Antidiabetic effect of a leaf extract from Gymnema sylvestre in non-insulin-dependent diabetes mellitus patients

Baskaran, K.; Ahamath, B.Kizar; Shanmugasundaram, K. Radha; Shanmugasundaram, E. R. B.

doi:10.1016/0378-8741(90)90108-6

Cited by 264 publications

(166 citation statements)

References 14 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…There are reports that G. sylvestre leaf extracts reduce hyperglycaemia in diabetic rabbits (Shanmugasundaram et al 1983), rats (Srivastava et al 1985) and humans (Khare et al 1983, Baskaran et al 1990, and these glucose-decreasing effects may be mediated by increases in insulin secretion (Shanmugasundaram & Panneerselvam 1981). There has been little systematic characterisation of the identities of the insulinotropic agents within the leaf extracts, but aqueous ethanolic extractions of the leaves provide two potentially active fractions, one containing conduritol A, an acid-soluble polyol-polyhydroxy cyclic compound (Miyatake et al 1993), and the other containing a mixture of acidinsoluble triterpenoid saponins (gymnemic acids), designated GS3 and GS4 ).…”

Section: Introductionmentioning

confidence: 99%

“…Most studies have been performed using GS4, which is purified from GS3 by reprecipitation with acid of GS3 solubilised in alkali ). GS4 has been used clinically to treat NIDDM, and it was shown to increase serum insulin concentrations, normalise blood glucose concentrations and reduce the requirement for sulphonylurea (Baskaran et al 1990). The potential use of GS4 in the treatment of diabetes is intriguing, and in the current study we have examined whether GS4, prepared according to a previously described method , exerts insulinotropic effects on -cell lines and on isolated islets of Langerhans.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability

Persaud

Al-Majed²,

Raman³

et al. 1999

Journal of Endocrinology

166

View full text Add to dashboard Cite

To determine whether extracts of Gymnema sylvestre may have therapeutic potential for the treatment of noninsulin-dependent diabetes mellitus (NIDDM), we examined the effects of an alcoholic extract of G. sylvestre (GS4) on insulin secretion from rat islets of Langerhans and several pancreatic -cell lines. GS4 stimulated insulin release from HIT-T15, MIN6 and RINm5F -cells and from islets in the absence of any other stimulus, and GS4-stimulated insulin secretion was inhibited in the presence of 1 mM EGTA. Blockade of voltage-operated Ca 2+ channels with 10 µM isradipine did not significantly affect GS4-induced secretion, and insulin release in response to GS4 was independent of incubation temperature. Examination of islet and -cell integrity after exposure to GS4, by trypan blue exclusion, indicated that concentrations of GS4 that stimulated insulin secretion also caused increased uptake of dye. Two gymnemic acidenriched fractions of GS4, obtained by size exclusion and silica gel chromatography, also caused increases in insulin secretion concomitant with increased trypan blue uptake. These results confirm the stimulatory effects of G. sylvestre on insulin release, but indicate that GS4 acts by increasing cell permeability, rather than by stimulating exocytosis by regulated pathways. Thus the suitability of GS4 as a potential novel treatment for NIDDM can not be assessed by direct measurements of -cell function in vitro.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability

Persaud

Al-Majed²,

Raman³

et al. 1999

Journal of Endocrinology

166

View full text Add to dashboard Cite

show abstract

“…[4][5][6] Various effects have been reported, such as suppression of glucose absorption in the small intestine of rats, reduction of plasma glucose increment in the oral sucrose tolerance test, significantly lowered blood glucose and insulin values in dogs as well as suppression of insulin increase in glucose tolerance tests in men and the alleviation of diabetic symptoms in patients with non-insulin-dependent diabetes mellitus. 7,8) As for the active substances involved in G. sylvestre, the triterpenoid saponin and its derivatives have been identified. These are glycosides where gymnemagenin is formed by attachment of glucronic acid to the triterpenoid structure as aglycone.…”

mentioning

confidence: 99%

Effect of Long Term-Administration with Gymnema sylvestre R. BR on Plasma and Liver Lipid in Rats.

