Antidiabetic and hypolipidemic effects of adropinin streoptozotocin-induced type 2 diabetic rats

Abstract

BACKGROUND: To examine the effects of adropin on glucose and lipid metabolism in a rat model of Type 2 diabetes mellitus (T2DM). METHODS: T2DM were established using high-fat diet and streptozocin (STZ; 35 mg/kg/b.w.). Seven days after STZ induction, diabetic rats were randomly treated with adropin (2.1 μg/kg/day intraperitonealy) for 10 days. The study involved the evaluation of biochemical parameters, including blood glucose, total cholesterol (TC), triglycerides (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) activities. Additionally, Tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6) and inducible nitric oxide synthase (iNOS) mRNA gene expressions in pancreas tissue were determined by reverse transcription-polymerase chain reaction. RESULTS: The serum levels of insulin and adropin were determined by ELISA. Treatment with adropin showed a signifi cant reduction in blood glucose levels, HbA1c (%), HOMA-IR and increase in HOMA-β, serum insulin levels. In addition, intraperitoneal adropin application can reduce serum levels of TC, TG, LDL-C, and increase level of HDL-C. Adropin also effectively ameliorated the alterations in TNF-α, IL-6 and iNOS mRNA expression. CONCLUSION: The present study indicates that the adropin possesses antidiabetic and antidyslipidemic effects in T2DM (Tab. 2, Fig. 3, Ref. 32). Text in PDF www.elis.sk. KEY WORDS: adropin, type 2 diabetic rat, blood glucose, insulin, high fat diet.

Adropin and irisin in arterial hypertension, diabetes mellitus and chronic kidney disease

“…50 Adropin may play an important anti-inflammatory function in DM patients by reducing the mRNA expression of interleukin 6 and TNF-α. 50,51 Interestingly, a study published in 2016 focusing on adropin-associated genes in hemodialysis patients showed lower adropin concentrations in RXRA homozygotes (rs749759 and rs10776909) as opposed to an EHNO gene (rs2281997), where the adropin concentration was higher. 52…”

The Effect of Ghrelin Treatment on Cell Survival and Inflammation in Type 2 Diabetic Rat Liver

“…IL-6 concentration in blood increases in type 2 diabetes (31) . According to study on rats, IL-6 mRNA expression of pancreas is higher in STZ-induced diabetes as compared to healthy rats (32) . PPAR-γ, a transcription factor, is a member of the nuclear receptor superfamily.…”

Adropin in women with polycystic ovary syndrome

“…The animal studies suggested that adropin had an impact on fat metabolism. Akciar et al demonstrated that adropin administered parenterally reduced the total cholesterol, TG, and LDL cholesterol levels and increased HDL cholesterol levels in mice with DM [23]. Butler, in his study in obese patients, reported that serum adropin levels were negatively correlated with TG and LDL cholesterol levels and positively correlated with HDL cholesterol and FFA levels [10].…”

“…They demonstrated that the adropin administration caused a significant decrease of fasting glucose level, HOMA-IR, and HbA 1c . The authors suggested that adropin could improve glucose metabolism and insulin sensitivity by inhibition the production of inflammatory cytokines [23].…”