Mellitus In Vivo and In Vitro

Zhou

Zhang

Chen

et al. 2023

Foods

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“…This disordered energy can, seemingly quite randomly, damage our cells, and result in oxidative-stress-related diseases such as cardiovascular disease, arthritis, cancer, atherosclerosis, aging, and neurological and neurodegenerative disorders [ 3 , 4 ]. In a healthy body, the oxidizing and reducing forces are—to a large extent—balanced and generate a reasonably controlled energy flow in which the accumulation of oxidative damage is kept within limits, and we can enjoy a lifespan of, on average, 80 years [ 5 ]. Intriguingly, the disordered energy also has a ‘good’ side, as it is involved in cell signaling, e.g., it can lead to adaptation of cells, making them more resilient [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…enjoy a lifespan of, on average, 80 years [5]. Intriguingly, the disordered energy also has a 'good' side, as it is involved in cell signaling, e.g., it can lead to adaptation of cells, making them more resilient [6].…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Antioxidant Activity of 2R,3R-dihydroquercetin

Xu,

Li,

Wang

et al. 2023

IJMS

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It has been reported that in an oxidative environment, the flavonoid 2R,3R-dihydroquercetin (2R,3R-DHQ) oxidizes into a product that rearranges to form quercetin. As quercetin is a very potent antioxidant, much better than 2R,3R-DHQ, this would be an intriguing form of targeting the antioxidant quercetin. The aim of the present study is to further elaborate on this targeting. We can confirm the previous observation that 2R,3R-DHQ is oxidized by horseradish peroxidase (HRP), with H2O2 as the oxidant. However, HPLC analysis revealed that no quercetin was formed, but instead an unstable oxidation product. The inclusion of glutathione (GSH) during the oxidation process resulted in the formation of a 2R,3R-DHQ-GSH adduct, as was identified using HPLC with IT-TOF/MS detection. GSH adducts appeared on the B-ring of the 2R,3R-DHQ quinone, indicating that during oxidation, the B-ring is oxidized from a catechol to form a quinone group. Ascorbate could reduce the quinone back to 2R,3R-DHQ. No 2S,3R-DHQ was detected after the reduction by ascorbate, indicating that a possible epimerization of 2R,3R-DHQ quinone to 2S,3R-DHQ quinone does not occur. The fact that no epimerization of the oxidized product of 2R,3R-DHQ is observed, and that GSH adducts the oxidized product of 2R,3R-DHQ on the B-ring, led us to conclude that the redox-modulating activity of 2R,3R-DHQ quinone resides in its B-ring. This could be confirmed by chemical calculation. Apparently, the administration of 2R,3R-DHQ in an oxidative environment does not result in ‘biotargeting’ quercetin.

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“…It is interesting that complications may develop in some patients despite being under medical control. Therefore, alternative appropriate treatment methods should be developed to treat the disease and prevent complications [2]. Oxidative stress has been noted in the pathogenesis of diabetes, since some complications cannot be prevented despite treatment with insulin or antihyperglycemic drugs.…”

Section: Introductionmentioning

confidence: 99%

Importance of curcumin effect and asprosin level on glucose metabolism in diabetic rats

Gokdemir¹,

author²,

Gökdemir³

et al. 2023

Med-Science

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Asprosin is a new hormone secreted mainly from white adipose tissue. It may be associated with the pathogenesis of obesity, diabetes and some metabolic diseases. The changes in plasma asprosin levels of experimental diabetic rats and the relation of these changes with liver glucose metabolism and some diabetes parameters were investigated, and the effects of metformin, gliclazide or curcumin treatment on plasma asprosin levels were tried. The study was designed as an animal model in diabetic rats The albino rats were divided into five groups. To induce diabetes, a single dose of STZ was injected intraperitoneally. Diabetics rats were treated intragastrically with metformin (D+Metformin group), gliclazide (D+Giliclazide group) or 20 curcumin (D+Curcumin group) for eight weeks. Fasting blood glucose, insulin levels and other parameters were measured. Plasma asporsin levels of untreated diabetic rats increased significantly (P<.001). Although the plasma asprosin levels of diabetic rats treated with the rugs were significantly lower (P<.001). Fasting blood glucose levels of diabetic rats treated with the drugs were found to be remarkably lower than the diabetic control values (P<.001, respectively). There was no significant difference in the insulin levels and HOMA-IR between these three groups. Curcumin treatment provides significant improvements in plasma asprosin level and diabetes parameters. The increase in plasma asprosin level in diabetic rats may be one of the main reasons that facilitate the development of the disease or is responsible for its pathogenesis. Our findings support the idea that curcumin may be an important treatment option for diabetes.

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Antidiabetic and antioxidant potential of Gardenia latifolia in type-2 diabetic rats fed with high-fat diet plus low-dose streptozotocin

Cited by 5 publications

References 36 publications

Effective Therapeutic Verification of Crocin I, Geniposide, and Gardenia (Gardenia jasminoides Ellis) on Type 2 Diabetes Mellitus In Vivo and In Vitro

Effective Therapeutic Verification of Crocin I, Geniposide, and Gardenia (Gardenia jasminoides Ellis) on Type 2 Diabetes Mellitus In Vivo and In Vitro

Unraveling the Antioxidant Activity of 2R,3R-dihydroquercetin

Importance of curcumin effect and asprosin level on glucose metabolism in diabetic rats

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