Shigematsu

Asano

Shimosaka

et al. 2001

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Extract of Gymnema sylvestre leaves was administered to rats receiving either a high fat diet or normal fat diet for 10 weeks to investigate its influence on plasma and liver lipids and on visceral fat accumulation. In addition, its effect was compared with those of chitosan and the influence of combined use of these two substances was also evaluated. Within the high fat diet groups, the extract suppressed body weight gain and accumulation of liver lipids to the same extent as chitosan and the combined use. In addition, intraperitoneal fat and fat drop vacuoles on the epithelium of renal tubules, noted in the high fat diet group, were scattered by administration of the extract with the same results as for chitosan and combined use. Within the normal fat diet groups, plasma triglyceride levels decreased by administration of the extract, with similar results as chitosan and combined use. Concerning plasma total cholesterol, there was no decreasing effects with the extract, as found with chitosan and combined use. However, the effect of chitosan on plasma total cholesterol tended to be enhanced when used in combination with the extract. In addition, long-term administration of the extract did not show any influence on hematological and blood chemical parameters.

show abstract

“…É uma planta tropical que cresce nas florestas da Índia e, nesse país, tem sido usada há mais de 2000 anos no tratamento do Diabetes melltus (DM). Este uso popular foi parcialmente comprovado em estudos realizados em humanos e animais de laboratório (Baskaran et al, 1990;Shanmugasundaran et al, 1990;Chattopadhyay, 1999).…”

Section: Introductionunclassified

Efeito da Gymnema sylvestre na elevação da lipidemia após administração de gordura de origem animal em ratos wistar

Silva¹,

Bazotte²,

Vasconcelos³

et al. 2004

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

RESUMO.Neste trabalho investigamos a influência da Gymnema sylvestre (Gs) na elevação da lipidemia após administração de gordura de origem animal em ratos machos Wistar (230g-250g). Os animais, em jejum por 22 horas, foram divididos em 5 grupos que receberam via intragástrica às 8h os seguintes tratamentos: 1) 2mL de gordura suína Batavo ® /kg de peso corpóreo (GSB); 2) GSB + 500mg/kg de peso corpóreo de Gs (Gs 500mg); 3) GSB + 1g/kg de peso corpóreo de Gs (Gs 1g); 4) Gs 500mg; 5) Gs 1g. Os animais foram sacrificados 30min após a administração destas substâncias. Não observamos diferenças significativas na concentração sérica de lipídeos totais, triglicerídeos e colesterol total nos ratos que receberam GSB na ausência ou presença de Gs 500mg ou Gs 1g. Portanto, nossos resultados sugerem que a Gs não afeta a absorção intestinal de lipídeos.Palavras-chave: Gymnema sylvestre, absorção de lipídeos, ratos Wistar.ABSTRACT. The influence of Gymnema sylvestre on the elevation of blood levels of lipids after administration of animal fat in male wistar rats. The aim of the present experiment was to investigate the influence of Gymnema sylvestre (Gs) on the elevation of blood levels of lipids after administration of animal fat in male Wistar rats (230-250g). The animals, which were fasted for 22h, were separated in five groups that received (8:00 a.m) intragastric administration of: 1) 2ml of pork fat Batavo ® /kg of body weight (PFB); 2) PFB+500mg/kg of Gs body weight (Gs 500mg); 3) PFB + 1g/kg of Gs body weight (Gs 1 g); 4) Gs 500mg; 5) Gs 1g. The animals were sacrificed 30min after the administration of these substances. No significant differences were verified in the serum concentration of total lipids, triglycerides and cholesterol in the animals, which received PBF in the presence or absence of Gs 500mg or Gs 1g. Therefore, the results showed that Gs could not affect the intestinal absorption of lipids.

show abstract

Antidiabetic effect of a leaf extract from Gymnema sylvestre in non-insulin-dependent diabetes mellitus patients

Cited by 264 publications

References 14 publications

Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability

Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability

Effect of Long Term-Administration with Gymnema sylvestre R. BR on Plasma and Liver Lipid in Rats.

Efeito da Gymnema sylvestre na elevação da lipidemia após administração de gordura de origem animal em ratos wistar

Contact Info

Product

Resources

About